Presentation on theme: "Unit #3 Schedule: Last Class: – Sanger Sequencing – Central Dogma Overview – Mutation Today: – Homework 5 – StudyNotes 8a Due – Transcription, RNA Processing,"— Presentation transcript:
Unit #3 Schedule: Last Class: – Sanger Sequencing – Central Dogma Overview – Mutation Today: – Homework 5 – StudyNotes 8a Due – Transcription, RNA Processing, Translation Central Dogma Sculpting (Apr 2) – StudyNotes 8b Due Regulation of Gene Expression + Trivia (Apr 4) – StudyNotes 9 Due Tutorial (Apr 5) Review (Apr 9) – Homework 6 Due EXAM 3 (Apr 11)
Unpacking Central Dogma: Transcription
Learning Objectives: By the end of today’s class you should be able to: 1.Describe transcription. 2.Compare and contrast eukaryotic + prokaryotic transcription. 3.Explain mRNA processing in eukaryotes. 4.Describe the general structure of a tRNA molecule. 5.Discuss the three stages of translation.
Fig. 17-7a-4 Promoter Transcription unit DNA Start point RNA polymerase Initiation RNA transcript 5 5 Unwound DNA Template strand of DNA 2 Elongation Rewound DNA RNA transcript 3 Termination Completed RNA transcript
Fig Transcription Termination in Eukaryotes: Transcribes the sequence AAUAAA Signals for the RNA transcript to be cleaved.
Transcription Termination Sequences In Prokaryotes, transcription stops by way of as transcribed terminator. This is part of the transcription sequence that signals RNA polymerase to detach. (Hairpin theory) In Eukaryotes, transcription stops when a polyadenylation signal AAUAAA occurs. Proteins then cleave the RNA transcript off.