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Chapter Ten Hygiene in the Laboratory Animal Facility.

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Presentation on theme: "Chapter Ten Hygiene in the Laboratory Animal Facility."— Presentation transcript:

1 Chapter Ten Hygiene in the Laboratory Animal Facility

2  If viewing this in PowerPoint, use the icon to run the show (bottom left of screen).  Mac users go to “Slide Show > View Show” in menu bar  Click on the Audio icon: when it appears on the left of the slide to hear the narration.  From “File > Print” in the menu bar, choose “notes pages”, “slides 3 per page” or “outline view” for taking notes as you listen and watch the presentation.  Start your own notebook with a 3 ring binder, for later study! ALAT Presentations Study Tips

3 Sterilization, Disinfection & Sanitization  Difference between sterilization, disinfection & sanitization is a matter of degree of cleanliness.  Sterilization = destruction of all organisms on an object  Disinfection = reduction of number of pathogenic microorganisms on an object to a harmless level  Sanitization = reduction of number of microorganisms on an object to acceptable public health standard  Sanitization = make an object aesthetically pleasing & clean

4 Sterilization  Autoclaves sterilize by exposure to moisture at high temperature & pressure.  Autoclaving is fast, reliable, relatively cheap.  Autoclaving avoids the use of toxic chemicals.

5 Autoclaving  Microorganisms, type & size of material being sterilized determines autoclaving time.  large bags of bedding, nested shoebox cages or heavily wrapped surgical instruments  Items should be autoclaved for at least 15 min. at 250°F & with a pressure of 15 psi.  High pressure allows steam to be superheated.  Some items cannot be autoclaved because of sensitivity to heat, moisture or high pressure.  Other sterilization techniques include:  ethylene oxide gas, dry heat sterilization, chemical sterilization, gamma irradiation & liquid filtration

6 Disinfection  Disinfectants too strong for use on living animals.  Classified according to type of microorganism they are most effective against.  End in “–cidal” has killing action.  End in “–static” inhibits microorganism growth.  Bacteriostat prevents growth, does not necessarily kill.  Bactericidal kills bacteria, not necessarily spores.  Sporicidal kills spores & bacteria.  Chemicals, such as phenols, bleach & quaternary ammonia disinfect objects.

7 Disinfection II  Bleach is a superior disinfectant.  It kills many types of bacteria & viruses, is inexpensive & available.  It does not contain dirt-loosening detergents.  < effective if surface not cleaned of organic matter.  Phenolic compounds were popular disinfectants.  High concentrations needed to produce disinfection.  Cats react adversely to phenol.  Quaternary ammonia compounds are weak.  Destroy cell membranes of certain types of microorganisms  Available as virucides, algicides or fungicides  Less effective when mixed w/ detergents or soaps.  Combination neutralizes disinfecting capability.

8 Sanitization  Number of bacteria on inanimate objects reduced enough to prevent disease.  Routine cleaning of items such as floors, cages, walls, feeders, sinks, implements & tables  Removes dirt, hair, dust, saliva, blood, feces, urine.  Wash w/ detergent & rinse w/ water at 180°F.  Deodorants not used in place of sanitization.  Indiscriminate mixing of chemicals can cause reactions hazardous to animals & people.

9 Chemical Guidelines  Guidelines concerning handling of chemicals:  Store chemicals in a cool, central area.  Follow the instructions on label.  Never use a container that is not labeled.  Do not make an assumption as to contents.  Never mix two chemicals together unless authorized by manufacturer.  Mixing ammonia & bleach produces a toxic gas.

10 Cage Changes  The Guide:  Cages should be changed often enough “to provide a healthy environment for animal, in accord with its normal behavior and physiologic characteristics.”  Generally change solid-bottom cages 1-2 x/wk.  Generally drop-bottom cages changed 1x/2 wks.  Number of animals in a cage, cage size & type of bedding affect change cage frequency.  Larger animals (dogs, cats, nonhuman primates) - daily cleaning & bedding replacement.  AWA reg’s set minimum cleaning standards for cages, feeders & water bottles.

11 Equipment Cleaning Techniques  No hand cleaning in animal room.  Airborne microorganisms contaminate environment.  Pass-through washers have clean & dirty side.  Soiled equipment delivered to dirty side.  Cages scraped free of most bedding, feces & debris.  Chemical descalers remove most of urine scale.  Feeders & dishes are scrubbed free of debris.  Bottle brush loosens deposits inside bottles.  Sipper tubes rinsed, washed in a cage washer.  Equipment too large to fit into cage washers should be vigorously hand-scrubbed or pressure washed, using detergents and disinfectants.

12 (Image) Washing Dog Run Flooring

13 Room Cleaning Schedules & Techniques  Sinks - clean, free of clutter & stocked.  Vents and doors - kept free of dust, grime, hair... clogged vents reduce air circulation.  Trash cans - disposable plastic liners, emptied frequently, & disinfected regularly.  Animal rooms - emptied, cleaned & disinfected on fixed schedule.  Clean walls, ceilings, lights & all exposed surfaces.  Clean sink, broom, mop & bucket after each use.  Replace or launder mop heads frequently.  Keep cleaning items in room where they are used.  Use ONLY for cleaning of that room.  Reduces chance of spreading disease from room to room.

14 (Images) Room Cleaning

15 Other Equipment Changes  Racks w/ built-in cages - wash > 2 x/mo.  Cleaning feeders depend on number & type of animals being fed & type of diet.  Critical to inspect water bottles, automatic valves & sipper tubes daily to be sure working.  Replace used water bottles w/ sanitized bottles.  Put refilled bottles back on cages from which they came to prevent cross-contamination.  Watering equipment should be thoroughly flushed before reconnecting rack to room water lines.  Racks should be stored w/ empty water lines.

16 Environmental Monitoring  Temp tapes used to evaluate efficiency of sanitization.  Temp indicator monitors for proper washing & rinsing temps.  One type is a strip labeled w/ heat-sensitive indicator.  Indicator is attached to surface of equipment, which is then sent through washer.  Strip indicates highest water temp on surface.  Rinse water temperature should reach at least 180°F.

17 Environmental Monitoring II  Bacterial culture - testing for bacteria on surface of clean cages & equipment that have been through cage washer is also a common monitoring method.  Small plastic dishes containing a nutrient substance suitable for bacterial growth are pressed onto surface of a clean area.  Small plastic dish is placed in an incubator or allowed to sit at room temp 24 hrs.  Bacteria present on cage surface will grow on nutrient substance & indicate effectiveness of cleaning procedure.

18 Vermin Control  A properly constructed building, good housekeeping program & proper waste disposal help control vermin populations.  flies, fleas, cockroaches, ticks, wild rodents...  Vermin enter on feed, bedding, and humans, and through cracks & small openings.  Keep areas sanitary, dispose of food & bedding, close doors & seal cracks.  Wild rodents that enter a facility must be trapped.  Use of pesticides in animal areas should be strictly controlled.

19 Pesticides  Investigators must consent to their use, as pesticides, just like deodorants, can compromise experimental results.  These chemicals must not be allowed to contact the animals or their feed, bedding or water.  As part of a comprehensive control program, relatively harmless chemicals, such as boric acid and amorphous silica, can help control cockroach infestations.

20 Personal Safety & Hygiene  Protective clothing:  prevents contact w/ infectious, toxic or corrosive agents  type needed depends on procedures being performed  Non-slip bottoms & steel-toed shoes offer protection against slipping as well as from dropped equipment injuries.  Disposable shoe covers prevent cross- contamination in germ free, quarantine & isolation areas.  Only wear work shoes in facility.  Ear protectors recommended in noisy areas in which average noise level is >85 decibels.

21 Goggles & Uniforms  Goggles offer the best protection, because they cover the entire eye and surrounding area.  Facilities should also have eyewash stations in areas where chemical splashes could occur.  Closely fitted face masks are most effective in preventing personnel from inhaling contaminants.  Street clothes should not be worn while working in an animal facility, and uniforms should not be worn outside the facility.  Uniforms protect personnel & environment outside facility against contamination.

22 Gloves  Leather gloves of various lengths are worn to handle animals that bite or scratch.  Some reinforced w/ metal for bite protection.  Heat-resistant to handle hot items or dry ice.  People w/ contact skin allergies to animals should wear disposable plastic or latex gloves.  Special gloves are available for people who have allergies to latex or powder.

23 Personal Hygiene Practices  Safety and personal hygiene guidelines:  Store & consume food in designated areas of facility.  Keep hands away from mouth, nose, eyes, face & hair.  Smoke only in assigned areas.  Always wash hands:  after removing dirty PPE  before applying makeup, smoking or eating  before leaving animal room or cage washing area  Do not wear jewelry that interferes w/ hand washing.  Some facilities require employees to shower before entering and/or exiting animal areas.

24 Occupational Health Program  A pre-employment physical exam, medical history & vaccinations are part of program,  Tetanus - spore-forming bacteria in environment.  Personnel who handle animals or clean up after them are exposed to these spores.  Infection follows deep puncture wounds which are difficult to clean by conventional washing.  Rabies - virus which can occur in any mammal  Personnel who handle animals obtained from animal shelters should be inoculated w/ pre-exposure vaccine.  Hepatitis - virus that infects liver.  Most cases acquired from from nonhuman primates.  Personnel who work w/ primates are often vaccinated

25 Occupational Health Program II  Zoonotic agents are infectious agents that can be transmitted from animals humans.  TB, measles & salmonellosis  Personnel who work with NHP run a higher risk of exposure to TB.  Handlers have tuberculosis tests performed regularly.  All injuries & accidents must be reported to supervisor, regardless of how insignificant.  Immediately wash bite wound w/ soap & water.  Notify supervisor of bite as quickly as possible.  Report location of bite and animal that bit them.

26 Occupational Health Training  In addition to providing protective equipment and vaccinations, a research facility is also required to provide training of personnel in the areas of proper use of equipment, zoonotic diseases, blood-borne pathogens and other areas which can be hazardous to employees.

27 Additional Reading Biosafety in Microbiological and Biomedical Laboratories. HHS Publication No. (CDC) rd. Edition US Government Printing Office, Washington, D.C Occupational Health and Safety in the Care and Use of Research Animals. National Research Council. National Academy Press, Washington, D.C


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