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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Actin, alpha cardiac muscle 1ACTC_HUMAN42 kDa2 Actin, cytoplasmic 1ACTB_HUMAN42 kDa15 Actin-related protein 2ARP2_HUMAN45 kDa3 Actin-related protein 2/3 complex subunit 1BARC1B_HUMAN41 kDa1 Actin-related protein 3ARP3_HUMAN47 kDa2 Activated RNA polymerase II transcriptional coactivator p15TCP4_HUMAN14 kDa1 Acyl-CoA-binding proteinACBP_HUMAN10 kDa3 Adenylyl cyclase-associated protein 1CAP1_HUMAN52 kDa1 Alpha-1-acid glycoprotein 1A1AG1_HUMAN24 kDa7 Alpha-1-acid glycoprotein 2A1AG2_HUMAN24 kDa2 Alpha-1-antichymotrypsinAACT_HUMAN48 kDa1 Alpha-1-antitrypsinA1AT_HUMAN47 kDa13 Alpha-1B-glycoproteinA1BG_HUMAN54 kDa3 Alpha-2-HS-glycoproteinFETUA_HUMAN39 kDa3 Alpha-2-macroglobulinA2MG_HUMAN163 kDa12 Alpha-2-macroglobulin-like protein 1A2ML1_HUMAN161 kDa1 Alpha-actinin-1ACTN1_HUMAN103 kDa14 Alpha-actinin-4ACTN4_HUMAN105 kDa3 Alpha-amylase 1AMY1_HUMAN58 kDa22 Alpha-crystallin B chainCRYAB_HUMAN20 kDa1 Alpha-enolaseENOA_HUMAN47 kDa14 AmidophosphoribosyltransferasePUR1_HUMAN57 kDa1 Annexin A1ANXA1_HUMAN39 kDa11 Annexin A2ANXA2_HUMAN39 kDa2 Annexin A3ANXA3_HUMAN36 kDa5 Annexin A5ANXA5_HUMAN36 kDa2 Annexin A6ANXA6_HUMAN76 kDa2 AntileukoproteinaseSLPI_HUMAN14 kDa2 Antithrombin-IIIANT3_HUMAN53 kDa2 Apolipoprotein A-IAPOA1_HUMAN31 kDa1[1] Apolipoprotein A-IIAPOA2_HUMAN11 kDa1 Arginase-1ARGI1_HUMAN35 kDa2 ATP synthase subunit alpha, mitochondrialATPA_HUMAN60 kDa2 ATP synthase subunit beta, mitochondrialATPB_HUMAN57 kDa1 AzurocidinCAP7_HUMAN27 kDa4 Beta-2-glycoprotein 1APOH_HUMAN38 kDa5 Beta-2-microglobulinB2MG_HUMAN14 kDa1 Brain acid soluble protein 1BASP1_HUMAN23 kDa3 C4b-binding protein alpha chainC4BPA_HUMAN67 kDa4 Calcineurin-like phosphoesterase domain-containing protein 1CPPED_HUMAN36 kDa1 CalmodulinCALM_HUMAN17 kDa2 Calmodulin-like protein 3CALL3_HUMAN17 kDa1 CalnexinCALX_HUMAN68 kDa1 CalpastatinICAL_HUMAN77 kDa1 CalreticulinCALR_HUMAN48 kDa1 Carcinoembryonic antigen-related cell adhesion molecule 8CEAM8_HUMAN38 kDa2 CatalaseCATA_HUMAN60 kDa11 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease. Suppl Table 1. LC-MS/MS identification of proteins in GCF from healthy subjects
![Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Actin, alpha cardiac muscle 1ACTC_HUMAN42 kDa2 Actin, cytoplasmic 1ACTB_HUMAN42 kDa15 Actin-related protein 2ARP2_HUMAN45 kDa3 Actin-related protein 2/3 complex subunit 1BARC1B_HUMAN41 kDa1 Actin-related protein 3ARP3_HUMAN47 kDa2 Activated RNA polymerase II transcriptional coactivator p15TCP4_HUMAN14 kDa1 Acyl-CoA-binding proteinACBP_HUMAN10 kDa3 Adenylyl cyclase-associated protein 1CAP1_HUMAN52 kDa1 Alpha-1-acid glycoprotein 1A1AG1_HUMAN24 kDa7 Alpha-1-acid glycoprotein 2A1AG2_HUMAN24 kDa2 Alpha-1-antichymotrypsinAACT_HUMAN48 kDa1 Alpha-1-antitrypsinA1AT_HUMAN47 kDa13 Alpha-1B-glycoproteinA1BG_HUMAN54 kDa3 Alpha-2-HS-glycoproteinFETUA_HUMAN39 kDa3 Alpha-2-macroglobulinA2MG_HUMAN163 kDa12 Alpha-2-macroglobulin-like protein 1A2ML1_HUMAN161 kDa1 Alpha-actinin-1ACTN1_HUMAN103 kDa14 Alpha-actinin-4ACTN4_HUMAN105 kDa3 Alpha-amylase 1AMY1_HUMAN58 kDa22 Alpha-crystallin B chainCRYAB_HUMAN20 kDa1 Alpha-enolaseENOA_HUMAN47 kDa14 AmidophosphoribosyltransferasePUR1_HUMAN57 kDa1 Annexin A1ANXA1_HUMAN39 kDa11 Annexin A2ANXA2_HUMAN39 kDa2 Annexin A3ANXA3_HUMAN36 kDa5 Annexin A5ANXA5_HUMAN36 kDa2 Annexin A6ANXA6_HUMAN76 kDa2 AntileukoproteinaseSLPI_HUMAN14 kDa2 Antithrombin-IIIANT3_HUMAN53 kDa2 Apolipoprotein A-IAPOA1_HUMAN31 kDa1[1] Apolipoprotein A-IIAPOA2_HUMAN11 kDa1 Arginase-1ARGI1_HUMAN35 kDa2 ATP synthase subunit alpha, mitochondrialATPA_HUMAN60 kDa2 ATP synthase subunit beta, mitochondrialATPB_HUMAN57 kDa1 AzurocidinCAP7_HUMAN27 kDa4 Beta-2-glycoprotein 1APOH_HUMAN38 kDa5 Beta-2-microglobulinB2MG_HUMAN14 kDa1 Brain acid soluble protein 1BASP1_HUMAN23 kDa3 C4b-binding protein alpha chainC4BPA_HUMAN67 kDa4 Calcineurin-like phosphoesterase domain-containing protein 1CPPED_HUMAN36 kDa1 CalmodulinCALM_HUMAN17 kDa2 Calmodulin-like protein 3CALL3_HUMAN17 kDa1 CalnexinCALX_HUMAN68 kDa1 CalpastatinICAL_HUMAN77 kDa1 CalreticulinCALR_HUMAN48 kDa1 Carcinoembryonic antigen-related cell adhesion molecule 8CEAM8_HUMAN38 kDa2 CatalaseCATA_HUMAN60 kDa11 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS.](http://images.slideplayer.com/31/9770369/slides/slide_1.jpg "Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease. Suppl Table 1. LC-MS/MS identification of proteins in GCF from healthy subjects.")
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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Cathelicidin antimicrobial peptideCAMP_HUMAN19 kDa5[2] Cathepsin GCATG_HUMAN29 kDa3[3] Cathepsin SCATS_HUMAN37 kDa1 CD177 antigenCD177_HUMAN46 kDa1 Cellular retinoic acid-binding protein 2RABP2_HUMAN16 kDa1 CeruloplasminCERU_HUMAN122 kDa6 Chitinase-3-like protein 1CH3L1_HUMAN43 kDa2 Chitotriosidase-1CHIT1_HUMAN52 kDa1 Chloride intracellular channel protein 1CLIC1_HUMAN27 kDa2 Cofilin-1COF1_HUMAN19 kDa4 Complement C3CO3_HUMAN187 kDa14 Complement C4-ACO4A_HUMAN193 kDa1 Complement component C6CO6_HUMAN105 kDa1 Complement factor HCFAH_HUMAN139 kDa3 Core histone macro-H2A.1H2AY_HUMAN40 kDa1 Cornifin-ASPR1A_HUMAN10 kDa1 Cornifin-BSPR1B_HUMAN10 kDa2 CornulinCRNN_HUMAN54 kDa6 Coronin-1ACOR1A_HUMAN51 kDa4 Cystatin-ACYTA_HUMAN11 kDa6 Cystatin-BCYTB_HUMAN11 kDa3 Cystatin-CCYTC_HUMAN16 kDa1[4] Cystatin-SCYTS_HUMAN16 kDa7 Cystatin-SACYTT_HUMAN16 kDa3 Cystatin-SNCYTN_HUMAN16 kDa4 Cysteine-rich secretory protein 3CRIS3_HUMAN28 kDa3 Cytidine deaminaseCDD_HUMAN16 kDa1 Cytochrome b-245 heavy chainCY24B_HUMAN65 kDa1 Deleted in malignant brain tumors 1 proteinDMBT1_HUMAN261 kDa3 DermcidinDCD_HUMAN11 kDa3 Desmocollin-2DSC2_HUMAN100 kDa1 DesmoplakinDESP_HUMAN332 kDa4 Drebrin-like proteinDBNL_HUMAN48 kDa1 EF-hand domain-containing protein D2EFHD2_HUMAN27 kDa6 Elongation factor 1-alpha 1EF1A1_HUMAN50 kDa2 Elongation factor 1-gammaEF1G_HUMAN50 kDa2 Elongation factor 2EF2_HUMAN95 kDa2 Erythrocyte band 7 integral membrane proteinSTOM_HUMAN32 kDa1 Eukaryotic initiation factor 4A-IIF4A1_HUMAN46 kDa1 Extracellular matrix protein 1ECM1_HUMAN61 kDa1 EzrinEZRI_HUMAN69 kDa3 F-actin-capping protein subunit alpha-1CAZA1_HUMAN33 kDa1 Fatty acid-binding protein, epidermalFABP5_HUMAN15 kDa5 Fibrinogen alpha chainFIBA_HUMAN95 kDa5 Fibrinogen beta chainFIBB_HUMAN56 kDa11 Fibrinogen gamma chainFIBG_HUMAN52 kDa9 FibronectinFINC_HUMAN263 kDa4 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
![Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Cathelicidin antimicrobial peptideCAMP_HUMAN19 kDa5[2] Cathepsin GCATG_HUMAN29 kDa3[3] Cathepsin SCATS_HUMAN37 kDa1 CD177 antigenCD177_HUMAN46 kDa1 Cellular retinoic acid-binding protein 2RABP2_HUMAN16 kDa1 CeruloplasminCERU_HUMAN122 kDa6 Chitinase-3-like protein 1CH3L1_HUMAN43 kDa2 Chitotriosidase-1CHIT1_HUMAN52 kDa1 Chloride intracellular channel protein 1CLIC1_HUMAN27 kDa2 Cofilin-1COF1_HUMAN19 kDa4 Complement C3CO3_HUMAN187 kDa14 Complement C4-ACO4A_HUMAN193 kDa1 Complement component C6CO6_HUMAN105 kDa1 Complement factor HCFAH_HUMAN139 kDa3 Core histone macro-H2A.1H2AY_HUMAN40 kDa1 Cornifin-ASPR1A_HUMAN10 kDa1 Cornifin-BSPR1B_HUMAN10 kDa2 CornulinCRNN_HUMAN54 kDa6 Coronin-1ACOR1A_HUMAN51 kDa4 Cystatin-ACYTA_HUMAN11 kDa6 Cystatin-BCYTB_HUMAN11 kDa3 Cystatin-CCYTC_HUMAN16 kDa1[4] Cystatin-SCYTS_HUMAN16 kDa7 Cystatin-SACYTT_HUMAN16 kDa3 Cystatin-SNCYTN_HUMAN16 kDa4 Cysteine-rich secretory protein 3CRIS3_HUMAN28 kDa3 Cytidine deaminaseCDD_HUMAN16 kDa1 Cytochrome b-245 heavy chainCY24B_HUMAN65 kDa1 Deleted in malignant brain tumors 1 proteinDMBT1_HUMAN261 kDa3 DermcidinDCD_HUMAN11 kDa3 Desmocollin-2DSC2_HUMAN100 kDa1 DesmoplakinDESP_HUMAN332 kDa4 Drebrin-like proteinDBNL_HUMAN48 kDa1 EF-hand domain-containing protein D2EFHD2_HUMAN27 kDa6 Elongation factor 1-alpha 1EF1A1_HUMAN50 kDa2 Elongation factor 1-gammaEF1G_HUMAN50 kDa2 Elongation factor 2EF2_HUMAN95 kDa2 Erythrocyte band 7 integral membrane proteinSTOM_HUMAN32 kDa1 Eukaryotic initiation factor 4A-IIF4A1_HUMAN46 kDa1 Extracellular matrix protein 1ECM1_HUMAN61 kDa1 EzrinEZRI_HUMAN69 kDa3 F-actin-capping protein subunit alpha-1CAZA1_HUMAN33 kDa1 Fatty acid-binding protein, epidermalFABP5_HUMAN15 kDa5 Fibrinogen alpha chainFIBA_HUMAN95 kDa5 Fibrinogen beta chainFIBB_HUMAN56 kDa11 Fibrinogen gamma chainFIBG_HUMAN52 kDa9 FibronectinFINC_HUMAN263 kDa4 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS.](http://images.slideplayer.com/31/9770369/slides/slide_2.jpg "Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease..")
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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Filaggrin-2FILA2_HUMAN248 kDa1 Filamin-AFLNA_HUMAN281 kDa12 Flotillin-1FLOT1_HUMAN47 kDa1 Fructose-bisphosphate aldolase AALDOA_HUMAN39 kDa3 Galectin-7LEG7_HUMAN15 kDa1 Gamma-glutamyl hydrolaseGGH_HUMAN36 kDa1 GelsolinGELS_HUMAN86 kDa10 Glucose-6-phosphate 1-dehydrogenaseG6PD_HUMAN59 kDa1 Glucose-6-phosphate isomeraseG6PI_HUMAN63 kDa10 Glucosidase 2 subunit betaGLU2B_HUMAN59 kDa1 Glutathione S-transferase PGSTP1_HUMAN23 kDa2 Glyceraldehyde-3-phosphate dehydrogenaseG3P_HUMAN36 kDa8 Glycogen phosphorylase, liver formPYGL_HUMAN97 kDa1 GranulinsGRN_HUMAN64 kDa3 GTP-binding nuclear protein RanRAN_HUMAN24 kDa1 Guanine nucleotide-binding protein G(i) subunit alpha-2GNAI2_HUMAN40 kDa1 HaptoglobinHPT_HUMAN45 kDa4 Heat shock 70 kDa protein 1A/1BHSP71_HUMAN70 kDa14 Heat shock cognate 71 kDa proteinHSP7C_HUMAN71 kDa9 Heat shock protein beta-1HSPB1_HUMAN23 kDa3 Heat shock protein HSP 90-alphaHS90A_HUMAN85 kDa1 Heat shock protein HSP 90-betaHS90B_HUMAN83 kDa3 Hematopoietic lineage cell-specific proteinHCLS1_HUMAN54 kDa3 Heme-binding protein 2HEBP2_HUMAN23 kDa3 Hemoglobin subunit alphaHBA_HUMAN15 kDa5 Hemoglobin subunit betaHBB_HUMAN16 kDa4 HemopexinHEMO_HUMAN52 kDa8 Heterogeneous nuclear ribonucleoprotein D0HNRPD_HUMAN38 kDa1 Heterogeneous nuclear ribonucleoproteins A2/B1ROA2_HUMAN37 kDa2 Heterogeneous nuclear ribonucleoproteins C1/C2HNRPC_HUMAN34 kDa1 High mobility group protein B2HMGB2_HUMAN24 kDa2 Histidine-rich glycoproteinHRG_HUMAN60 kDa5 Histone H1.4H14_HUMAN22 kDa5 Histone H1.5H15_HUMAN23 kDa5 Histone H2A type 1-B/EH2A1B_HUMAN14 kDa3 Histone H2A type 1-DH2A1D_HUMAN14 kDa1 Histone H2B type 1-C/E/F/G/IH2B1C_HUMAN14 kDa2 Histone H3.1H31_HUMAN15 kDa2 Histone H4H4_HUMAN11 kDa7 HornerinHORN_HUMAN282 kDa1 Ig alpha-1 chain C regionIGHA1_HUMAN38 kDa11 Ig alpha-2 chain C regionIGHA2_HUMAN37 kDa2 Ig gamma-1 chain C regionIGHG1_HUMAN36 kDa15 Ig gamma-2 chain C regionIGHG2_HUMAN36 kDa6 Ig gamma-3 chain C regionIGHG3_HUMAN41 kDa5 Ig gamma-4 chain C regionIGHG4_HUMAN36 kDa3 Ig heavy chain V-I region HG3HV102_HUMAN13 kDa1 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
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Identified ProteinsAccession Number Molecular Weight MS/MS(unique)References* Ig heavy chain V-III region BROHV305_HUMAN13 kDa2 Ig heavy chain V-III region GALHV320_HUMAN13 kDa1 Ig heavy chain V-III region TILHV304_HUMAN12 kDa2 Ig heavy chain V-III region TURHV318_HUMAN12 kDa1 Ig heavy chain V-III region VH26HV303_HUMAN13 kDa1 Ig kappa chain C regionIGKC_HUMAN12 kDa9 Ig kappa chain V-I region AGKV101_HUMAN12 kDa1 Ig kappa chain V-I region EUKV106_HUMAN12 kDa2 Ig kappa chain V-I region LayKV113_HUMAN12 kDa1 Ig kappa chain V-II region CumKV201_HUMAN13 kDa1 Ig kappa chain V-III region SIEKV302_HUMAN12 kDa3 Ig kappa chain V-III region VG (Fragment)KV309_HUMAN13 kDa1 Ig kappa chain V-IV region LenKV402_HUMAN13 kDa2 Ig lambda chain V-I region HALV102_HUMAN12 kDa1 Ig lambda chain V-III region LOILV302_HUMAN12 kDa1 Ig lambda chain V-III region SHLV301_HUMAN11 kDa1 Ig lambda chain V-IV region HilLV403_HUMAN12 kDa1 Ig lambda-2 chain C regionsLAC2_HUMAN11 kDa2 Ig mu chain C regionIGHM_HUMAN49 kDa5 Immunoglobulin J chainIGJ_HUMAN18 kDa3 Immunoglobulin lambda-like polypeptide 5IGLL5_HUMAN23 kDa5 Integrin alpha-MITAM_HUMAN127 kDa1 Interleukin-1 receptor antagonist proteinIL1RA_HUMAN20 kDa1[5] Interleukin-36 gammaIL36G_HUMAN19 kDa2 InvolucrinINVO_HUMAN68 kDa9 Kallikrein-1KLK1_HUMAN29 kDa1 Kallikrein-13KLK13_HUMAN31 kDa1 Keratin, type I cuticular Ha1K1H1_HUMAN47 kDa6 Keratin, type I cuticular Ha3-IIKT33B_HUMAN46 kDa3 Keratin, type I cytoskeletal 10K1C10_HUMAN59 kDa21 Keratin, type I cytoskeletal 13K1C13_HUMAN50 kDa21 Keratin, type I cytoskeletal 14K1C14_HUMAN52 kDa29 Keratin, type I cytoskeletal 16K1C16_HUMAN51 kDa13 Keratin, type I cytoskeletal 17K1C17_HUMAN48 kDa11 Keratin, type I cytoskeletal 19K1C19_HUMAN44 kDa6 Keratin, type I cytoskeletal 9K1C9_HUMAN62 kDa16 Keratin, type II cuticular Hb5KRT85_HUMAN56 kDa1 Keratin, type II cuticular Hb6KRT86_HUMAN53 kDa4 Keratin, type II cytoskeletal 1K2C1_HUMAN66 kDa20 Keratin, type II cytoskeletal 2 epidermalK22E_HUMAN65 kDa4 Keratin, type II cytoskeletal 2 oralK22O_HUMAN66 kDa5 Keratin, type II cytoskeletal 3K2C3_HUMAN64 kDa3 Keratin, type II cytoskeletal 4K2C4_HUMAN57 kDa18 Keratin, type II cytoskeletal 5K2C5_HUMAN62 kDa17 Keratin, type II cytoskeletal 6AK2C6A_HUMAN60 kDa38 Keratin, type II cytoskeletal 6BK2C6B_HUMAN60 kDa5 Keratin, type II cytoskeletal 6CK2C6C_HUMAN60 kDa3 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
![Identified ProteinsAccession Number Molecular Weight MS/MS(unique)References* Ig heavy chain V-III region BROHV305_HUMAN13 kDa2 Ig heavy chain V-III region GALHV320_HUMAN13 kDa1 Ig heavy chain V-III region TILHV304_HUMAN12 kDa2 Ig heavy chain V-III region TURHV318_HUMAN12 kDa1 Ig heavy chain V-III region VH26HV303_HUMAN13 kDa1 Ig kappa chain C regionIGKC_HUMAN12 kDa9 Ig kappa chain V-I region AGKV101_HUMAN12 kDa1 Ig kappa chain V-I region EUKV106_HUMAN12 kDa2 Ig kappa chain V-I region LayKV113_HUMAN12 kDa1 Ig kappa chain V-II region CumKV201_HUMAN13 kDa1 Ig kappa chain V-III region SIEKV302_HUMAN12 kDa3 Ig kappa chain V-III region VG (Fragment)KV309_HUMAN13 kDa1 Ig kappa chain V-IV region LenKV402_HUMAN13 kDa2 Ig lambda chain V-I region HALV102_HUMAN12 kDa1 Ig lambda chain V-III region LOILV302_HUMAN12 kDa1 Ig lambda chain V-III region SHLV301_HUMAN11 kDa1 Ig lambda chain V-IV region HilLV403_HUMAN12 kDa1 Ig lambda-2 chain C regionsLAC2_HUMAN11 kDa2 Ig mu chain C regionIGHM_HUMAN49 kDa5 Immunoglobulin J chainIGJ_HUMAN18 kDa3 Immunoglobulin lambda-like polypeptide 5IGLL5_HUMAN23 kDa5 Integrin alpha-MITAM_HUMAN127 kDa1 Interleukin-1 receptor antagonist proteinIL1RA_HUMAN20 kDa1[5] Interleukin-36 gammaIL36G_HUMAN19 kDa2 InvolucrinINVO_HUMAN68 kDa9 Kallikrein-1KLK1_HUMAN29 kDa1 Kallikrein-13KLK13_HUMAN31 kDa1 Keratin, type I cuticular Ha1K1H1_HUMAN47 kDa6 Keratin, type I cuticular Ha3-IIKT33B_HUMAN46 kDa3 Keratin, type I cytoskeletal 10K1C10_HUMAN59 kDa21 Keratin, type I cytoskeletal 13K1C13_HUMAN50 kDa21 Keratin, type I cytoskeletal 14K1C14_HUMAN52 kDa29 Keratin, type I cytoskeletal 16K1C16_HUMAN51 kDa13 Keratin, type I cytoskeletal 17K1C17_HUMAN48 kDa11 Keratin, type I cytoskeletal 19K1C19_HUMAN44 kDa6 Keratin, type I cytoskeletal 9K1C9_HUMAN62 kDa16 Keratin, type II cuticular Hb5KRT85_HUMAN56 kDa1 Keratin, type II cuticular Hb6KRT86_HUMAN53 kDa4 Keratin, type II cytoskeletal 1K2C1_HUMAN66 kDa20 Keratin, type II cytoskeletal 2 epidermalK22E_HUMAN65 kDa4 Keratin, type II cytoskeletal 2 oralK22O_HUMAN66 kDa5 Keratin, type II cytoskeletal 3K2C3_HUMAN64 kDa3 Keratin, type II cytoskeletal 4K2C4_HUMAN57 kDa18 Keratin, type II cytoskeletal 5K2C5_HUMAN62 kDa17 Keratin, type II cytoskeletal 6AK2C6A_HUMAN60 kDa38 Keratin, type II cytoskeletal 6BK2C6B_HUMAN60 kDa5 Keratin, type II cytoskeletal 6CK2C6C_HUMAN60 kDa3 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS.](http://images.slideplayer.com/31/9770369/slides/slide_4.jpg "Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease..")
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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Keratin, type II cytoskeletal 78K2C78_HUMAN57 kDa2 Keratinocyte proline-rich proteinKPRP_HUMAN64 kDa1 LactotransferrinTRFL_HUMAN78 kDa51 Lamin-B1LMNB1_HUMAN66 kDa2 Leukocyte elastase inhibitorILEU_HUMAN43 kDa10 L-lactate dehydrogenase A chainLDHA_HUMAN37 kDa4 L-lactate dehydrogenase B chainLDHB_HUMAN37 kDa2 Low affinity immunoglobulin gamma Fc region receptor III-AFCG3A_HUMAN29 kDa1 Ly6/PLAUR domain-containing protein 3LYPD3_HUMAN36 kDa2 Lymphocyte-specific protein 1LSP1_HUMAN37 kDa1 Lysosome-associated membrane glycoprotein 2LAMP2_HUMAN45 kDa1 Lysozyme CLYSC_HUMAN17 kDa6[6] Macrophage migration inhibitory factorMIF_HUMAN12 kDa1[7] Macrophage-capping proteinCAPG_HUMAN38 kDa2 Malate dehydrogenase, mitochondrialMDHM_HUMAN36 kDa2 Matrix metalloproteinase-9MMP9_HUMAN78 kDa6[8] Metalloproteinase inhibitor 1TIMP1_HUMAN23 kDa1[9] Mitochondrial peptide methionine sulfoxide reductaseMSRA_HUMAN26 kDa1 MoesinMOES_HUMAN68 kDa3 Mucin-5BMUC5B_HUMAN596 kDa23 Myeloid cell nuclear differentiation antigenMNDA_HUMAN46 kDa1 MyeloperoxidasePERM_HUMAN84 kDa28 Myosin light polypeptide 6MYL6_HUMAN17 kDa6 Myosin regulatory light chain 12AML12A_HUMAN20 kDa4 Myosin-9MYH9_HUMAN227 kDa28 Myosin-IeMYO1E_HUMAN127 kDa1 Na(+)/H(+) exchange regulatory cofactor NHE-RF1NHRF1_HUMAN39 kDa1 NebulinNEBU_HUMAN773 kDa1 Neuroblast differentiation-associated protein AHNAKAHNK_HUMAN629 kDa2 Neutrophil collagenaseMMP8_HUMAN53 kDa4 Neutrophil cytosol factor 2NCF2_HUMAN60 kDa1 Neutrophil defensin 1DEF1_HUMAN10 kDa3[10] Neutrophil elastaseELNE_HUMAN29 kDa5 Neutrophil gelatinase-associated lipocalinNGAL_HUMAN23 kDa11 Nicotinamide phosphoribosyltransferaseNAMPT_HUMAN56 kDa1 Non-secretory ribonucleaseRNAS2_HUMAN18 kDa3 NucleophosminNPM_HUMAN33 kDa1 Olfactomedin-4OLFM4_HUMAN57 kDa3 Peptidoglycan recognition protein 1PGRP1_HUMAN22 kDa3 Peptidyl-prolyl cis-trans isomerase APPIA_HUMAN18 kDa7 Peptidyl-prolyl cis-trans isomerase BPPIB_HUMAN24 kDa1 Peptidyl-prolyl cis-trans isomerase FKBP1AFKB1A_HUMAN12 kDa1 PeriplakinPEPL_HUMAN205 kDa1 Peroxiredoxin-1PRDX1_HUMAN22 kDa2 Peroxiredoxin-5, mitochondrialPRDX5_HUMAN22 kDa1 Phosphatidylethanolamine-binding protein 1PEBP1_HUMAN21 kDa2 Phosphoglycerate kinase 1PGK1_HUMAN45 kDa2 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
![Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Keratin, type II cytoskeletal 78K2C78_HUMAN57 kDa2 Keratinocyte proline-rich proteinKPRP_HUMAN64 kDa1 LactotransferrinTRFL_HUMAN78 kDa51 Lamin-B1LMNB1_HUMAN66 kDa2 Leukocyte elastase inhibitorILEU_HUMAN43 kDa10 L-lactate dehydrogenase A chainLDHA_HUMAN37 kDa4 L-lactate dehydrogenase B chainLDHB_HUMAN37 kDa2 Low affinity immunoglobulin gamma Fc region receptor III-AFCG3A_HUMAN29 kDa1 Ly6/PLAUR domain-containing protein 3LYPD3_HUMAN36 kDa2 Lymphocyte-specific protein 1LSP1_HUMAN37 kDa1 Lysosome-associated membrane glycoprotein 2LAMP2_HUMAN45 kDa1 Lysozyme CLYSC_HUMAN17 kDa6[6] Macrophage migration inhibitory factorMIF_HUMAN12 kDa1[7] Macrophage-capping proteinCAPG_HUMAN38 kDa2 Malate dehydrogenase, mitochondrialMDHM_HUMAN36 kDa2 Matrix metalloproteinase-9MMP9_HUMAN78 kDa6[8] Metalloproteinase inhibitor 1TIMP1_HUMAN23 kDa1[9] Mitochondrial peptide methionine sulfoxide reductaseMSRA_HUMAN26 kDa1 MoesinMOES_HUMAN68 kDa3 Mucin-5BMUC5B_HUMAN596 kDa23 Myeloid cell nuclear differentiation antigenMNDA_HUMAN46 kDa1 MyeloperoxidasePERM_HUMAN84 kDa28 Myosin light polypeptide 6MYL6_HUMAN17 kDa6 Myosin regulatory light chain 12AML12A_HUMAN20 kDa4 Myosin-9MYH9_HUMAN227 kDa28 Myosin-IeMYO1E_HUMAN127 kDa1 Na(+)/H(+) exchange regulatory cofactor NHE-RF1NHRF1_HUMAN39 kDa1 NebulinNEBU_HUMAN773 kDa1 Neuroblast differentiation-associated protein AHNAKAHNK_HUMAN629 kDa2 Neutrophil collagenaseMMP8_HUMAN53 kDa4 Neutrophil cytosol factor 2NCF2_HUMAN60 kDa1 Neutrophil defensin 1DEF1_HUMAN10 kDa3[10] Neutrophil elastaseELNE_HUMAN29 kDa5 Neutrophil gelatinase-associated lipocalinNGAL_HUMAN23 kDa11 Nicotinamide phosphoribosyltransferaseNAMPT_HUMAN56 kDa1 Non-secretory ribonucleaseRNAS2_HUMAN18 kDa3 NucleophosminNPM_HUMAN33 kDa1 Olfactomedin-4OLFM4_HUMAN57 kDa3 Peptidoglycan recognition protein 1PGRP1_HUMAN22 kDa3 Peptidyl-prolyl cis-trans isomerase APPIA_HUMAN18 kDa7 Peptidyl-prolyl cis-trans isomerase BPPIB_HUMAN24 kDa1 Peptidyl-prolyl cis-trans isomerase FKBP1AFKB1A_HUMAN12 kDa1 PeriplakinPEPL_HUMAN205 kDa1 Peroxiredoxin-1PRDX1_HUMAN22 kDa2 Peroxiredoxin-5, mitochondrialPRDX5_HUMAN22 kDa1 Phosphatidylethanolamine-binding protein 1PEBP1_HUMAN21 kDa2 Phosphoglycerate kinase 1PGK1_HUMAN45 kDa2 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS.](http://images.slideplayer.com/31/9770369/slides/slide_5.jpg "Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease..")
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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Phosphoglycerate mutase 1PGAM1_HUMAN29 kDa3 Phospholipase B-like 1PLBL1_HUMAN63 kDa1 Plasma protease C1 inhibitorIC1_HUMAN55 kDa2 PlasminogenPLMN_HUMAN91 kDa5 Plastin-2PLSL_HUMAN70 kDa9 Polymeric immunoglobulin receptorPIGR_HUMAN83 kDa9 Prelamin-A/CLMNA_HUMAN74 kDa1 Proactivator polypeptideSAP_HUMAN58 kDa2 Profilin-1PROF1_HUMAN15 kDa6 Prolactin-inducible proteinPIP_HUMAN17 kDa9 Proteasome activator complex subunit 1PSME1_HUMAN29 kDa1 Protein AMBPAMBP_HUMAN39 kDa2 Protein disulfide-isomerasePDIA1_HUMAN57 kDa4 Protein disulfide-isomerase A3PDIA3_HUMAN57 kDa1 Protein DJ-1PARK7_HUMAN20 kDa1 Protein FAM49BFA49B_HUMAN37 kDa1 Protein S100-A11S10AB_HUMAN12 kDa3 Protein S100-A12S10AC_HUMAN11 kDa1 Protein S100-A14S10AE_HUMAN12 kDa1 Protein S100-A2S10A2_HUMAN11 kDa1 Protein S100-A6S10A6_HUMAN10 kDa1 Protein S100-A7S10A7_HUMAN11 kDa5 Protein S100-A8S10A8_HUMAN11 kDa12 Protein S100-A9S10A9_HUMAN13 kDa12 Protein S100-PS100P_HUMAN10 kDa2 Protein-glutamine gamma-glutamyltransferase ETGM3_HUMAN77 kDa11 ProthrombinTHRB_HUMAN70 kDa1 Purine nucleoside phosphorylasePNPH_HUMAN32 kDa2 Putative tropomyosin alpha-3 chain-like proteinTPM3L_HUMAN26 kDa1 Pyridoxal kinasePDXK_HUMAN35 kDa1 Pyruvate kinase isozymes M1/M2KPYM_HUMAN58 kDa12 Rab GDP dissociation inhibitor betaGDIB_HUMAN51 kDa1 Ras GTPase-activating-like protein IQGAP1IQGA1_HUMAN189 kDa4 Ras-related C3 botulinum toxin substrate 1RAC1_HUMAN21 kDa1 Ras-related protein Rab-10RAB10_HUMAN23 kDa1 Ras-related protein Rab-11ARB11A_HUMAN24 kDa1 Ras-related protein Rab-1ARAB1A_HUMAN23 kDa3 ResistinRETN_HUMAN11 kDa2 Retinol-binding protein 4RET4_HUMAN23 kDa1 Rho GDP-dissociation inhibitor 1GDIR1_HUMAN23 kDa2 Rho GDP-dissociation inhibitor 2GDIR2_HUMAN23 kDa6 Serine/threonine-protein kinase PAK 2PAK2_HUMAN58 kDa1 SerotransferrinTRFE_HUMAN77 kDa49 Serpin B3SPB3_HUMAN45 kDa7 Serum albuminALBU_HUMAN69 kDa64 SH3 domain-binding glutamic acid-rich-like proteinSH3L1_HUMAN13 kDa3 SH3 domain-binding glutamic acid-rich-like protein 3SH3L3_HUMAN10 kDa1 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
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Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Small proline-rich protein 2ASPR2A_HUMAN8 kDa1 Small proline-rich protein 2DSPR2D_HUMAN8 kDa4 Small proline-rich protein 2ESPR2E_HUMAN8 kDa1 Small proline-rich protein 2FSPR2F_HUMAN8 kDa1 Small proline-rich protein 3SPRR3_HUMAN18 kDa5 Submaxillary gland androgen-regulated protein 3BSMR3B_HUMAN8 kDa3 Superoxide dismutase [Cu-Zn]SODC_HUMAN16 kDa2[11] Superoxide dismutase [Mn], mitochondrialSODM_HUMAN25 kDa5[11] SuprabasinSBSN_HUMAN25 kDa1 Synaptic vesicle membrane protein VAT-1 homologVAT1_HUMAN42 kDa1 Talin-1TLN1_HUMAN270 kDa1 ThioredoxinTHIO_HUMAN12 kDa6 Thymosin beta-4-like protein 3TMSL3_HUMAN5 kDa3 TransaldolaseTALDO_HUMAN38 kDa1 Transcobalamin-1TCO1_HUMAN48 kDa1 Transgelin-2TAGL2_HUMAN22 kDa2 Transitional endoplasmic reticulum ATPaseTERA_HUMAN89 kDa1 TransketolaseTKT_HUMAN68 kDa15 TransthyretinTTHY_HUMAN16 kDa4 Trefoil factor 3TFF3_HUMAN9 kDa1 Triosephosphate isomeraseTPIS_HUMAN27 kDa13 Tripartite motif-containing protein 29TRI29_HUMAN66 kDa1 Tropomyosin alpha-3 chainTPM3_HUMAN33 kDa3 Trypsin-3TRY3_HUMAN33 kDa1 Tubulin beta-2A chainTBB2A_HUMAN50 kDa1 Ubiquitin-60S ribosomal protein L40RL40_HUMAN15 kDa2 Ubiquitin-like modifier-activating enzyme 1UBA1_HUMAN118 kDa1 UMP-CMP kinaseKCY_HUMAN22 kDa1 UPF0762 protein C6orf58CF058_HUMAN38 kDa1 Vasodilator-stimulated phosphoproteinVASP_HUMAN40 kDa1 Versican core proteinCSPG2_HUMAN373 kDa1 VimentinVIME_HUMAN54 kDa15 Vitamin D-binding proteinVTDB_HUMAN53 kDa2[12] VitronectinVTNC_HUMAN54 kDa2 WD repeat-containing protein 1WDR1_HUMAN66 kDa1 Zinc-alpha-2-glycoproteinZA2G_HUMAN34 kDa9 Zymogen granule protein 16 homolog BZG16B_HUMAN23 kDa2 14-3-3 protein gamma1433G_HUMAN28 kDa1 14-3-3 protein sigma1433S_HUMAN28 kDa10 14-3-3 protein zeta/delta1433Z_HUMAN28 kDa4 40S ribosomal protein S18RS18_HUMAN18 kDa1 60S acidic ribosomal protein P2RLA2_HUMAN12 kDa1 60S ribosomal protein L7aRL7A_HUMAN30 kDa1 6-phosphogluconate dehydrogenase, decarboxylating6PGD_HUMAN53 kDa3 78 kDa glucose-regulated proteinGRP78_HUMAN72 kDa6 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS. Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease.
![Identified ProteinsAccession Number a) Molecular Weight b) MS/MS(unique) c) References* Small proline-rich protein 2ASPR2A_HUMAN8 kDa1 Small proline-rich protein 2DSPR2D_HUMAN8 kDa4 Small proline-rich protein 2ESPR2E_HUMAN8 kDa1 Small proline-rich protein 2FSPR2F_HUMAN8 kDa1 Small proline-rich protein 3SPRR3_HUMAN18 kDa5 Submaxillary gland androgen-regulated protein 3BSMR3B_HUMAN8 kDa3 Superoxide dismutase [Cu-Zn]SODC_HUMAN16 kDa2[11] Superoxide dismutase [Mn], mitochondrialSODM_HUMAN25 kDa5[11] SuprabasinSBSN_HUMAN25 kDa1 Synaptic vesicle membrane protein VAT-1 homologVAT1_HUMAN42 kDa1 Talin-1TLN1_HUMAN270 kDa1 ThioredoxinTHIO_HUMAN12 kDa6 Thymosin beta-4-like protein 3TMSL3_HUMAN5 kDa3 TransaldolaseTALDO_HUMAN38 kDa1 Transcobalamin-1TCO1_HUMAN48 kDa1 Transgelin-2TAGL2_HUMAN22 kDa2 Transitional endoplasmic reticulum ATPaseTERA_HUMAN89 kDa1 TransketolaseTKT_HUMAN68 kDa15 TransthyretinTTHY_HUMAN16 kDa4 Trefoil factor 3TFF3_HUMAN9 kDa1 Triosephosphate isomeraseTPIS_HUMAN27 kDa13 Tripartite motif-containing protein 29TRI29_HUMAN66 kDa1 Tropomyosin alpha-3 chainTPM3_HUMAN33 kDa3 Trypsin-3TRY3_HUMAN33 kDa1 Tubulin beta-2A chainTBB2A_HUMAN50 kDa1 Ubiquitin-60S ribosomal protein L40RL40_HUMAN15 kDa2 Ubiquitin-like modifier-activating enzyme 1UBA1_HUMAN118 kDa1 UMP-CMP kinaseKCY_HUMAN22 kDa1 UPF0762 protein C6orf58CF058_HUMAN38 kDa1 Vasodilator-stimulated phosphoproteinVASP_HUMAN40 kDa1 Versican core proteinCSPG2_HUMAN373 kDa1 VimentinVIME_HUMAN54 kDa15 Vitamin D-binding proteinVTDB_HUMAN53 kDa2[12] VitronectinVTNC_HUMAN54 kDa2 WD repeat-containing protein 1WDR1_HUMAN66 kDa1 Zinc-alpha-2-glycoproteinZA2G_HUMAN34 kDa9 Zymogen granule protein 16 homolog BZG16B_HUMAN23 kDa protein gamma1433G_HUMAN28 kDa protein sigma1433S_HUMAN28 kDa protein zeta/delta1433Z_HUMAN28 kDa4 40S ribosomal protein S18RS18_HUMAN18 kDa1 60S acidic ribosomal protein P2RLA2_HUMAN12 kDa1 60S ribosomal protein L7aRL7A_HUMAN30 kDa1 6-phosphogluconate dehydrogenase, decarboxylating6PGD_HUMAN53 kDa3 78 kDa glucose-regulated proteinGRP78_HUMAN72 kDa6 GCF proteins from healthy subjects (n=5) were identified using LC-MS/MS.](http://images.slideplayer.com/31/9770369/slides/slide_7.jpg "Identified proteins are listed by alphabetical order. a) Reference for protein identification b) Theoretical molecular mass based on Swiss-Prot database c) MS/MS unique peptide * Previously reported proteins involved in periodontal disease..")
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References [1] Ebersole, J. L., Cappelli, D., Mott, G., Kesavalu, L., et al., Systemic manifestations of periodontitis in the non-human primate. J. Periodontal. Res. 1999, 34, 358-62.Systemic manifestations of periodontitis in the non-human primate. [2] Weinberg, A., Krisanaprakornkit, S., Epithelial antimicrobial peptides: review and significance for oral applications. Dale. BACrit. Rev. Oral. Biol. Med. 1998, 9, 399-414.Weinberg, AKrisanaprakornkit, SDale. BACrit. Rev. Oral. Biol. Med. [3] Younes, R., Yousfi, M., Ghorra, C., Khalife, S., The defensive role of lysozyme in human gingiva in inflammatory periodontal disease. J. Periodontal. Res. 2009, 44, 578-87.Younes, RYousfi, MGhorra, CKhalife, SJ. Periodontal. Res. [4] Tsai, C. H., Yang, S. F., Huang, F. M., Chang, Y. C., The upregulation of cystatin C in human gingival fibroblasts stimulated with cyclosporine A. J. Periodontal. Res. 2009, 44, 459-64.Tsai, C. HYang, S. FHuang, F. MChang, Y. CJ. Periodontal. Res. [5] Toker, H., Poyraz, O., Eren, K., Effect of periodontal treatment on IL- 1beta, IL-1ra, and IL-10 levels in gingival crevicular fluid in patients with aggressive periodontitis. J. Clin. Periodontol. 2008, 35, 507-13.Toker, HPoyraz, OEren, KJ. Clin. Periodontol. [6] Surna, A., Kubilius, R., Sakalauskiene, J., Vitkauskiene, A., et al., Lysozyme and microbiota in relation to gingivitis and periodontitis. Med. Sci. Monit. 2009, 15, 66-73.Surna, AKubilius, RSakalauskiene, JVitkauskiene, AMed. Sci. Monit. [7] Nonnenmacher, C., Helms, K., Bacher, M., Nüsing, R., Effect of age on gingival crevicular fluid concentrations of MIF and PGE2. J. Dent. Res. 2009, 88, 639-43.Nonnenmacher, CHelms, KBacher, MNüsing, R., J. Dent. Res. [8] Verstappen, J., Von den Hoff, J.W., Tissue inhibitors of metalloproteinases (TIMPs): their biological functions and involvement in oral disease. J. Dent. Res. 2006, 85, 1074-84.Verstappen, JVon den Hoff, J.WJ. Dent. Res. [9] Laaksonen, M., Salo, T., Vardar-Sengul, S., Atilla, G., et al., Gingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of periodontal ligament fibroblasts. J. Periodontal. Res. 2010, 45, 353-60.Laaksonen, MSalo, TVardar-Sengul, SAtilla, GJ. Periodontal. Res. [10] Lee, S. H., Jun, H. K., Lee, H. R., Chung, C.P., et al. Antibacterial and lipopolysaccharide (LPS)-neutralising activity of human cationic antimicrobial peptides against periodontopathogens. Int. J. Antimicrob. Agents. 2010, 35, 138-45.Lee, S. HJun, H. KLee, H. RChung, C.PInt. J. Antimicrob. Agents. [11] Agnihotri, R., Pandurang, P., Kamath, S.U., Goyal, R., Association of cigarette smoking with superoxide dismutase enzyme levels in subjects with chronic periodontitis. J. Periodontol. 2009, 80, 657-62.Agnihotri, RPandurang, PKamath, S.UGoyal, RJ. Periodontol. [12] Hart,T. C., Marazita, M. L., McCanna, K, M., Schenkein, H. A., et al., Reevaluation of the chromosome 4q candidate region for early onset periodontitis. Hum. Genet. 1993, 91, 416-22.Hart,T. CMarazita, M. LMcCanna, K, MSchenkein, H. AHum. Genet.
![References [1] Ebersole, J.](http://images.slideplayer.com/31/9770369/slides/slide_8.jpg "L., Cappelli, D., Mott, G., Kesavalu, L., et al., Systemic manifestations of periodontitis in the non-human primate. J. Periodontal. Res. 1999, 34, Systemic manifestations of periodontitis in the non-human primate. [2] Weinberg, A., Krisanaprakornkit, S., Epithelial antimicrobial peptides: review and significance for oral applications. Dale. BACrit. Rev. Oral. Biol. Med. 1998, 9, Weinberg, AKrisanaprakornkit, SDale. BACrit. Rev. Oral. Biol. Med. [3] Younes, R., Yousfi, M., Ghorra, C., Khalife, S., The defensive role of lysozyme in human gingiva in inflammatory periodontal disease. J. Periodontal. Res. 2009, 44, Younes, RYousfi, MGhorra, CKhalife, SJ. Periodontal. Res. [4] Tsai, C. H., Yang, S. F., Huang, F. M., Chang, Y. C., The upregulation of cystatin C in human gingival fibroblasts stimulated with cyclosporine A. J. Periodontal. Res. 2009, 44, Tsai, C. HYang, S. FHuang, F. MChang, Y. CJ. Periodontal. Res. [5] Toker, H., Poyraz, O., Eren, K., Effect of periodontal treatment on IL- 1beta, IL-1ra, and IL-10 levels in gingival crevicular fluid in patients with aggressive periodontitis. J. Clin. Periodontol. 2008, 35, Toker, HPoyraz, OEren, KJ. Clin. Periodontol. [6] Surna, A., Kubilius, R., Sakalauskiene, J., Vitkauskiene, A., et al., Lysozyme and microbiota in relation to gingivitis and periodontitis. Med. Sci. Monit. 2009, 15, Surna, AKubilius, RSakalauskiene, JVitkauskiene, AMed. Sci. Monit. [7] Nonnenmacher, C., Helms, K., Bacher, M., Nüsing, R., Effect of age on gingival crevicular fluid concentrations of MIF and PGE2. J. Dent. Res. 2009, 88, Nonnenmacher, CHelms, KBacher, MNüsing, R., J. Dent. Res. [8] Verstappen, J., Von den Hoff, J.W., Tissue inhibitors of metalloproteinases (TIMPs): their biological functions and involvement in oral disease. J. Dent. Res. 2006, 85, Verstappen, JVon den Hoff, J.WJ. Dent. Res. [9] Laaksonen, M., Salo, T., Vardar-Sengul, S., Atilla, G., et al., Gingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of periodontal ligament fibroblasts. J. Periodontal. Res. 2010, 45, Laaksonen, MSalo, TVardar-Sengul, SAtilla, GJ. Periodontal. Res. [10] Lee, S. H., Jun, H. K., Lee, H. R., Chung, C.P., et al. Antibacterial and lipopolysaccharide (LPS)-neutralising activity of human cationic antimicrobial peptides against periodontopathogens. Int. J. Antimicrob. Agents. 2010, 35, Lee, S. HJun, H. KLee, H. RChung, C.PInt. J. Antimicrob. Agents. [11] Agnihotri, R., Pandurang, P., Kamath, S.U., Goyal, R., Association of cigarette smoking with superoxide dismutase enzyme levels in subjects with chronic periodontitis. J. Periodontol. 2009, 80, Agnihotri, RPandurang, PKamath, S.UGoyal, RJ. Periodontol. [12] Hart,T. C., Marazita, M. L., McCanna, K, M., Schenkein, H. A., et al., Reevaluation of the chromosome 4q candidate region for early onset periodontitis. Hum. Genet. 1993, 91, Hart,T. CMarazita, M. LMcCanna, K, MSchenkein, H. AHum. Genet..")
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