1. End Show Slide 1 of 32 Copyright Pearson Prentice Hall Biology

2. End Show Slide 2 of 32 Copyright Pearson Prentice Hall 13-2 Manipulating DNA

3. End Show 13-2 Manipulating DNA Slide 3 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology How do scientists make changes to DNA? The Tools of Molecular Biology

4. End Show 13-2 Manipulating DNA Slide 4 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology Scientists use different techniques to: extract DNA from cells cut DNA into smaller pieces identify the sequence of bases in a DNA molecule make unlimited copies of DNA

5. End Show 13-2 Manipulating DNA Slide 5 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology In genetic engineering, biologists make changes in the DNA code of a living organism.

6. End Show 13-2 Manipulating DNA Slide 6 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology DNA Extraction DNA can be extracted from most cells by a simple chemical procedure. The cells are opened and the DNA is separated from the other cell parts.

7. End Show 13-2 Manipulating DNA Slide 7 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology Cutting DNA Most DNA molecules are too large to be analyzed, so biologists cut them into smaller fragments using restriction enzymes. Which type of molecule is an enzyme?

8. End Show 13-2 Manipulating DNA Slide 8 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology Each restriction enzyme cuts DNA at a specific sequence of nucleotides. Recognition sequences DNA sequence Restriction enzyme EcoR I cuts the DNA into fragments Sticky end

9. End Show 13-2 Manipulating DNA Slide 9 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology Separating DNA In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel.

10. End Show 13-2 Manipulating DNA Slide 10 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology DNA plus restriction enzyme Mixture of DNA fragments Gel Power source Gel Electrophoresis Longer fragments Shorter fragments

11. End Show 13-2 Manipulating DNA Slide 11 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology First, restriction enzymes cut DNA into fragments. The DNA fragments are poured into wells on a gel. DNA plus restriction enzyme Mixture of DNA fragments Gel Gel Electrophoresis

12. End Show 13-2 Manipulating DNA Slide 12 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology An electric voltage is applied to the gel. The smaller the DNA fragment, the faster and farther it will move across the gel. Power source

13. End Show 13-2 Manipulating DNA Slide 13 of 32 Copyright Pearson Prentice Hall The Tools of Molecular Biology Longer fragments Shorter fragments Gel Electrophoresis Power source

14. End Show 13-2 Manipulating DNA Slide 14 of 32 DNA Fingerprinting Use In Crime Scene Investigations To interpret the results of a DNA fingerprint, match the banding pattern results from the gel electrophoresis. Who is guilty in the above example? Copyright Pearson Prentice Hall

15. End Show 13-2 Manipulating DNA Slide 15 of 32 DNA Fingerprinting Use In Crime Scene Investigations Copyright Pearson Prentice Hall

16. End Show 13-2 Manipulating DNA Slide 16 of 32 DNA Fingerprinting Use In Paternity Testing A child’s DNA will have a similar banding pattern of that of the mother’s and the father’s but not identical of either. Copyright Pearson Prentice Hall

17. End Show 13-2 Manipulating DNA Slide 17 of 32 Copyright Pearson Prentice Hall Using the DNA Sequence Making Copies Polymerase chain reaction (PCR) is a technique that allows biologists to make copies of genes. Small amounts of DNA can be multiplied making it easier to analyze. Made possible by an enzyme found in a bacterium living in hot springs in Yellow Stone National Park.

18. End Show 13-2 Manipulating DNA Slide 18 of 32 Copyright Pearson Prentice Hall Using the DNA Sequence DNA heated to separate strands PCR cycles DNA copies 12345 etc. 124816 etc. Polymerase Chain Reaction (PCR) DNA polymerase adds complementary strand DNA fragment to be copied

19. End Show - or - Continue to: Click to Launch: Slide 19 of 32 Copyright Pearson Prentice Hall 13-2

20. End Show Slide 20 of 32 Copyright Pearson Prentice Hall 13-2 Restriction enzymes are used to a.extract DNA. b.cut DNA. c.separate DNA. d.replicate DNA.

21. End Show Slide 21 of 32 Copyright Pearson Prentice Hall 13-2 During gel electrophoresis, the smaller the DNA fragment is, the a.more slowly it moves. b.heavier it is. c.more quickly it moves. d.darker it stains.

22. End Show Slide 22 of 32 Copyright Pearson Prentice Hall 13-2 The DNA polymerase enzyme found in bacteria living in the hot springs of Yellowstone National Park illustrates a.genetic engineering. b.the importance of biodiversity to biotechnology. c.the polymerase chain reaction. d.selective breeding.

23. End Show Slide 23 of 32 Copyright Pearson Prentice Hall 13-2 A particular restriction enzyme is used to a.cut up DNA in random locations. b.cut DNA at a specific nucleotide sequence. c.extract DNA from cells. d.separate negatively charged DNA molecules.

24. End Show Slide 24 of 32 Copyright Pearson Prentice Hall 13-2 During gel electrophoresis, DNA fragments become separated because a.multiple copies of DNA are made. b.recombinant DNA is formed. c.DNA molecules are negatively charged. d.smaller DNA molecules move faster than larger fragments.

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