1. Important Lab instrument
Microscope
Important Lab instrument

2. Why use a microscope?
To view objects and detail too small to see with human eye. Improves resolution of object
List examples of how what you used it for:
Blood cell detail
rbc, wbc, platelets
Bacteria
Cocci, rod, bacillus
Protozoa
Trichomonas
Giardia

3. Types of Microscopes Compound microscope Electron microscope
Bacteria, fungi and protozoa
Electron microscope
Required for viruses
Fluorescence
Used as a diagnostic tool for immunofluorescence tests

4. Parts of a microscope Coarse adjustment Fine adjustment Stage
1st step in focusing to change the distance between specimen and lens
Fine adjustment
To fine tune the picture
Used particularly with 100x and oil objective
Stage
Holds the slide, moved up and down with coarse and fine adjustment

5. Parts of a microscope Objectives
Common objectives are 4x, 10x, 40x, 100x, oil
Total magnification is eye piece magnification multiplied times objective you are viewing with
40x objective and 10x eye piece is 400x magnification
Oil objective is labeled and is always used with immersion oil
Oil increases the resolving power by focusing the light rays

6. Proper care for microscope
Always start with lowest objective to focus
Always store with lowest objective locked in place
Carry with two hands
Cover with dust cover
Be sure to clean oil off of oil objective
Use fine adjustment with 100x and oil objective

7. THE COMPONENTS OF THE MICROSCOPE
What do you remember?

8. THE MICROSCOPE EYEPIECE What is it when there is only 1?
When there are 2?

9. THE MICROSCOPE
ARM

10. THE MICROSCOPE
BASE

11. THE MICROSCOPE
TUBE

12. THE MICROSCOPE
REVOLVING NOSEPIECE

13. THE MICROSCOPE OBJECTIVE LENS
What are the magnification of the 4 objectives?
4, 10, 40, 100x
What magnification is the eye piece?
10x unless otherwise stated
What would be the total magnification when viewing an object through the 40x objective?
400x

14. THE MICROSCOPE MECHANICAL STAGE
Why is a mechanical stage better than using clips?

15. THE MICROSCOPE IRIS DIAPHRAGM What does this do?
Control the amount of light

16. THE MICROSCOPE COARSE ADJUSTMENT KNOB
What 2 objectives do you use the coarse adjustment?
4 and 10x

17. THE MICROSCOPE FINE ADJUSTMENT KNOB What objectives is this used with?
All, but mostly 40, 100x

18. THE MICROSCOPE
Light

19. THE MICROSCOPE
ON/OFF SWITCH

20. CARE OF THE MICROSCOPE
Always carry the microscope by the arm and the base and use 2 hands!

21. CARE OF THE MICROSCOPE
Wrap the cord up or around the microscope so it is not hanging down.

22. CARE OF THE MICROSCOPE
Always lower the stage and clean all objectives off
Always use a dust cover!