2. Conclusion My hypothesis was correct, as the concentration levels grew the Fat Head minnows began to dwindle. The amount of chlorine that caused the most death was 50% concentration. Even at some of the lower concentration the fish began to show signs of illness. In years to come their may be a solution to solving the use of chlorine and prohibiting its use. Through this experiment maybe society will realize we are all part of this world even the smallest of creatures to the largest. Abstract *There are many substances that find their way into rivers, lakes, and ponds. I decided to test the effects of chlorine on Fat Head minnows (Pimephales promela). *By taking 30 Fat Head minnows and placing them in a control environment and then introducing different amounts of chlorine to each group. * I am hoping to find a connection between chlorine and the death of many Fat Head minnows. If my hypothesis is correct I will then determine the concentration of chlorine that was lethal. Introduction *In various ecological communities, such as ponds, lakes, and rivers chlorine, the main filter for water in human society, has always been traced or found in these different places where various species of fish live (Chubb, 1970). *In various ecological communities, such as ponds, lakes, and rivers chlorine, the main filter for water in human society, has always been traced or found in these different places where various species of fish live (Chubb, 1970). *The water from various places will be the control and various amounts of chlorine will be added to each specimen. The end results will establish if chlorine is harmful on the fish community and if so what effect chlorination could have on human beings. *The water from various places will be the control and various amounts of chlorine will be added to each specimen. The end results will establish if chlorine is harmful on the fish community and if so what effect chlorination could have on human beings. Methods * Area of interested is to monitor the lifespan of any given specimen (minnow) and determine at what chlorine concentration it was at. * Area of interested is to monitor the lifespan of any given specimen (minnow) and determine at what chlorine concentration it was at. * To determine the biological effect of chlorine when introduced into a fish environment. * To determine the biological effect of chlorine when introduced into a fish environment. * The level of concentration of chlorine which caused death. * The level of concentration of chlorine which caused death. Results Graph 1- Graph 1- The graph represents the percent total of death and living in each concentration. Graph 1- Figure 1- The figure represents that everything in the ecological community has an effect on each other. Figure 1 Materials Pimephales promelas) *30 Fish (Pimephales promelas) *6 holding containers *5 gallons of Chlorine * Fish food *aerator Timothy Lee Elliott Clarkrange, TN Tennessee Technological University Power Point for Biological Communication April 25, 2007 The Biological Effects of Chlorine on Fat Head Minnows (Pimephales promelas) Timothy Lee Elliott Clarkrange, TN Tennessee Technological University Power Point for Biological Communication April 25, 2007 Graph 1 The percentage death rateTable 1 The percentage of death per day(s) Control LivingDeath Chlorine concentration