1. a c b S1. Lee et al., 2011 * RRT-binding PXDLS-binding NAD(H)-binding
S422A
PXDLS-binding
NAD(H)-binding
PXDLS-binding
K428R
G183V
Myc/His
CtBP1 1 27
121
129
181
204
274
327
352
440
Myc/His
Myc/His-CtBP1 FL
Myc/His
Myc/His-CtBP1/1-140
Myc/His
Myc/His-CtBP1/1-369
Myc/His
Myc/His-CtBP1/
Myc/His
Myc/His-CtBP1/
Myc/His
Myc/His-CtBP1/
Myc/His-pcDNA
6Myc-XIAP/
6Myc-XIAP/
6Myc-XIAP/
6Myc-XIAP/FL c
6Myc-XIAP/FL b
6Myc-XIAP/
IP : α-CtBP1
IB : α-Myc *
6Myc-XIAP/
6Myc
BIR1
BIR2
BIR3
RING
6Myc-XIAP/FL 1 28 95
164
232
266
331
450
484
497
6Myc
BIR2
BIR3
RING
6Myc-XIAP/
6Myc-XIAP/FL
6Myc
BIR3
RING
6Myc-XIAP/
6Myc-XIAP/
6Myc
RING
6Myc-XIAP/
Lysates
6Myc-XIAP/
6Myc-XIAP/
CtBP1
S1. Lee et al., 2011

2. a b S2. Lee et al., 2011 DMSO MG132 MG132 HA-Ub Myc/His-pcDNA
6Myc-XIAP
6Myc-cIAP1 - - + - + - + + - + -
dCtBP-V5
GST-Ub
GFP-Diap1 WT
GFP-Diap1 th6 + - + + + - + - + + + -
dCtBP-(Ub)n
IP : α-V5
IB : α-V5
IP : α-CtBP1
IB : α-HA
CtBP1-(Ub)n *
dCtBP-V5
6Myc-cIAP1
6Myc-XIAP
Lysates
Lysates
GFP-Diap1 WT/th6
dCtBP-V5
CtBP1
S2. Lee et al., 2011

3. Supplemental Figure Legends
Supplemental Figure 1: (a) Schematic diagram of various CtBP1 domain constructs (b) Schematic diagram of various XIAP deletion constructs (c) Interaction of CtBP1 with various XIAP deletion mutants. HeLa cells were transfected with control or 6Myc-XIAP deletion constructs shown in (S1b). BIR3 is responsible for the interaction with CtBP1. Supplemental Figure 2: (a) Comparison of dCtBP ubiquitination by Diap1 WT or th6 RING mutant (b) In vivo ubiquitination in HeLa cell. 6Myc-XIAP or 6Myc-cIAP1 was co-transfected with HA-Ub. After 24h transfection, cells were treated with DMSO or 20 uM MG132 for 6h.