1. Supplementation Studies in Idaho Rivers Clear Creek and Pete King Creek (89-098-01) Jill Olson USFWS

2. ISS Study Area (31 streams total) Clearwater Subbasin Salmon Subbasin

3. Research Question #5 Is there an advantage to developing new localized brood stocks with a known natural component for supplementing existing natural populations. Is there an advantage to developing new localized brood stocks with a known natural component for supplementing existing natural populations. 2000 Fish & Wildlife 2000 Fish & WildlifeProgram

4. Implementation: Developing a localized brood stock Kooskia National Fish Hatchery is one of the 7 locations used in the development of localized brood stock for ISS. Kooskia National Fish Hatchery is one of the 7 locations used in the development of localized brood stock for ISS.

5. First generation brood stock (1993-1996) In 1993 adult spring chinook salmon were collected from Kooskia NFH. In 1993 adult spring chinook salmon were collected from Kooskia NFH.

6. First generation brood stock Parr were given a pelvic fin clip so we could distinguish the supplementation outplants from general hatchery production. Parr were given a pelvic fin clip so we could distinguish the supplementation outplants from general hatchery production.

7. First generation brood stock In 1995 smolts with pelvic fin clips were released into Clear Creek. In 1995 smolts with pelvic fin clips were released into Clear Creek. Supplementation with existing hatchery brood stock ended in 1997. Supplementation with existing hatchery brood stock ended in 1997.

8. Second generation brood stock (1997-2002) In 1997 adults from the localized brood stock began to return (BY93). In 1997 adults from the localized brood stock began to return (BY93).

9. Second generation brood stock 60-70% of the natural adults are released above the weir. 60-70% of the natural adults are released above the weir. An equal number of An equal number of supplementation adults are released above weir.

10. Second generation brood stock (30-40%) Natural and some supplementation adults are taken for brood stock. (30-40%) Natural and some supplementation adults are taken for brood stock.

11. Second generation brood stock Natural adults are spawned with other natural adults or supplementation adults. Natural adults are spawned with other natural adults or supplementation adults. Crosses are recorded. Crosses are recorded.

12. Second generation brood stock Eggs are reared to eye-up and transferred back to KNFH. Eggs are reared to eye-up and transferred back to KNFH.

13. Second generation brood stock We began releasing marked supplementation smolts from the localized brood stock in 1999 (BY97). We began releasing marked supplementation smolts from the localized brood stock in 1999 (BY97).

14. ISS Adult Returns to Clear Creek (Return Year 1996-2001)