1. Chapter 6: Microbial Growth
Growth of Bacterial Cultures
Physical Requirements
Chemical
Requirements
Measurement of Growth
Culture Media
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FINAL ROUND

2. Topic 1: Physical Requirements $100 Question
The optimum pH for most bacteria is 1. 5. 7. 9.
Answer = C
ANSWER
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3. Topic 1: Physical Requirements $100 Answer
The optimum pH for most bacteria is 1. 5. 7. 9.
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4. Topic 1: Physical Requirements $200 Question
A psychrophile grows best at
37°C.
10°C.
25°C.
15°C.
Answer = B
ANSWER
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5. Topic 1: Physical Requirements $200 Answer
A psychrophile grows best at
37°C.
10°C.
25°C.
15°C.
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6. Topic 1: Physical Requirements $300 Question
Organisms that require high salt concentrations for growth are called
halophiles.
extreme halophiles.
facultative halophiles.
microhalophiles.
Answer = B
ANSWER
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7. Topic 1: Physical Requirements $300 Answer
Organisms that require high salt concentrations for growth are called
halophiles.
extreme halophiles.
facultative halophiles.
microhalophiles.
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8. Topic 1: Physical Requirements $400 Question
What is the energy source utilized by producers in the hydrothermal vents on the
ocean floor?
light
hydrogen sulfide
organic molecules
carbon dioxide
Answer = B
ANSWER
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9. Topic 1: Physical Requirements $400 Answer
What is the energy source utilized by
producers in the hydrothermal vents on the
ocean floor?
light
hydrogen sulfide
organic molecules
carbon dioxide
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10. Topic 1: Physical Requirements $500 Question
In food preservation, salts and sugars are
used because they create a
lower pH.
hypertonic environment.
hypotonic environment.
lower osmotic pressure.
Answer = B
ANSWER
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11. Topic 1: Physical Requirements $500 Answer
In food preservation, salts and sugars are
used because they create a
lower pH.
hypertonic environment.
hypotonic environment.
lower osmotic pressure.
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12. Topic 2: Chemical Requirements $100 Question
Chemoautotrophs and photoautotrophs derive their carbon from
carbon dioxide.
proteins.
lipids.
carbohydrates.
Answer = A
ANSWER
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13. Topic 2: Chemical Requirements $100 Answer
Chemoautotrophs and photoautotrophs derive their carbon from
carbon dioxide.
proteins.
lipids.
carbohydrates.
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14. Topic 2: Chemical Requirements $200 Question
Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
chemosynthesis.
photosynthesis.
nitrogen fixation.
nitrogen synthesis.
Answer = C
ANSWER
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15. Topic 2: Chemical Requirements $200 Answer
Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
chemosynthesis.
photosynthesis.
nitrogen fixation.
nitrogen synthesis.
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16. Topic 2: Chemical Requirements $300 Question
An organism that uses oxygen but can grow without oxygen is called a(n)
obligate aerobe.
facultative aerobe.
aerobe.
aerotolerant anaerobe.
Answer = B
ANSWER
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17. Topic 2: Chemical Requirements $300 Answer
An organism that uses oxygen but can grow without oxygen is called a(n)
obligate aerobe.
facultative aerobe.
aerobe.
aerotolerant anaerobe.
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18. Topic 2: Chemical Requirements $400 Question
Which organism is killed by atmospheric oxygen?
aerobe
obligate aerobe
obligate anaerobe
aerotolerant anaerobe
Answer = C
ANSWER
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19. Topic 2: Chemical Requirements $400 Answer
Which organism is killed by atmospheric
oxygen?
aerobe
obligate aerobe
obligate anaerobe
aerotolerant anaerobe
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20. Topic 2: Chemical Requirements $500 Question
Biofilms reside in a matrix made up primarily of
lipids.
hydrogel.
proteins.
polysaccharides.
Answer = D
ANSWER
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21. Topic 2: Chemical Requirements $500 Answer
Biofilms reside in a matrix made up primarily of
lipids.
hydrogel.
proteins.
polysaccharides.
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22. Topic 3: Growth of Bacterial Cultures $100 Question
Generation time can best be defined as the
length of time it takes for lag phase.
duration of log phase.
length of time it takes for a cell to divide.
minimum rate of doubling.
Answer = C
ANSWER
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23. Topic 3: Growth of Bacterial Cultures $100 Answer
Generation time can best be defined as the
length of time it takes for lag phase.
duration of log phase.
length of time it takes for a cell to divide.
minimum rate of doubling.
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24. Topic 3: Growth of Bacterial Cultures $200 Question
How many cells would be present after four
generations if we started with three cells? 12 24 48 96
Answer = C
ANSWER
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25. Topic 3: Growth of Bacterial Cultures $200 Answer
How many cells would be present after four
generations if we started with three cells? 12 24 48 96
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26. Topic 3: Growth of Bacterial Cultures $300 Question
In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?
stationary phase
log phase
death phase
lag phase
Answer = D
ANSWER
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27. Topic 3: Growth of Bacterial Cultures $300 Answer
In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium?
stationary phase
log phase
death phase
lag phase
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28. Topic 3: Growth of Bacterial Cultures $400 Question
Which of the following is NOT a possible reason why exponential growth stops?
accumulation of waste products
exhaustion of nutrients
harmful changes in pH
All of these are possible reasons.
Answer = D
ANSWER
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29. Topic 3: Growth of Bacterial Cultures $400 Answer
Which of the following is NOT a possible reason why exponential growth stops?
accumulation of waste products
exhaustion of nutrients
harmful changes in pH
All of these are possible reasons.
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30. Topic 3: Growth of Bacterial Cultures $500 Question
In the stationary phase,
the number of microbial deaths exceeds the number of new cells formed.
the number of microbial deaths is equal to the number of new cells formed.
new cells are no longer formed because of adverse environmental conditions.
cellular reproduction is most active.
Answer = B
ANSWER
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31. Topic 3: Growth of Bacterial Cultures $500 Answer
In the stationary phase,
the number of microbial deaths exceeds the number of new cells formed.
the number of microbial deaths is equal to the number of new cells formed.
new cells are no longer formed because of adverse environmental conditions.
cellular reproduction is most active.
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32. Topic 4: Culture Media $100 Question
Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?
selective media
complex media
selective and differential media
enrichment media
Answer = C
ANSWER
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33. Topic 4: Culture Media $100 Answer
Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms?
selective media
complex media
selective and differential media
enrichment media
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34. Topic 4: Culture Media $200 Question
The microbes that grow and multiply in or on a culture medium are referred to as a(n)
culture.
inoculum.
specimen.
medium.
Answer = A
ANSWER
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35. Topic 4: Culture Media $200 Answer
The microbes that grow and multiply in or on a culture medium are referred to as a(n)
culture.
inoculum.
specimen.
medium.
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36. Topic 4: Culture Media $300 Question
What can be added to a medium when it is desirable to grow bacteria on a solid medium?
glucose
magnesium sulfate
potassium phosphate
agar
Answer = D
ANSWER
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37. Topic 4: Culture Media $300 Answer
What can be added to a medium when it is desirable to grow bacteria on a solid medium?
glucose
magnesium sulfate
potassium phosphate
agar
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38. Topic 4: Culture Media $400 Question
A culture medium consisting of agar, human blood, and beef heart is a _____ medium.
complex
selective
differential
chemically defined
Answer = A
ANSWER
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39. Topic 4: Culture Media $400 Answer
A culture medium consisting of agar, human blood, and beef heart is a _____ medium.
complex
selective
differential
chemically defined
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40. Topic 4: Culture Media $500 Question
The source of nutrients in nutrient agar is
agar.
peptone and sodium chloride.
peptone and beef extract.
nutrient.
Answer = C
ANSWER
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41. Topic 4: Culture Media $500 Answer
The source of nutrients in nutrient agar is
agar.
peptone and sodium chloride.
peptone and beef extract.
nutrient.
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42. Topic 5: Measurement of Growth $100 Question
A spectrophotometer can be used to measure
metabolic activity.
turbidity.
dry weight.
pathogenicity.
Answer = B
ANSWER
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43. Topic 5: Measurement of Growth $100 Answer
A spectrophotometer can be used to measure
metabolic activity.
turbidity.
dry weight.
pathogenicity.
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44. Topic 5: Measurement of Growth $200 Question
Because of time considerations, which method is usually used to count the number of bacteria in milk?
the direct microscope count method
the pour plate method
the spread plate method
the most probable number method
Answer = A
ANSWER
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45. Topic 5: Measurement of Growth $200 Answer
Because of time considerations, which method is usually used to count the number of bacteria in milk?
the direct microscope count method
the pour plate method
the spread plate method
the most probable number method
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46. Topic 5: Measurement of Growth $300 Question
Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?
the most probable number method
serial dilution
filtration
spread plate method
Answer = B
ANSWER
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47. Topic 5: Measurement of Growth $300 Answer
Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?
the most probable number method
serial dilution
filtration
spread plate method
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48. Topic 5: Measurement of Growth $400 Question
Which is the best technique to use to measure the growth of filamentous organisms?
most probable number method
direct microscopic count
turbidity
dry weight
Answer = D
ANSWER
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49. Topic 5: Measurement of Growth $400 Answer
Which is the best technique to use to measure the growth of filamentous organisms?
most probable number method
direct microscopic count
turbidity
dry weight
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50. Topic 5: Measurement of Growth $500 Question
Which technique is frequently used to detect and enumerate coliform bacteria?
turbidity
spread plate method
filtration
pour plate method
Answer = C
ANSWER
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51. Topic 5: Measurement of Growth $500 Answer
Which technique is frequently used to detect and enumerate coliform bacteria?
turbidity
spread plate method
filtration
pour plate method
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52. FINAL ROUND Question
An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? 32 6 4 5
Answer = D
ANSWER
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53. FINAL ROUND Answer
An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? 32 6 4 5
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