1. The Genomics: GTL Program Environmental Remediation Sciences Program Spring Workshop April 3, 2006

2. Genomics: GTL — A Multidisciplinary Program Finding Solutions to DOE Mission Challenges Inclusion “Mesosome” Nucleoid Ribosome bodies Capsule Genome Sequencing Analytical Chemistry Computational Biology/Modeling Structural Genomics High Resolution Imaging/Microscopy Carbon Sequestration Toxic Waste Remediation Energy Production Flagellum Surface Cell Wall Periplasmic Plasma proteins space membrane Microbial Ecology Microbiology

3. Genomics: GTL – A Systems Biology Research Program Identification, subcellular location, and dynamics of molecular machines Regulation of gene expression in individual cells SubcellularCellular Ecosystems Who is expressing what, when, where, and under what conditions? How do they work together? From Molecules to Cells to Ecosystems

4. GTL Program Goals Achieve a predictive understanding of microbial function through systems biology to enable progress toward biotechnology solutions for DOE missions. Specifically: –Understand the genes, proteins and protein machines that perform a cell’s functions –Understand the regulatory networks that control complex cellular processes –Understand how diverse microbial species interact in communities to respond to and alter their environments Use this understanding to: –Develop bio-based fuels as a major secure energy source –Develop biological solutions for DOE’s environmental remediation problems –Explain how microbial systems influence the global carbon cycle