2. 1400’s Anton van Leeuwenhoek “Father of Microscopy”
Created a Single Lens or “Simple” Microscope
which is curved glass that bends light rays to enlarge an image (has one lens)

3. 1st Compound Microscope
1595, Invented by Zacharias and Hans Janssen
Magnified images using two lens
Total Magnification = 10x

4. Robert Hooke
1665, Discovered cells using a compound miscroscope

5. We Use: Compound Light Microscope
Allows light to pass through the object.
Flat Image

6. Stereoscopic Microscope
Gives a three dimensional (3-D) view of an object. Magnifies 1000x

7. Scanning Electron Microscope
It uses a magnetic field to bend electrons. It magnifies up to 1,000,000 times.

8. Magnification The “power” of a microscope Calculated by multiplying:
the Ocular Lens x the Objective lens

9. Ocular Lens – Type of Lens found in the
eyepiece
Objective Lens – Found on the nosepiece,
range from low to high power

10. What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Total Magnification =
Ocular lens x Objective Lens
- Scanning/Low (4x) = 40x
- Medium (10x) = 100x
- High (40x) = 400x

11. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Field of View – the amount of specimen we are able to see A B
Which of these images would be viewed at a higher power of magnification?

12. Handling a Microscope
Carry the microscope with one hand grasping the arm and the other supporting the base. Hold close to your body.

13. Always have the shortest, low power objective lens facing the stage when you put it away, put a new slide on the stage or remove a slide.

14. Ocular lens (Eyepiece)
Base
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light Source

15. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.