Presentation on theme: "Aim: How do scientists identify people using DNA Fingerprinting?"— Presentation transcript:
1 Aim: How do scientists identify people using DNA Fingerprinting?
2 LO: SWBAT explain how scientists identify people using DNA fingerprinting DN: Why do we fingerprint people? What is it useful for?HW: read pp , page 339 #18-19Fri: Review Book- RespirationQuarterly- Next Wed/Thurs
4 Why is DNA used to identify people? People have different DNA base sequences, therefore each individual will have a different DNA fingerprint.
5 Genetic Markers: ATTGCCTAGG - Individual 1 ATTGCCATGG - Individual 2 particular stretches of ____ that are _________ among individuals.DNAvariableOur __________ regions are highly _________ from one individual to another and serve as good ________________ to be used for DNA fingerprinting.noncodingvariablegenetic markers
6 How do scientists make a DNA Fingerprint? Investigators need to find some ____ of the perpetrator.DNAWhere can the investigators find the DNA?BloodHairNailsSkin
7 Only a small amount of DNA is needed! Polymerase Chain Reaction: PCRThe sample of DNA is amplified (___________)multiplied
9 – + Gel Electrophoresis Amplified DNA is cut with specific __________________.2) DNA segments are loaded into wells in a porous ____.3) Electric current is passed through the gel making one side of the gel __ charged and one side __ charged.restriction enzymesgel–+
13 Gel Electrophoresis6) The gel is then ________ so we can see the DNA ________ patterns.stainedbandingResult: The smaller segments of DNA are found closer to the _________ end than the larger segments.positive
15 **Separates DNA segments according to _____.SIZE
16 - + Gel Electrophoresis __ __ __ __ __ __ __ __ __ __ DNA Suspect A Suspect B Suspect Cfrom CS__ __ ______ ____ __ __ __+
17 DNA Fingerprint Questions: What is the charge of DNA?Where would we find the smallest DNA fragments, closer to the + end or the – end?Why do we get different banding patterns for each individual?4) Which suspect was at the crime scene?