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Duong et al Figure 1 A InputsGST-Mdm2 HEGO HE11HE19HE15 HEGO HE11 HE19HE15 B HEGO A/BCEFD 1595 HE11 A/BEFD CEFD HE19 A/BC HE15 C D ER  I GST-Mdm2GST C.

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Presentation on theme: "Duong et al Figure 1 A InputsGST-Mdm2 HEGO HE11HE19HE15 HEGO HE11 HE19HE15 B HEGO A/BCEFD 1595 HE11 A/BEFD CEFD HE19 A/BC HE15 C D ER  I GST-Mdm2GST C."— Presentation transcript:

1 Duong et al Figure 1 A InputsGST-Mdm2 HEGO HE11HE19HE15 HEGO HE11 HE19HE15 B HEGO A/BCEFD 1595 HE11 A/BEFD CEFD HE19 A/BC HE15 C D ER  I GST-Mdm2GST C E2 OHT Luciferase activity Mdm2 ER  Input IP:Mdm Input IP:Mdm * Gal4 Gal-mdm2 + VP16 Gal-mdm2 + ER-VP16 Galmdm2 + VP16+ p53 Gal-mdm2 + ER-VP16+ p53 Gal-GRIP + VP16 Gal-GRIP + ER-VP16 I GST-LBD C E2 Mdm2 GST ER  p53 Mdm Input IP:ER  IP: IgG

2 Duong et al Figure 2 ER  C E2 A Actin MEFWTMEFdKO B C ER   C E2 Controlp53+Mdm2 Mdm D ER  Actin Mdm2 MG Actin ER  ER  Mdm2 w/o Estradiol CHX treatment (hrs) ER  + Mdm2 Actin Mdm2 overexpression - +

3 C A C E2 GST GST-AF2 Input Mdm2 Mdm2  RING Duong et al Figure 3 ER  (HE11) Actin Mdm ER  ER  + Mdm2 WT ER  ER  + Mdm2  RING Mdm2 WT Mdm2  RING Actin C E2 Mdm2 ER  Actin Mdm D Mdm2 - + ER  -Ub ER  IP: ER  IB:  Ub IB:  ER  ER  -Ub B

4 Duong et al Figure 4 C C E2 OHT ICI ControlUV C E2 OHT ICI ER  p53 Actin D A CMG UV ActD Apoptosis (AU) MG132 C E1 E2 E3 DES Control ER  Actin B C E1 E2 E3 DES p53 ER  Actin p53 C E2 ControlMG UVActD ER  p53 Actin

5 A E2 E2+UV Hours of chase ER  levels (% of control) Duong et al Figure 5 C  RITA Control 2h4h8h ER  p53 Actin B E2 +UV E2 CHX treatment (hrs) ER  p53 ER  p53

6 Relative luciferase activity CE2 C p53+Mdm2 MEFdKO B CUV Control E2 A CE2OHTICI MCF7BG1 CE2OHTICI D Duong et al Figure 6 Control Relative luciferase activity C E2 BG1 cells ER  Actin C E2 Control pS2 p53 actin C E2 UV C


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