1. LSRCP Production Meeting 2011 Re: RY2010 Natural origin adult summer Chinook collection for integrated brood stock production for U.S. v. Oregon

2. ADULT COLLECTION : HOW TO? LOGISTICS & COORDINATION  Collection issues & concepts for capturing natural origin fish (NOR) from the south fork salmon river for IBS production up to 250K smolt  Collect & pond NOR adults @ weir; ISS impact  Collect M & F NOR fish below weir on spawning grounds & transport to holding ponds; practicality & logistics issues  Collect milt from NOR males during trapping and cross with hatchery females  Collect milt from ripe NOR males day of and day before egg take  Coordination & Record Keeping  IDFG Research (Laurie Janssen & crew) and NPT (Rob Hill & Carl East) assisted with data recording (trapping db) and milt collection  Used genetic ID to track male milt to spawn x w/ hatchery female

3. TECHNIQUES & METHODS  Same day & day before spawn take milt collection  Ripe NOR male milt collected, fish passed above weir  Whirl Pac bags were used for collection & labeled w/ genetic ID number  Milt placed in coolers and/or refrigerated if collected the day before egg take  Foot pump was used to add air to the bags to allow the sperm to respire  Paper towel was used to dry the area around the vent  Each NOR male crossed with a single hatchery female  Incubated in trays as singles  No IBS eggs were culled due to high BKD; 381K green collected

4. SPAWN DATA & EYED EGG RESULTS  77 NOR males (x w/ 72 hatchery females) used out of 347 trapped during spawning operations  Natural origin males w/ PITs (7)  3 “Wild” & 4 “Unknown” origin  Same day vs. day before egg take milt collection: IBS eye %  Same day = 88 %  Day before = 69 %  Overall IBS eye % vs. overall hatchery prod eye %  Overall IBS eye % = 77.5 %  Overall hatchery production eye % = 90 %

5.  IBS Prod Numbers  264,000 BY2010 IBS fry on station  Approx 22K excess IBS production mixed w/ 70K hatchery production  All IBS production will be marked as “unclipped CWT only”