1. Double –Stuffed Oreoz Presents:

2. Planting Science By: Jake Schumacher, Shelly Hernandez, Araceli Jimenez, and Ryann Jensen

3. Class Brassica Day 17Class Brassica Day 17 Araceli’s and Jake’s plant.Ryann’s and Shelly’s plant.

4. Our Procedures: Our group, The Double-Stuffed Oreoz started by planting our Brassica plants. We planted the plants in plastic film canisters. We poked a hole at the end of each canister, and stuck string or black material through the holes. We cut 4 holes in a lid and stuck one canisters in each hole. We stuck the lid on top of it’s container. The container was filled with water. The string or material would soak up the water, and make the soil wet. We then put 5 milliliters of water on top of each plant. After we watered our Brassica plants, we put a blue fertilizing solution on the them. After we took care of the Brassica plants, we put them in the light boxes, which acted as the sun. The next day we took the Brassica plants out of the light boxes to water them. We then took pictures of the Brassica plants, and posted them on our webpage, so the scientist could look at them, and give us advice. We would also measure how tall our plants got. This was a routine we did everyday. Every Friday we put the blue fertilizing solution on the Brassica plants. Once the leaves and flowers started growing on the plants, we stopped putting the blue fertilizing solution on them. The flowers started to open and pods were being produced. Once this started happening, Mrs. McLean supplied us with dead bees, which we separated the butts from the bodies and hot glued the butts to toothpicks. Each member of our group got their own bee stick (toothpicks with the bee butts on them). We rubbed our bee sticks on the newly produced pods to pollinate them. The next day we switched our bee stick with another member of our group’s bee stick. Araceli switched with Ryann and Jake switched with Shelly. We then rubbed the second bee stick on our pods. We did this so the plants would reproduce and make seeds. We knew the bee sticks were working, if the bee’s coat turned a yellow color. We pollinated during Day 19 and through Day 22. We eventually stopped top-watering the plants. The plants would get their water supply from the containers we put them in. We left the Brassica plants alone until they dried out. We then took the pods off of each plant and opened them to get our seeds out. After our sees were out we put them in a tiny envelope. We then reported to the scientist how many seeds and pods our plants produced.

5. Mutant Brassica Day 20 Araceli’s and Jake’s plant. Shelly’s and Ryann’s plant.

6. Double-Stuffed Oreoz Journals Araceli- After drying my plants out, I opened up my pods. My mutant plant only produced 30 seeds and my class produced 13 seeds. My mutant plant had 9 pods, and my class plant had 5 pods. Now we are using petri dishes to germinate our seeds. We cut and doubled paper towels and soaked them in water. Then we put 16 seeds in each petri dish. Ryann- After I dried my plants out, I opened my pods, but my mutant plant only produced one seed and my class plant only produced four seeds. I had about ten pods on each plant, but they didn’t have seeds in them. I don’t know why my plant did this, because I did everything the same as my group. My pods just didn’t produce right. After we got our seeds, we put our seeds in petri dishes. We cut two circles out of paper towels, and put them together, then put them at the bottom of the petri dish. We soaked the paper towels, so the seeds would stick to them, and not flop around. We did this so our seeds would germinate. Because I didn’t have many seeds, I had to use about fifteen of Araceli’s mutant seeds, and twelve of Shelly’s class seeds. Jake- After opening my pods, I found that I had twenty-three seeds in total, ten class and thirteen mutant plants. We cut two circles out of paper towels and soaked them in water then we placed them in petri dishes with our seeds on top. We did this for both the class and mutant seeds then we put them in a container part way full of water to help germinate the seeds. April- Once I had opened my pods, I had found quite a bit of seeds in them. In all I had 43 seeds in all, 10 mutant plants and 33 class plants. Once I had opened them I cut out a couple of circles out of paper towels. I place them in a petri dish after I had soaked the paper towels. I placed the seeds inside the petri dish and then placed it in a container partially filled with water to help germinate them.

7. Class and Mutant Brassica Charts Jake17.08 Shelly6.58 Araceli10.46 Ryann11.09 Jake4.07 Shelly6.12 Araceli3.47 Ryann3.05

8. Class Brassica Day 26 Jake’s and Araceli’s plant. Ryann’s and Shelly’s plant.

9. Research Question: Does the height of the plant determine how man leaves and seeds the plant will produce? No, the height of the plant does not determine the outcome of how many seeds and leaves the plant will produce. Jake and Ryann had the taller of the 4 class Brassica plants, and Jake’s plant produced more leaves than Ryann’s, Shelly’s, and Araceli’s plants, but Shelly’s plant produced the most seeds. Jake and Shelly had the taller mutant Brassica plants, and Ryann had more leaves than the other 4 mutant Brassica plants, but Araceli’s plant produced the most seeds. So in conclusion, it didn’t matter how tall the plant was, just how well the plant responded to the way we took care of them. That’s what determined the amount of leaves and seeds produced.

10. The Petri Dishes: We are using the petri dishes to germinate our seeds. We each got 2 petri dishes, 1 for the mutant seeds and 1 for the class seeds. Then we got 4 paper towels and put them together. We took 1 petri dish and traced 8 circles on 2 of the paper towels. Then we cut the circles out, so all together we had 16 circles. We soaked the paper towels, and put 2 circles in each dish. We put the seeds on the paper towels, and put the lid on the dishes. We then got 2 plastic containers, and filled the containers with 1-2 inches of water. We put the mutant seed dishes in 1 container sideways. We put them in sideways so that the water will touch the paper towels, to keep them wet, but not drowning the seeds. We did the same thing with the class seed dishes. We then put the containers of dishes into the light box. The next day we took the dishes out of the containers, and noticed that the seeds had little plant stems coming out of the seeds. Some of the seeds were purple and others were green. We had more purple mutant seeds and more green class seeds. The Brassica plants that produced seeds that had purple stems coming from them, were purple. Same with the green Brassica plants.

11. ‘Oreoz Mutant Seeds in the Petri Dishes Shelly’s and Ryann’s Jake’s and Araceli’s

12. ‘Oreoz Class seeds in the Petri Dishes Ryann’s and Shelly’s Jake’s and Araceli’s

13. Thank you for watching our Power Point Presentation!!! By: Ryann Jensen, Araceli Jimenez, Jake Schumacher, and Shelly Hernandez