1. Youngae Lee Identification of microRNA function by target prediction and expression profiling

2. MicroRNA (miRNA) A new family of small noncoding RNA (~22 nt) ~250 miRNA in animal Inhibition of translational (animal) or Degradation of target RNA (plants) Regulation of cell proliferation, differentiation, apoptosis, carcinogenesis and viral infection Unknown function of newly identified animal miRNA

3. The model for biogenesis and post-transcriptional suppression of miRNA and siRNA

4. Purpose Computational target gene prediction Identification of microRNA function Expression profiling

5. Methods 3’-UTR sequence retrieval; ENSEMBL website http:// www. ensembl.org The mature miRNA sequence http://www.sanger.ac.uk/Software/Rfam/mirna/ Identify the targets of miRNAs http://genes.mit.edu/targetscan Gene Ontology (GO) annotations http:// geneontology.org/

6. Results 1. miRNA target prediction algorithm 2. mRNA profiling and function

7. References 1. Azra Krek et al., Nature genetics, 2005, Vol 37, 495 2. Marianthi Kiriakidou et al., Genes and Development, 2006, Vol 18, 1165 3. T. Hubbard et al., Nucleic Acids Research, 2005, Vol 33, D447 4. Victor Ambros. Nature, 2004, Vol 431, 350 5. Xiaowei Wang et al., Nucleic Acids Research, 2006, Vol 34, 1646 6. Benjamin P. Lewis et al., Cell, Vol 115, 787