2 Nuclear Magnetic Resonance NMR is an analytical technique in which magnetic nuclei absorb energy from an applied electromagnetic pulse and radiate the energy backNMR is used for identifying functional groups and imaging
3 Quantum theory Subatomic particles “spin” along their axis. Atoms with paired spins have no net overall spin.Atoms can have a net overall spin if the number of protons or number of protons and neutrons is odd.These atoms have multiple orientationsExamples: 13C, 15N
4 In the absence of an external magnetic field, these orientations have the same energy. In the presence of an external magnetic field, the energy level splitsThe lower energy level contains more nuclei that the higher energy level.
5 Lower energy nuclei can be excited into the higher energy level by electromagnetic radition. The frequency of the radition corresponds to the energy difference between the two nuclei states (transition frequency).After absorption, nuclei relax to lower energy state
6 Chemical ShiftWhen the magnetic field at the nucleis is not equal to the applied magnetic field, the nucleus is sheilded by surrounding electrons.Sheilding: If electrons produce a field opposing the applied magnetic field, the applied field strength must increase in order to reach the transition frueqncyThis results in upfield shiftDesheilding: If the electrons produce a field with the applied magnetic field, the applied field strength must decrease in order to reach the transition frequency.This results in downfield shift
7 Protein StructurePrimary structure is a sequence of amino acids linked by peptide bondsEach amino acid has a unique side chain (R); there are 20 totalPeptide bonds formed by dehydration synthesis (highlighted in red)Chemical interactions of R groups dictate higher structure
8 Protein StructureSecondary structure is the formation of alpha helicies and beta sheetsTertiary structure is the 3D folding of the proteinQuaternary structure is the assembly of multiple protein subunitsMyoglobin from E.Coli(PDB ID: 1CQ2)
9 Protein NMR Series of 2D NMR techniques First is usually 15N-HSQC which results one signal per amino acid residueAdditional peaks from amino acids containing nitrogen in their side chainsBax, A. And Grzesiek, S. Methodological Advances in Protein NMR. Accounts of Chemical Research 36 (1993):(b) HNCO, (c) HNCA, (d) HBHA(CBCACO)NH, and (e) CBCANH experiments. Nuclei for which the chemical shift is measured in the 3D experiment are marked by solid circles. Nuclei involved in the magnetization transfer pathway, but not observed, are marked by open circles. Magnetization transfer in these experiments is marked by curved solid lines, and the direction of the transfer is marked by arrows.Diagram of Interactions study by other NMR techniques including HNCO (b), HNCA (c), HBHA(CBCACO)NH (d) and CBCANH (e)
10 Heteronuclear Single Quantum Coherence (HSQC) Hydrogen nuclei are excited and the energy is transferred to a neighboring 15NThe chemical shift is evolved on the nitrogenEnergy is transferred back to the hydrogen for detection.Mainly show H-N correlationsAmino acids with NH bonds in side chains also have additional peaksInsert image from siteTransfer of Energy betweenan excited hydrogen nucleito a neighboring 15N.
11 Advantages & Disadvantages “Fingerprint”: each protein has a unique patternIdentification of possible problems due to multiple conformation of sampel heterogenityDisadvantagesCan not assign specific peaks to specific atomsNeed further more expensive analysis
12 Tolman, J.R. et al. “Nuclear magnetic dipole interactions in field-oriented protein : Information for structure deermination in solution.” Proc. Natl. Acad. Sci. 92 (1995):Coupling Enhanced 750 Hz HSQC spectrum. Note that there are two peaks on a column for each amide site. Variations can be seen in assigned doublets (veritcal lines). Most variations are small compared to typical 15N line width.
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