1. Generation and characterization of Vps15‐null mice and cells. A
Generation and characterization of Vps15‐null mice and cells.A.Schematic representation of the targeted allele of Vps15 gene and Vps15 protein domain structure.
Generation and characterization of Vps15‐null mice and cells.A.Schematic representation of the targeted allele of Vps15 gene and Vps15 protein domain structure. The coding exons are depicted by black boxes. The grey triangles denote loxP sequences, black triangle—FRT sequence. The putative protein kinase domain, HEAT domains (Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A) and the yeast kinase TOR1) and repeats of WD domain in the full‐length and truncated Vps15 are depicted.B.Immunoblot analysis of Vps15, Vps34 and Beclin 1 expression in MEF cells transduced with Adeno‐Cre virus analysed 72 h post‐infection.C.Vps15‐depleted MEFs were transfected with 2xFYVE‐GFP expressing plasmid. 36 h post‐transfection cells were treated with 200 nM wortmannin or DMSO alone for 30 min and PI3P positive compartments were visualised by confocal microscopy. Scale bars: 20 µm.
Ivan Nemazanyy et al. EMBO Mol Med. 2013;5:
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