1. Set up Cornell Notes on pg. 15
Topic: 9.1 Manipulating DNA
Essential Question:
Suppose you cut DNA. You know that you should find four DNA fragments on a gel, but only three appear, and one fragment is very large. Explain what happened?
9.1 Manipulating DNA
2.1 Atoms, Ions, and Molecules
Suppose you cut DNA. You know that you should find four DNA fragments on a gel, but only three appear, and one fragment is very large. Explain what happened?
KEY CONCEPT Biotechnology relies on cutting DNA at specific places

2. P.14

3. Points to Ponder (Top of p. 14)
A natural disaster strikes. Families are separated. How can they be reunited?
If a body is found and the person cannot be identified by looks, how can we identify them?
What types of identifiers do we have?

4. DNA- skin, sperm, saliva, blood Fingerprinting Teeth
A natural disaster strikes. Families are separated. How can they be reunited?
If a body is found and the person cannot be identified by looks, how can we identify them?
What types of identifiers do we have?
DNA- skin, sperm, saliva, blood
Fingerprinting
Teeth

5. Unidentified body found after the sinking of the RMS Titanic known as “The Unknown Child”
NO. 4 - MALE - ESTIMATED AGE, 2 - HAIR, FAIR.
CLOTHING - Grey coat with fur on collar and cuffs; brown serge frock; petticoat; flannel garment; pink woolen singlet; brown shoes and stockings.
No marks whatever.
Possibly third-class.[3]

6. The sailors aboard the Mackay-Bennett, who were very upset by the discovery of the unknown boy's body, paid for a monument, and he was buried on 4 May 1912 with a copper pendant placed in his coffin by recovery sailors that read "Our Babe."

7. His body, identified as that of a child around two years old, was initially believed to be that of either a two-year-old Swedish boy, Gösta Pålsson; a two-year-old Irish boy, Eugene Rice,  or Eino Viljami Panula, a 13-month old Finnish baby
However, with improved DNA testing available in 2007, Canadian researchers at Lakehead University in Thunder Bay tested the child's HVS1, a type of mitochondrial DNA molecule, and it did not match the Panula family.

8. Finally identified in 2007 using DNA evidence
DNA extracted from the exhumed remains and DNA provided by a surviving maternal relative helped positively match the remains to Sidney Leslie Goodwin ( ), and the reidentification was announced on 30 July 2007
The Goodwins and their 6 children were third class passengers and all perished

9. KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
Baby 81 was rescued after the 2004 Indian Ocean tsunami. He was reunited with his parents by using DNA fingerprinting technology

10. Biotechnology allows us to
Produce transgenic organisms
Clone
Study diseases and evolution
Produce medical treatments for people with illnesses

11. DNA cannot be picked up and rearranged by hand
Therefore, scientists must be able to work with DNA without being able to see it, or handle it directly

12. Scientists use several techniques to manipulate DNA.
Chemicals, computers, and bacteria are used to work with DNA.
Scientists use these tools in genetics research and biotechnology.

13. Restriction enzymes act as “scissors” by cutting DNA
allow scientists to more easily study and manipulate genes
cut DNA at a specific nucleotide sequence called a restriction site
DNA
Restriction enzyme

14. Different restriction enzymes cut DNA in different ways.
each enzyme has a different restriction site

15. some cut straight across and leave “blunt ends”
some make staggered cuts and leave “sticky ends”
Sticky ends

16. Between what nucleotides does this restriction enzyme cut between?
T and C or C and T

17. ATTACGACCTAGGACG 1. How many fragments are produced?
Restriction Enzymes (Middle of p. 14)
Restriction enzyme: cuts between T and A
ATTACGACCTAGGACG
1. How many fragments are produced?
2. Are all the fragments the same length?
3. Please organize the fragments from biggest to smallest.
4. How many nucleotides in each segment when organized?

18. ATTACGACCTAGGACG How many fragments are produced? 3
Restriction enzyme: cuts between T and A
ATTACGACCTAGGACG
How many fragments are produced? 3
Are all the fragments the same length? No
Please organize the fragments from biggest to smallest. ACGACCT/AGGACG/ATT
How many nucleotides in each segment when organized? 7, 6, 3

19. Restriction Enzyme Video 2m39s

20. After DNA has been cut, several different things can be done with it:
The gene can be studied
The gene can be placed in another organism
But first, the DNA fragments have to be separated from one another
Sorted according to their size

21. Gel electrophoresis is used to separate DNA fragments by size.
A DNA sample is cut with restriction enzymes
Electrical current pulls DNA fragments through a gel

22. Smaller fragments move faster and travel farther than larger fragments
Fragments of different sizes appear as bands on the gel

23. A restriction map shows the lengths of DNA fragments between restriction sites.
only indicate size, not DNA sequence
useful in genetic engineering
used to study mutations

24. ATTACGACCTAGGACG Restriction enzyme: cuts between T and A
Restriction Enzymes (Middle of p. 14)
Restriction enzyme: cuts between T and A
ATTACGACCTAGGACG
You organized the fragments from biggest to smallest.
ACGACCT (7)
AGGACG (6)
ATT (3)

25. -
Grab 3 colors. Please organize the fragments from biggest to smallest on this restriction map.
ACGACCT (7)
AGGACG (6)
ATT (3) 10 9 8 7 6 5 4 3 2 1 - - -
Draw and complete this restriction map on the bottom of pg. 14 +

26. “Who Ate the Cheese?” Warm-Up
Please complete the warm-up by yourself
Books open to
5 mins

27. Who Ate the Cheese? Practice Gel Electrophoresis-Restriction Enzyme Lab
Objective: You will examine crime evidence and model the process of gel electrophoresis and DNA fingerprinting to identify the person who ate the Queen’s cheese.
Read the Royal Incident Report
You are looking for the sequence CCGG
GG CC
The restriction enzyme always cuts between the C /G
All cuts will leave blunt ends
Count the number of base pairs in each fragment of DNA
Record the # on the bottom of each DNA fragment
Make a restriction map to identify who, if anyone, is guilty of eating the queen’s cheese