1. Lab Exercise 2 Culture Media

2. General Purpose Media: Nutrient Broth and Nutrient Agar

3. Enriched Media

4. Selective Media: Mycological agar

5. Glucose Fermentation a. Neutral pH uninoculated tube of glucose broth with phenol red pH indicator. b. Fermentation of glucose and acid production (color change from red to yellow) (acidic pH) c. Organism growth, but negative for acid production (alkaline pH) Differential Media a bc

6. left: no lactose fermentation right: lactose fermentation Differential Media: MacConkey Agar

7. Gel media (in-between liquid broth and solid agar) is used to test for motility Spreading out indicates motility

8. Sterilization by autoclaving: 15-20 psi at 121°C for15-20 minutes

9. Bunsen burner flame

10. Aseptically pouring agar plates

11. Agar plates are stored upside down to prevent condensation.

12. All labeling is done on the bottom of the agar plate 1.Initials 2. Date (mm/dd/yy) 3. Code # or letter

13. Environmental sampling

14. Typical environmental sampling results

15. Microbes in the Environment. This plate was inoculated with 500 L of indoor air. Approximately how many colonies are on the plate? How many different bacterial species are here? 1 2 3 4 5 6 7 8

16. Microbes in the Environment. This plate was inoculated with a cotton swab that was wiped over a shower drain. Fungi usually produce large, "fuzzy" colonies (marked F). Approximately how many colonies are on the plate? How many are bacterial colonies? How many different bacterial species are here? F