1. Biotechnology Rauful Hossain Leutrim Cahni

3. Recombinant DNA Gel Electrophoresis PCR Plasmid

6. Recombinant DNAGene Manipulation PCRTransformation/ Transfection Applications in the world To cut up certain, specific DNA sequences in an attempt to separate them for evaluation Restriction EnzymesCollection of DNA fragmentsDNA primers added for replication Recombinant DNA inserted into host cell Used as innovations to improve lifestyle To make it easier to observe the DNA through the gel Same sequences read from opposite sides Its size DNA or RNA molecule that acts as starting point for 3 prime chain growth Electroporation, Chemically treating the cell It can recognize DNA sequences To separate the DNA strands based on length and size Molecules that bond and carry foreign DNA to cells Selectable marker genesDNA templateOpens up the membrane pores to let in the vector Vaccines contain a viral strand of DNA that has been made non lethal or weakened- through removing genes. How long certain gene sequences are and whose DNA matches DNA ligasecDNARNA transcriptaseNormal vectors don’t have genes supporting the expressing of those genes Health risks, not natural, and obstruction with destiny. This is when Biologists or forensic specialists analyze a gel electrophoresis experiment. Plasmids contain no introns, which cause many disruptions to gene addition. But, eukaryotic DNA contains plenty. Stem cells, can be transformed with recombinant DNA denaturation, annealing, elongation Transgenic AnimalThe gene coding for insulin wouldn’t have been able to be transfected into our somatic cells 200 400 600 800 1000

8. Why use restriction enzymes? What enzymes are used to cut DNA sequences? What is produced to cut DNA molecules? What is a genomic library?What is a vector?What is biotech generally used for? Why use a dye on the DNA?What is a palindrome?What makes E.coli easy to manipulate? What is a primer?What are methods to insert DNA into host cells? What can electrophoresis do in criminal investigations? What is the role of the electrical current running through the gel? What are vectors?What genes confer resistance to antibodies? What is targeted by the PCR? What does the heat shock do to the host cell? How are vaccines made? What can seeing how DNA separates lead you to conclude? Which enzyme is used to bind foreign DNA to a vector plasmid What can be used to make genes transcribed to a particular tissue? What is not required in PCR reactions? Why aren’t normal vectors able to be used in transfection? What ethical issues are anti- biotech? What are RFLPs?How are plant cells good hosts for recombinant DNA? What are the 3 main steps in a PCR cycle? What is an animal that has gained new genetic information from foriegn DNA Without biotech, why would diabetes be a bigger problem in the world?