1. 1 GROWTH AND CULTURING OF BACTERIA CHAPTER 6

2. 2 Growth and Cell Division Growth Binary fission

3. 3

4. 4 Cell Division Budding

5. 5 Bacterial Growth Curve Lag phase Log phase Stationary phase Death phase

6. 6 Measuring Bacterial Growth Serial dilution/ Standard plate counts

7. 7 Measuring Bacterial Growth Direct microscopic counts

8. 8 Measuring Bacterial Growth Most probable number

9. 9 Measuring Bacterial Growth Filtration Turbidity (Spectrophotometric)

10. 10 Filtration

11. 11 Turbidity

12. 12 Physical Factors Affecting Bacterial Growth pH Temperature

13. 13 Oxygen Moisture Physical Factors Affecting Bacterial Growth

14. 14 Hydrostatic pressure Osmotic pressure Radiation Physical Factors Affecting Bacterial Growth

15. 15 Radiation: Ionizing (gamma radiation) Ultraviolet radiation: DNA absorbs 260 nm light “Thymine dimers”

16. 16 Nutritional Factors Affecting Bacterial Growth Carbon source Nitrogen source NH 4 best, but NO 3 and NO 2 can work too. Sulfur/ phosphorus PO 4 3- SO 4 2- C 6 H 12 O 6 best but many sugars CO 2 for autotrophs

17. 17 Nutritional Factors Affecting Bacterial Growth Trace elements Vitamins Nutritional complexity

18. 18

19. 19 Locations of Enzymes Extracellular Periplasmic kinases and phosphatases Cytoplasmic Proteases and cellulases (hydrolases) Foldases, dehydrogenases

20. 20 Sporulation Endospores Other sporelike bacterial structures

21. 21

22. 22 Bacillus subtilis spore stain

23. 23 Culturing Bacteria — Streak Plates Streak plate method

24. 24 Culturing Bacteria — Pour Plates Pour plate method

25. 25 Culture Media Types –Defined –Complex LB (Luria-Bertani) TSA

26. 26 Culture Media Purposes Selective media Differential media Enrichment media McConkey’s

27. 27 Detection of Carbon source fermenters McConkey’s agar

28. 28 Culture Conditions — O 2 Controlling oxygen content

29. 29 Culture conditions Maintaining cultures Preserved cultures Diagnostic tests Limitations

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