1. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? -Phospholipid bilayer -Selectively permeable -Fluid Mosaic Model Hydrophilic head Hydrophobic tail WATER

2. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? -Phospholipid bilayer -Selectively permeable -Fluid Mosaic Model 2.Why fluid/what affects fluidity? -FA - Sat vs unsaturated -Temperature - kinetics -Cholesterol Lateral movement (~10 7 times per second) Flip-flop (~ once per month) Fluid Viscous Unsaturated hydrocarbon tails with kinks Saturated hydro- Carbon tails (a) Movement of phospholipids (b) Membrane fluidity (c) Cholesterol within the animal cell membrane Cholesterol Rarely!!

3. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? -Phospholipid bilayer -Selectively permeable -Fluid Mosaic Model 2.Why fluid/what affects fluidity? -FA - Sat vs unsaturated -Temperature – kinetics -Cholesterol 3. What makes a membrane mosaic?

4. Students -Transport – TODAY -1 bag with handles tied -Take to the 1 st floor of near stairwell -Behind the taped line -Thank you!! -Ecology test math to show me? -LL check – Thursday – only Chapter 6 -Cell phones in bin – please & thank you

5. Figure 7.7 The detailed structure of an animal cell’s plasma membrane Glycoprotein Carbohydrate Microfilaments of cytoskeleton Cholesterol Peripheral protein Integral protein CYTOPLASMIC SIDE OF MEMBRANE EXTRACELLULAR SIDE OF MEMBRANE Glycolipid Fibers of extracellular matrix (ECM)

6. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? -Phospholipid bilayer -Selectively permeable -Fluid Mosaic Model 2.Why fluid/what affects fluidity? -FA - Sat vs unsaturated -Temperature – kinetics -Cholesterol 3.What makes a membrane mosaic? 4.How are integral proteins held in the membrane? - α-helix EXTRACELLULAR SIDE N-terminus C-terminus  Helix CYTOPLASMIC SIDE

7. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? 2.Why fluid/what affects fluidity? 3.What makes a membrane mosaic? 4.How are integral proteins held in the membrane? 5.What are the functions of membrane proteins?

8. Figure 7.9 Some functions of membrane proteins Transport. (left) A protein that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a particular solute. (right) Other transport proteins shuttle a substance from one side to the other by changing shape. Some of these proteins hydrolyze ATP as an energy source to actively pump substances across the membrane. Enzymatic activity. A protein built into the membrane may be an enzyme with its active site exposed to substances in the adjacent solution. In some cases, several enzymes in a membrane are organized as a team that carries out sequential steps of a metabolic pathway. Signal transduction. A membrane protein may have a binding site with a specific shape that fits the shape of a chemical messenger, such as a hormone. The external messenger (signal) may cause a conformational change in the protein (receptor) that relays the message to the inside of the cell. (a) (b) (c) ATP Enzymes Signal Receptor

9. (d) (e) Cell-cell recognition. Some glyco-proteins serve as identification tags that are specifically recognized by other cells. Intercellular joining. Membrane proteins of adjacent cells may hook together in various kinds of junctions, such as gap junctions or tight junctions (see Figure 6.31). Attachment to the cytoskeleton and extracellular matrix (ECM). Microfilaments or other elements of the cytoskeleton may be bonded to membrane proteins, a function that helps maintain cell shape and stabilizes the location of certain membrane proteins. Proteins that adhere to the ECM can coordinate extracellular and intracellular changes (see Figure 6.29). (f) Glyco- protein

10. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? 2.Why fluid/what affects fluidity? 3.What makes a membrane mosaic? 4.How are integral proteins held in the membrane? 5.What are the functions of proteins? 6.Word association…… Membrane = 7. What things are selected for, i.e. what can pass easily? -Non-polar – steroids, O 2, CO 2 8.What things are selected against? (met with resistance by polar heads) -Large & polar, or charged molecules – glucose, amino acids, ions 9.What is the difference between hypertonic & hypotonic solutions? -Hypertonic – more total solute than another sol’n = less water -Hypotonic – less total solute than another sol’n = more water Selectively permeable!!!!

11. Figure 7.11 The diffusion of solutes across a membrane (a) Diffusion of one solute. The membrane has pores large enough for molecules of dye to pass through. Random movement of dye molecules will cause some to pass through the pores; this will happen more often on the side with more molecules. The dye diffuses from where it is more concentrated to where it is less concentrated (called diffusing down a concentration gradient). This leads to a dynamic equilibrium: The solute molecules continue to cross the membrane, but at equal rates in both directions. Diffusion of two solutes. Solutions of two different dyes are separated by a membrane that is permeable to both. Each dye diffuses down its own concen- tration gradient. There will be a net diffusion of the purple dye toward the left, even though the total solute concentration was initially greater on the left side. (b) Osmosis Molecules of dye Membrane (cross section) WATER Net diffusion Equilibrium Net diffusion Equilibrium What is osmosis? - Diffusion of water

12. Figure 7.12 Osmosis Lower concentration of solute (sugar) Higher concentration of sugar Same concentration of sugar Selectively permeable mem- brane: sugar mole- cules cannot pass through pores, but water molecules can More free water molecules (higher concentration) Water molecules cluster around sugar molecules Fewer free water molecules (lower concentration) Water moves from an area of higher free water concentration to an area of lower free water concentration  Osmosis What about transport in actual cells?

13. Figure 7.13 The water balance of living cells Hypotonic solutionIsotonic solution Hypertonic solution Animal cell. An animal cell fares best in an isotonic environ- ment unless it has special adaptations to offset the osmotic uptake or loss of water. Plant cell. Plant cells are turgid (firm) and generally healthiest in a hypotonic environ- ment, where the uptake of water is eventually balanced by the elastic wall pushing back on the cell. (a) (b) H2OH2OH2OH2O H2OH2OH2OH2O H2OH2OH2OH2O H2OH2O H2OH2O Lysed NormalShriveled Turgid (normal)Flaccid Plasmolyzed Placed into

14. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? 2.Why fluid/what affects fluidity? 3.What makes a membrane mosaic? 4.How are integral proteins held in the membrane? 5.What are the functions of proteins? 6.Word association…… 7. What things are selected for? 8.What things are selected against? 9.What is the difference between hypertonic & hypotonic solutions? -Hypertonic – more total solute than another sol’n -Hypotonic – less total solute than another sol’n 10. How are substances transported across membranes? -Passive transport – no energy expended & things flow down concentration gradient from high to low -Simple diffusion -Facilitated diffusion -Active transport – energy required & things flow AGAINST a concentration gradient from low to high

15. Figure 7.17 Review: passive and active transport compared Passive transport. Substances diffuse spontaneously down their concentration gradients, crossing a membrane with no expenditure of energy by the cell. The rate of diffusion can be greatly increased by transport proteins in the membrane. Active transport. Some transport proteins act as pumps, moving substances across a membrane against their concentration gradients. Energy for this work is usually supplied by ATP. ATP Diffusion. Hydrophobic molecules and (at a slow rate) very small uncharged polar molecules can diffuse through the lipid bilayer. Facilitated diffusion. Many hydrophilic substances diffuse through membranes with the assistance of transport proteins, either channel or carrier proteins.

16. Chapter 7: Membrane Structure & Function 1.What do you know about membranes? 2.Why fluid/what affects fluidity? 3.What makes a membrane mosaic? 4.How are integral proteins held in the membrane? 5.What are the functions of proteins? 6.Word association…… 7. What things are selected for? 8.What things are selected against? 9.What is the difference between hypertonic & hypotonic solutions? 10. How are substances transported across membranes? -Passive transport – no energy expended & things flow down concentration gradient -Simple diffusion -Facilitated diffusion -Active transport – energy required & things flow AGAINST a concentration gradient 11. What are some other mechanisms of transport across membranes?

17. Figure 7.20 Exploring Endocytosis in Animal Cells EXTRACELLULAR FLUID In phagocytosis, a cell engulfs a particle by wrapping pseudopodia around it and packaging it within a membrane- enclosed sac large enough to be classified as a vacuole. The particle is digested after the vacuole fuses with a lysosome containing hydrolytic enzymes. Pseudopodium CYTOPLASM “Food” or other particle Food vacuole 1 µm Pseudopodium of amoeba Bacterium Food vacuole An amoeba engulfing a bacterium via phagocytosis (TEM). PHAGOCYTOSIS PINOCYTOSIS Pinocytosis vesicles forming (arrows) in a cell lining a small blood vessel (TEM). 0.5 µm In pinocytosis, the cell “gulps” droplets of extracellular fluid into tiny vesicles. It is not the fluid itself that is needed by the cell, but the molecules dissolved in the droplet. Because any and all included solutes are taken into the cell, pinocytosis is nonspecific in the substances it transports. Plasma membrane Vesicle

18. RECEPTOR-MEDIATED ENDOCYTOSIS Receptor Ligand Coat protein Coated pit Coated vesicle A coated pit and a coated vesicle formed during receptor- mediated endocytosis (TEMs). 0.25 µm Plasma membrane Coat protein Receptor-mediated endocytosis enables the cell to acquire bulk quantities of specific substances, even though those substances may not be very concentrated in the extracellular fluid. Embedded in the membrane are proteins with specific receptor sites exposed to the extracellular fluid. The receptor proteins are usually already clustered in regions of the membrane called coated pits, which are lined on their cytoplasmic side by a fuzzy layer of coat proteins. Extracellular substances (ligands) bind to these receptors. When binding occurs, the coated pit forms a vesicle containing the ligand molecules. Notice that there are relatively more bound molecules (purple) inside the vesicle, but other molecules (green) are also present. After this ingested material is liberated from the vesicle, the receptors are recycled to the plasma membrane by the same vesicle.

19. Students -Take out LL for check -Final observations of demo -Help…..sigh…. -Cell phones in bin….please & thank you! Lab resources Sucrose solutions Water Cups or beakers Dialysis tubing Graduated cylinders & funnels or pipets Scales Paper towels

20. 1.Hypothesis – “The hypothesis to be tested is…..” 2.Independent & dependent variables - IV: experimenter manipulates DV: what is measured 3.Control group & experimental group 4.Constants – same temp., same volume, equipment, time - “The constants are….” 5.Controls – no enzyme, no substrate, dark conditions 6.Random sampling 7.Large sample size (n) 8.Repeated – multiple trials 9.Graphs 1.Label axes w/ units 2.Keep same scale 3.Title 4.Start at X & Y vertex 5.Connect dots 6.Key – if more than one line Good Experimental Design

21. 1.Hypothesis – “The hypothesis to be tested is…..” 2.Independent & dependent variables - IV: sucrose sol’ns DV: % change in mass 3.Control group: Experimental group: 4.Constants: -same temp – -same volume – -equipment – -Time – 5.Controls – 6.Random sampling – N/A 7.Large sample size – 8.Repeated – 9.Graph When massing bag, blot off outside. Only inside contents matter. Yes, you may use your phone as a timer. Good Experimental Design H2OH2OA - E RT 10 mL in bag…..excess of H 2 O in cup scales, pipets, 25 min? H 2 O sample classes all day % change = F-I x 100% I

22. Students -Get test folder -Grades updated later today -Sit with lab groups…..as much as possible….2-3 member groups on “T” -Pull out osmosis data…..calculate % change -Phones in bins – mute or off – please & thank you -Upcoming deadlines…. -F & M: Enzyme lab….finish these labs by Thursday!!! -T – W: Test -Th (10/23): Labs due