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Figure 2 GlyR antibody binding

Published byEsther Aguirre Rojas Modified over 5 years ago

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Presentation on theme: "Figure 2 GlyR antibody binding"— Presentation transcript:

1 Figure 2 GlyR antibody binding
GlyR antibody binding Antibodies bind to extracellular domain of glycine receptor subunits. HEK293 cells were cotransfected with GFP and with different glycine receptor subunits 1, 2, 3. Cells were incubated with purified patient IgG (I–L) and IgG from a healthy control (E–H) in a 2 mg/mL concentration. The GlyR 1–specific antibody MAb2B was used as a positive control, as well as the pan-antibody MAb4A to stain 2 and 3 (A–D). Of note, the surface staining of all GlyR subunits upon incubation with the patient's IgG is only present in live cells (A–C, E–G, I–K), not in fixed and permeabilized cells (D, H, L), while the control antibodies bind in both conditions. This shows that the antibodies in patient serum bind only to the glycine receptors in their native state. White bar = 10 μm. GFP = green fluorescent protein; GlyR = glycine receptor; IgG = immunoglobulin G; MAb = monoclonal antibody. Kathrin Doppler et al. Neurol Neuroimmunol Neuroinflamm 2016;3:e186 © 2015 American Academy of Neurology

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