1. Running GC

2. Running GC Launch the “TotalChrom” Software Click on “Run”
Select “Take Control…”
Select “Clarus 580”
Click “OK”

3. Running GC
The GC touch screen panel should show that the GC is in external control.
Once in external control:
Build Method
Build Sequence

4. Build Method

5. Build Method
Click on “Method” button

6. Build Method
Select “Create new method”
Click on “OK”

7. Build Method Type in a description Click “Next” Type in a header text
The Data Acquisition window should look like the following
Click “Next”

8. Build Method Autosampler tab should look like the following
Adjust as you see fit
What is shown is a good standard
Click “Oven/Inlets” Tab

9. Build Method Adjust the Oven Ramp program as directed.
Make sure to hit enter on the keyboard after every entry
Adjust the “Injector A” temperature. Typically this is set around 225 C.
Click on “Carrier” Tab

10. Build Method Adjust the Carrier tab as directed.
Typical settings are shown.
Click on “Detectors” Tab

11. Build Method Adjust the Detectors tab as directed.
Typical settings are shown.
Click on “Next”

12. Build Method (No Need to Mess with) Click “Next” Click “Next”
Click “Finish”
Click “Next”

13. Build Method File  Save Create a unique name Click on “Save”
Close Method window

14. Build Sequence

15. Build Sequence
Click on “Sequence” button

16. Build Sequence
Select “Create new sequence”
Click on “OK”

17. Build Sequence
Select the listed options
Click on “OK”

18. Build Sequence Type in a unique name Type in which vial to be used
Select a method (brings up a new window)
Click on “Add+Close” if all vials are added or click on “Add” to add more vials

19. Build Sequence File  Save Create a unique name Click on “Save”
Close Sequence window

20. Running Sequence

21. Running Sequence
Click on “Setup” button

22. Running Sequence Select a sequence (new window will open / not shown)
Click OK

23. Running Sequence
The GC will take some time to upload the sequence information, equilibrate, and do pre-run operation
You will know that the sequence upload properly because the Instrument-Hands On and Instrument-Modify will no longer be grayed out
Once the everything is ready the lettering here should be in green.
I/F will read Ready
Click on Run
Start Run