1. NERC Environmental Bioinformatics Centre Loading annotated spreadsheets into maxdLoad2

2. NERC Environmental Bioinformatics Centre The maxdLoad2 database Browse Edit Load

3. NERC Environmental Bioinformatics Centre Worksheets with experiment data in maxdLoad2 format

4. NERC Environmental Bioinformatics Centre Spreadsheet overview

5. NERC Environmental Bioinformatics Centre Comment boxes are available on each worksheet to display help text maxdLoad2 attribute name Field Data Requirement describes the format of the data and if it is a compulsory field within maxdLoad2 Name of table within maxdLoad2 The number with dollar prefix is used to match the data to the table fields within maxdLoad2 Worksheet layout

6. NERC Environmental Bioinformatics Centre Using “presets” to load spreadsheets into maxdLoad2 Data can be entered into maxdLoad2 by one of two methods. Entering data directly into the database using the “Create” option. Loading data from a text file or Excel spreadsheet using the “Load” option.

7. NERC Environmental Bioinformatics Centre The Load option allows data from worksheets and text files to be entered in a batch format. The preset file adds a dollar prefixed number into each attribute field of the maxdLoad2 table that corresponds to a column of data in the worksheet.

8. NERC Environmental Bioinformatics Centre A single worksheet can be selected from a spreadsheet and loaded into the database. The data from the worksheet can be previewed prior to upload to ensure the data is correct.

9. NERC Environmental Bioinformatics Centre After loading the worksheet, the data can be viewed using the Browse function. In this example, only the Name and Title have been loaded from the worksheet. Browsing the loaded data

10. NERC Environmental Bioinformatics Centre We recommend that the data tables are loaded in the following order:- Sample Treated Sample Extract Labelled Extract Array Order of loading Source Sampling Protocol Treatment Protocol Extraction Protocol Labelling Protocol Hybridisation Protocol Scanning Protocol Image Analysis Protocol Hybridisation Image Measurement Submitter Experiment

11. NERC Environmental Bioinformatics Centre Fast to annotate experiments Annotate to MIAME or MIAME/Env standards Simple auto-naming generation of samples (making the experiment and relationships of samples easy to follow) Incorporates data fields for lab-specific id's Easy to up-load into maxdLoad2 Easy to customize Benefits of using the spreadsheet

12. NERC Environmental Bioinformatics Centre NEBC Oxford Dawn Field Bela Tiwari Tim Booth Stewart Houten Ashley Morley NEBC Manchester Andy Brass Dave Hancock Norman Morrison University of Cardiff Pete Kille University of Manchester Kevin Garwood Giles Velarde