1. FLAG-PAWS1 HA-SMAD1 BMP GFP-SMAD4 +-+++--- ----++++ +++--+++ +--+++-- IP: GFP IB: HA IB: FLAG IB: P-SMAD1 S206 IB: GFP IB: P-SMAD1 S206 IB: HA IB: FLAG IB: P-SMAD1 IB: GFP Input Vogt et al. Figure S1 A IB: SMAD1 Input IP: SMAD1 -TGFßBMP IgG D +---++---+ GFP GFP-SMAD1 GFP-SMAD5 GFP-SMAD8 FLAG-PAWS1 -+--+-+--+ --+-+--+-+ ---++---++ IB: PAWS1 IB: GFP IP-FLAG input HEK293 B +----++----+ FLAG FLAG-SMAD1 FLAG-SMAD1 MH1 FLAG-SMAD1 Linker FLAG-SMAD1 MH2 HA-PAWS1 -+---+-+---+ --+--+--+--+ ---+-+---+-+ IB: HA-PAWS1 IB: FLAG IP-FLAG input HEK293 IB: FLAG IB: HA-PAWS1 ----++----++ C

2. A MDA-MB-231U2OSHEK 293RKOHaCaTHeLaMCF7PC3KBM7 IB: PAWS1 low IB: PAWS1 high IB: SMAD1 IB: GAPDH B IB: PAWS1 low IB: PAWS1 high IB: SMAD1 IB: GAPDH PAWS1 BrainHeartLungKidneyLiverSpleenThymusPancreasAdipose tissueMuscleTestisEpididymisOvaries Vogt et al. Figure S2

3. 32 P-Autorad LDN-193189 (µM) 0.0000.0010.0030.0100.0300.1000.3001.0003.00010.00 ALK2 Coomassie 0.0000.0010.0030.0100.0300.1000.3001.0003.00010.00 ALK6 Vogt et al. Figure S3 Phospho-PAWS1

4. Peak1Peak2 BMPR1 + GST-PAWS1(S610A) Vogt et al. Figure S4 A B C P1 P2 662.6184 +2P 953.4414 +P

5. Vogt et al. Figure S5 IP: PAWS1; IB: PAWS1 IB: P-SMAD1 IB: SMAD1 IB: GAPDH IP: PAWS1; IB: P-PAWS1 S610 BMP for min.01545120240480

6. BxPC3 -BMP 2BMP 4/7 0 1 2 3 ASNS NEDD9 fold change Vogt et al. Figure S6 A B P<0.001

7. TGFBR2 0.0 0.5 1.0 1.5 2.0 2.5 fold change TSC22D 0.0 0.5 1.0 1.5 fold change unstimulated BMP-2 Vogt et al. Figure S7 PC3- control PC3- PAWS1 PC3- control PC3- PAWS1 ID1 0 10 20 30 40 fold change PC3- control PC3- PAWS1 P<0.0001 ID1 TGFBR2 TSC22D AB C P<0.0001 P=0.0007 P=0.0029