2. Standards and Evaluation http://www.nap.edu/html/nses/overview.ht mlhttp://www.nap.edu/html/nses/overview.ht ml http://ndercbioeyes.wikispaces.com/Align ment+with+Summer+2008+Indiana+K- 12+Science+Standardshttp://ndercbioeyes.wikispaces.com/Align ment+with+Summer+2008+Indiana+K- 12+Science+Standards http://www.doe.state.in.us/standards/welco me2.htmlhttp://www.doe.state.in.us/standards/welco me2.html

3. Assessment

4. “Because if the mother albino has a baby fish by the wildtype there going to see if they have the same DNA” “It is important because you get a better ida by looking in the microscpe.” “The reson why important Because you never know if someone will ask for help in scince.” Classroom Evaluations

5. MicroAgent Multiple Choice #1 Male and female zebrafish have different body shapes. The female has a bigger belly. Which statement best explains why. A.The female finds more food to eat. B.The male gets more exercise by swimming faster. C.The female carries eggs in her egg sac. D.The male fish is younger than the female fish. Using pictures to answer questions. Female ZebrafishMale Zebrafish Best Answer : C

6. Multiple Choice #2 A student would like to see the developing zebrafish inside the egg. Which statement correctly describes the best tool to use for these observations? a. A hand lens will make it possible to view inside the egg. b. A microscope will make it possible to view inside the egg. c. A telescope will allow the egg to be viewed from far away. d. No tool is necessary to view inside the egg. Best Answer: B MicroAgent Multiple Choice #2

7. MicroAgent Open Ended Question #1 Part A. Identify what organ pumps the blood through the body. ___________________________________________ ___________________________________________ ___________________________________________ Blood moves through a fish’s and a human’s body. Part B. Name one thing that gets to the cells through blood. _________________________________________________ _________________________________________________ _________________________________________________

8. Data table for day three development A temperature of 50 ºF is the most effective for hatching zebrafish embryos by day three. A temperature of 65 ºF is more effective than 50 ºF for hatching zebrafish embryos by day three. A temperature of 65 ºF is more effective than 50 ºF and less effective than 90 ºF for hatching zebrafish embryos by day three. Different temperatures have no effect on zebrafish development. TemperatureNumber of Embryos Hatched 50 ºF 45 65 ºF 62 90 ºF 98 Intermediate/Advanced Using Tables

9. Students tested three different temperatures of water to determine if temperature had an effect on the development and hatching of zebrafish embryos from their shells. Three separate groups of zebrafish embryos were allowed to develop for three days. Each group consisted of 100 embryos and was placed in a particular and constant temperature for the three day development. The students recorded the number of hatched fish on day one and on day three. The students had a null hypothesis: Different temperatures will not effect the hatching of zebrafish embryos. Which conclusion is best supported by the data? Intermediate/Advanced Agent Open Ended Question #1

10. Students placed a male and female fish, one with stripes and the other without (albino), in a tank that was left overnight in a warm setting. The next day students removed and observed the tiny eggs to see if any had been fertilized. Next, students placed the embryos and a special fluid in small round plastic dishes. The students counted, cleaned and made observations every day for the following four days. 1. List the names and describe the functions of scientific equipment useful in conducting this activity. 2. How might the students demonstrate what they saw and did each day? Intermediate/Advanced Open Ended Question #2