1. Quick, simple method creation using Venus

2. Venus – Programming Levels
Two levels of programming within Venus
Graphically based tools to quickly create simple methods
Deck Editor
Action Editor
SMART steps
Graphically based tools to quickly create simple methods
Deck Editor
Action Editor
SMART steps
Full access to range of features to build methods that include
Custom user interfaces
Worklists for sample information
Control of Hamilton or 3rd party devices i.e. plate readers
Sample tracking using barcodes
Full access to range of features to build methods that include
Custom user interfaces
Worklists for sample information
Control of Hamilton or 3rd party devices i.e. plate readers
Sample tracking using barcodes

3. Venus – Graphical Programming
Deck Editor
Tool for creating a deck layout
A deck layout is required for each method so venus knows where to pipette or move plates
Either modify an existing layout or create a new one
Venus comes with set of labware installed including carriers and plates
Tool for creating a deck layout
A deck layout is required for each method so venus knows where to pipette or move plates
Either modify an existing layout or create a new one
Venus comes with set of labware installed including carriers and plates
More Details

4. Venus – Graphical Programming
Creating a Deck Layout
Three ways to find labware
Labware Categories – drill down layers
Search – type in labware name
Browse – drill down through Hamilton Labware folder
Three ways to find labware
Labware Categories – drill down layers
Search – type in labware name
Browse – drill down through Hamilton Labware folder
Note – Labware created during your installation can be found in the Hamilton labware folder C:\Program Files\Hamilton\Labware\MyName

5. Venus – Graphical Programming
Creating a Deck Layout
To create a deck
Click on labware and drag labware onto deck
Yellow triangles at corners indicate labware at a snap to fit position
Click on labware and drag labware onto deck
Yellow triangles at corners indicate labware at a snap to fit position

6. Venus – Graphical Programming
Action Editor
Tool for method planning
Drag action from Toolbox to between Start and End blocks to create assay workflow
Insert step into an action to perform pipetting or plate movement
Action Editor
Tool for method planning
Drag action from Toolbox to between Start and End blocks to create assay workflow
Insert step into an action to perform pipetting or plate movement
More Details

7. Venus – Graphical Programming
Action Editor
To insert action
Drag action from Toolbox into action area between Start and End blocks
Drag action from Toolbox into action area between Start and End blocks

8. Venus – Graphical Programming
Action Editor
To insert step
Select step from Insert Step dropdown box
Can only insert step into new action
Select step from Insert Step dropdown box
Can only insert step into new action

9. Venus – Graphical Programming
SMART steps
Wizards to guide you through setting up a pipetting process
Simple (1-1) – transfer from one source well to one destination well
Replica (1-n) - transfer from one source well to multiple destination wells
Pooling (n-1) - transfer from multiple source wells to one destination well
Aliquot – multidispensing from single aspirate
Simple (1-1) – transfer from one source well to one destination well
Replica (1-n) - transfer from one source well to multiple destination wells
Pooling (n-1) - transfer from multiple source wells to one destination well
Aliquot – multidispensing from single aspirate

10. Venus – Graphical Programming
SMART steps
Information required
Where to aspirate from
Where to dispense to
Volume
Tip type
Liquid class
Where to aspirate from
Where to dispense to
Volume
Tip type
Liquid class
More Details

11. Venus – Graphical Programming
SMART steps
Select
Where to aspirate from
Where to dispense to
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.
Where to aspirate from
Where to dispense to

12. Venus – Graphical Programming
SMART steps
Select
Where to aspirate from
Where to dispense to
Volume
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

13. Venus – Graphical Programming
SMART steps
Select
Where to aspirate from
Where to dispense to
Volume
Tip type
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

14. Venus – Graphical Programming
SMART steps
Select
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

15. Venus – Graphical Programming
SMART steps
Select
Where to aspirate from
Where to dispense to
Volume
Tip type
Dispense mode and liquid class
If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

16. Venus – Graphical Programming
Drag and drop programming of SMART steps
Concept enables user to select which wells to aspirate from or dispense to during creation of SMART step
Simply click on wells to be used and drag into appropriate box within SMART step
Concept enables user to select which wells to aspirate from or dispense to during creation of SMART step
Simply click on wells to be used and drag into appropriate box within SMART step
More Details

17. Venus – Graphical Programming
Drag and drop programming
Need to have SMART step open with Deck Editor on sequences tab
Click on wells to be aspirated/dispensed
Need to have SMART step open with Deck Editor on sequences tab
Click on wells to be aspirated/dispensed
Selected wells are Brown

18. Venus – Graphical Programming
Drag and drop programming
Hover mouse over selected wells
Hold down control and drag into appropriate box in SMART step
Hover mouse over selected wells
Hold down control and drag into appropriate box in SMART step
Drag into box
Hover mouse

19. Venus – Graphical Programming
Drag and drop programming
Depending on system configuration settings either
a default name appears in box or
user is asked to enter a name for wells used
Wells used now change colour
Depending on system configuration settings either
a default name appears in box or
user is asked to enter a name for wells used
Wells used now change colour
Default name created
Wells used in aspirate step are now dark blue

20. Venus – Graphical Programming
Quick method creation – putting it all together
Create deck layout
With this approach
Select wells required for each SMART step created.
Two videos with information sheets available demonstrating this approach.
Distribute buffer using Aliquot SMART step
Plate to plate transfer using Simple (1-1) SMART step
With this approach
Select wells required for each SMART step created.
Two videos with information sheets available demonstrating this approach.
Distribute buffer using Aliquot SMART step
Plate to plate transfer using Simple (1-1) SMART step
Insert action block
Plan method in Action Editor
Insert SMART step into Action Block
Fill out SMART step wizard
Test in simulation mode