1. Altacor™ WG Insecticide (DPX-E2Y45) Introducing a Novel Anthranilic Diamide Insecticide from DuPont Daniel W. Sherrod DuPont Crop Protection Memphis Market Center Memphis, TN 38017

2. DuPont Crop Protection  Code: DPX-E2Y45  Common Name: Chlorantraniliprole (ISO) - Rynaxypyr TM  CAS:3-Bromo-N-[4-chloro-2-methyl-6- [(methylamino)carbonyl]phenyl]-1- (3-chloro-2-pyridinyl)-1H-pyrazole-5- carboxamide  Chemical Structure: N H N H O O N N N Br Cl CH 3 CH 3  Empirical Formula: C 18 H 14 BrCl 2 N 5 O 2  Molecular Weight: 483.15 g/mole

3. DuPont Crop Protection Activates Insect Ryanodine Receptors Rynaxypyr™ EC 50 = 40 nM  Rynaxypyr™ and related anthranilic diamides stimulate release of Ca 2+ stores  Rynaxypyr™ is selective for ryanodine receptors  Insecticidal activity is highly correlated with Ca 2+ store release

4. DuPont Crop Protection Does Not Impact Commercialized Targets Targets Below Reflect MoA for > 90% of Commercial Insecticides ACh Excitatory Neuron Motor Neuron Muscle GABA Glut AChE Na + /K + channels M M OPs Carbamates Mitochondrial Insecticides Neonicotinoids Spinosad Pyrethroids Indoxacarb Cyclodienes Fipronil Insect Neuromuscular Signaling Pathway

5. DuPont Crop Protection What is the Ryanodine Receptor (RyR)?  Calcium channel  Regulates release of stored calcium  Critical for muscle contraction  Complex includes accessory proteins  Previously, no synthetic RyR agents known with insecticidal properties Ca 2+ FKBP Triadin Junctin lumen cytosol CAM Ryanodine Receptor Complex CSQ RyR Ryanodine (Natural Plant Toxin)

6. DuPont Crop Protection Rapidly Affects Skeletal and Cardiac Muscle  Rapid feeding cessation  General lethargy  Partial paralysis  Regurgitation Insect Poisoning Symptoms

7. DuPont Crop Protection Resistance Management Baseline Susceptibility  Heliothines  Australia, Brazil, India, Spain, USA  Spodoptera spp.  Brazil, Indonesia, Japan, Malaysia, Spain, Thailand, USA, Vietnam

8. DuPont Crop Protection Effects of DPX-E2Y45 in the Environment Soil Surface water Birds and Mammals Negligible risk Bees Negligible risk in field studies Earthworms: Negligible risk Soil Micro: Negligible risk Fish: Negligible risk Algae: Negligible risk Aquatic Inverts:  High acute toxicity  Moderate chronic toxicity Sediment Chironomus: Negligible to moderate risk Non-target Insects T1: Negligible toxicity to standard species (mites, parasitoids) + Chrysoperla, Orius T2 to T4: Safety to predatory larvae of Coccinellids and Episyrphus Plants Negligible risk No/low transport to flowering parts

9. DuPont Crop Protection Mammalian Toxicity  E2Y45 exhibits a remarkably favorable toxicity profile  Little or no toxicological effects have been observed following single doses as high as 5000 mg/kg bw and following repeat doses as high as ~1500 mg/kg bw/day  The low toxicity of this product combined with low use rates provide large margins of safety for consumers and workers

10. DuPont Crop Protection E2Y45: Mammalian Toxicology  Lack of adverse effects for the following endpoints:  Acute oral, dermal and inhalation toxicity  Skin and eye irritation; skin sensitization  Genotoxicity in vitro and in vivo  Subacute and subchronic toxicity in rats, mice, and dogs  Reproductive and developmental toxicity  Neurotoxicity  Immunotoxicity  Chronic toxicity after 1 year in dogs  Chronic toxicity after 1 and 2 years in rats  Oncogenicity after 2 years in rats and 18 months in mice

11. DuPont Crop Protection 350 to >3000X Selectivity Rynaxypyr™ Exhibits Insect Receptor Selectivity Insect Receptor: Single isoform Mammalian Receptor: RyR1 – “skeletal” RyR2 – “cardiac” RyR3 – “non-specific” Cell Line Source Expressed Isoforms PC12RatRyR2 & RyR1 C2C12MouseRyR1 & RyR3 IMR32HumanNot Defined Normalized Response (% of max) Rynaxypyr TM Concentration (log M) Insect Periplaneta Drosophila Heliothis Mammalian C 2 C 12 EC 50 = 14  m PC12EC 50 > 100  m IMR32 EC 50 > 100  m

12. DuPont Crop Protection ORDERFAMILYSPECIESRATE (g ai/Ha) NeuropteraChrysopidae Chrysoperla carnea25-100 Mallada signatus30-60 ColeopteraCoccinellidae Hippodamia sp.25 Hippodamia variegatta30 Harmonia axyridis50-100 HemipteraNabidae Nabis kimbergii30 AnthrocoridaeOrius insidiosus50 Anthocoris nemoralis60 Lygaeidae Geocoris punctipes50 AcariPhytoseiidae Amblyseius herbicolus100 Euseius citrifolius100 Iphiseiodes zulugai100 Typhlodromus occidentalis50-100 HymenopteraTrichogrammatidae Trichogramma pretiosum60 Trichogramma chilonis33 Braconidae Bracon hebetor50 Dolichogidea tasmanica30-60 Encyrtidae Ageniaspis citricola50 Aphelinidae Aphelinus mali30-60 █ no impact, (0-30% mortality); (Ratings according to IOBC/ WPRS Working Group, Hassan et al. 1988). Selectivity to Beneficial Arthropods

13. DuPont Crop Protection  Noctuidae  Heliothines (bollworms, budworms), Earias spp. (bollworms)  Spodoptera spp. (armyworms), Agrotis (cutworms)  Pseudoplusia, Trichoplusia (loopers)  Alabama argillaceae (leaf worms)  Tortricidae  Argytotaenia, Choristoneura (leafrollers)  Carposina,Cydia,Grapholita (fruit moths), Lobesia (berry moths)  Crambidae  Chilo (stem borers), Ostrinia (corn borers)  Hellula (cabbage worms)  Lerodea (leaf folders)  Leucinodes, Neoleucinodes (fruit borers)  Gelechiidae, Pieridae, Plutellidae  Anarsia (twig borers), Tuta, Keiferia (pin worms)  Pieris (whites, sulfur)  Plutella xylostella (diamondback moth)  Gracillariidae  Phyllonorycter, Phyllocnistis (leafminers) Pest Spectrum – Lepidoptera

14. DuPont Crop Protection Formulations  20 SC  200 g/L suspension concentrate  1.667 lb ai/gal suspension concentrate  Coragen TM  35 WG  35% water dispersible granule  Altacor TM  Formulation Studies  Excellent compatibility with other insecticides/fungicides  Excellent spray tank clean-out properties  Excellent mixing and handling characteristics

15. DuPont Crop Protection Product Attributes  Excellent plant protection on a broad spectrum of Lepidoptera and other key pest species, effective at low rates of application  Long-lasting protection and rainfast  Excellent fit in IPM & IRM programs  Controls insecticide-resistant strains  Low toxicity to wildlife such as birds, fish, and mammals

16. DuPont Crop Protection DuPont Rynaxypyr ™ Biological Attributes of A Novel Anthranilic Diamide Insecticide in a New Class of Potent and Selective Ryanodine Receptor Agonists

17. DuPont Crop Protection  Rynaxypyr TM acts very fast! In this experiment, Rynaxypyr TM was signifincalty faster to stop S. exigua larval feeding than Emamectin Benzoate and Methoxyfenozide DuPont Rynaxypyr TM Rapid Feeding Cessation Time to feeding cessation (minutes) of S. exigua 3 rd instar larvae exposed to dry insecticide residues on treated tomato leaves Data: P. Marçon & G. Hannig, Delaware, USA

18. DuPont Crop Protection DuPont Rynaxypyr TM Exceptional Crop Protection Rynaxypyr TM 50 GAH Methoxyfenozide 140 GAH E. Benzoate 12 GAH UTC Photos: P. Marçon, Delaware, USA

19. DuPont Crop Protection DuPont Rynaxypyr TM High Insecticidal Potency  Rynaxypyr TM is one order of magnitude more potent against Cydia pomonella, when compared to commonly used commercial standards Comparative Insecticidal Potency of Rynaxypyr TM vs. Commercial Standards against Cydia pomonella neonate larvae (exposure by Ingestion) Data: J. L. Rison et. al., Nambsheim, France

20. DuPont Crop Protection  Rynaxypyr TM has excellent ovi-larvicidal activity  Rynaxypyr TM is a potent larvicide and will control neonates as they ingest the corion to eclode from the egg * Eggs treated with Rynaxypyr TM Intoxicated Neonate attempting to get out of the egg Dead Neonate does not complete eclosion * Anticarsia gemmatalis eggs DuPont Rynaxypyr TM Ovi-Larvicidal Activity Photos: M. Lima, São Paulo, Brazil

21. DuPont Crop Protection DuPont Rynaxypyr TM Rainfastness and Residual Activity  Rynaxypyr TM shows robust rainfastness and residual activity, characteristics which are explained by fundamental biological attributes:  Potent insecticidal activity  Translaminar movement  Rapid feeding cessation  Exceptional crop protection

22. DuPont Crop Protection  Favorable Toxicological and Eco-toxicological profile  Effective and Long-lasting  Crop and Yield protection  Broad Spectrum  Novel mode of action  Excellent crop safety  Soft on beneficial arthropods  Excellent fit into IPM and IRM Programs DuPont Rynaxypyr TM In a nutshell ….

23. DuPont Crop Protection Stanley Royal Sue Rick Glenn Hammes J. Dan Smith Dickie Edmund Eric Castner Charlie Grymes Danny Tamayo Hugo Ramirez Wayne Steele C. Steve Williams Acknowledgments Fred Marmor Paula Marcon Clifton Brister Jay Ellis Dennis Goldsberry

24. DuPont Crop Protection THANK YOU!