1. BIO409/509 Cell and Molecular Biology

2. SECOND Methods paper assignment due Wed., 4/20 (you don’t do this assignment if you are in the 4H STEM Ambassador Program) Assignment: Choose a SECOND method in Molecular Biology or Cell Biology and write 2-3 page paper about the following: 1)Describe the steps done in the method 2)Explain the “highlight” steps: What are the key “tricks” in the method. 3)Describe two different applications of the method to help demonstrate how the method is valuable. On an extra page, include a bibliography with any resources you used: Our textbook, web sites, personal communications, journal articles, etc.

3. BIO409/509 Cell and Molecular Biology We will skip Chapter 7 DNA Replication and never cover it. Finish Chapter 8 RNA Synthesis on on to Chapter 9 Protein Synthesis. That’s the end of Part II. We’ll start talking about Chapter 10 The Nucleus this week.

4. RNA Synthesis and Processing 8

7. Figure 8.31 Histone acetylation (Part 2)

8. Figure 8.37 DNA methylation

9. Figure 8.38 Maintenance of methylation patterns

11. Each step in gene expression has the potential for being regulated if it is the rate limiting step.

12. Regulation of Protein Function Cells can regulate the amounts and the activities of their proteins. Three mechanisms: Regulation by small molecules Phosphorylation and other modifications Protein-protein interactions

13. Figure 8.44 Processing of eukaryotic messenger RNAs (Part 1)

14. Figure 8.47 Splicing of pre-mRNA

15. Figure 8.48 Assembly of the spliceosome (Part 2)

16. Figure 8.52 Alternative splicing in Drosophila sex determination

17. Figure 8.55 mRNA degradation

18. Protein Synthesis, Processing, and Regulation 9

19. 9 Protein Synthesis, Processing, and Regulation Translation of mRNA Protein Folding and Processing Regulation of Protein Function Protein Degradation

20. Figure 9.9 Overview of translation

21. Figure 9.7 Prokaryotic and eukaryotic mRNAs

22. Figure 9.8 Signals for translation initiation

23. Table 9.1 Translation Factors

24. Figure 9.11 Initiation of translation in eukaryotic cells (Part 1)

25. Figure 9.17 Translational regulation of ferritin

26. Figure 9.22 Regulation of eIF4E

27. Translation of mRNA RNA interference is mediated by: Small interfering RNAs (siRNAs)— produced from double-stranded RNAs by the nuclease Dicer. MicroRNAs (miRNAs)—transcribed by RNA polymerase II, then cleaved by nucleases Drosha and Dicer.

28. Figure 9.13 Elongation stage of translation (Part 1)

29. Figure 9.13 Elongation stage of translation (Part 3)