4 Laboratory Laboratory coat is required Hair should be tied back No open shoes( sandals)No Gum, food, beveragesKeep personal belongings on shelves at the entrance to the laboratoryPrepare for each class by reading laboratory experiment(s) for class
5 Journal For the second class you will need a Journal The Journal can be a marble notebook or spiral bound notebook. It will consist of laboratory experiments for each class.Directions for your laboratory manual will be provided during the second class
7 Introducing your microscope Always clean lenses before startingAlways with both hands supporting baseStore with arm facing the door. CoverCleaning lenses before and after usingFocus with scanning and low before proceeding to high and oilLearn the most effective ways to regulate and manage light
8 Objective Lenses Objective Lens Mag Total Mag Color of Lens Use Scaniing550RedObserve the entire slide. Focus with coarse adjustmentLow10100YellowTweak focus with coarse. Make sure the image is clear and centeredHigh40400BlueFocus only with fine adjustmentOil1000WhiteApply oil and then turn high power objective lens and focus with fine adj
9 Microscope terms Microscope terms Magnification Total magnification ResolutionDepth of fieldSize of fieldWorking distanceParfocalParcentric
10 Microscope and Slides Three Threads Place slide on mechanical stage. Make sure that stage clips are holding slide securely. Use the scanning lens( red) to find the point at which the threads cross.Focus the microscope . Using the coarse adjustmentObserve and drawChange to low power. Turn the revolving nose piece so that the yellow lens is in place. Observe and draw
11 Observations What do you notice about the size of field????? What do you notice about your ability to focus.Focus up and down very carefully and slowly with the fine adjustmentWhat occurs? Why
12 High PowerIf your focus is sharp and clear, turn the revolving nosepiece to blueUse the fine adjustment, focus carefully – you are very close to the slide.Observe and draw
13 Bacterial Slide Take a slide of a bacillus from the supply area Place it on the StageRepeat the procedure, for focusingAdjust the light and drawObserve and draw.
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