1. 10X dilution x ~10nM full – length competitor
Cell extracts
DGal4 WT
Myc-Gal4-ER-VP16
Myc-Gal4-ER
[GST-Gal4-VP16] nM
100 20 2
0.2
0.02
anti – Gal4 N-term
10X dilution x ~10nM full – length competitor
~100 nM [Competitor]
Nalley, et al. Figure S1.

2. a. b. anti-Gal4 anti-Myc Gly/Lac Galactose PUT2 promoter PUT2 promoter-1 1 5 15 30 60
Time after E2: b. -1 1 5 15 30 60
Time after E2:
PUT2 promoter
Nalley, et al. Figure S2

3. a. b.
Nalley, et al. Figure S3

4. a. b. anti-Rb anti-Gal4 Total Min. after E2 Nalley, et al. Figure S430 60
120
180
240
anti-Rb
anti-Gal4
Total b.
Nalley, et al. Figure S4

5. a. b. c. IP Total anti-Gal4 anti-Myc Time after E2 -60 -1 Dgal4 -60 -15 15 30 IP
Total
gly/lac
gly/lac
gly/lac
galactose
galactose b. c.
Nalley, et al. Figure S5

6. A. DMSO MG132 ise1 Dpdr5 B. Min. after Gal Addition
-30 30 60 90
120
180
-30 30 60 90
120
180
ise1
-30 30 60 90
120
180
-30 30 60 90
120
180
Dpdr5 B.
Nalley, et al. Figure S6