2. Dr. Mariam Eshak 2015 Expression of Inflammatory and Cell Death Genes and DNA Damage Induced by Endotoxic Shock in Laying Hens Mariam G. Eshak 1, Ahmed Abbas 2, M. I. El-Sabry 2 and M. M. Mashaly 2,3 1 Department of Cell Biology, National Research centre, Giza, Egypt. 2 Animal production Department, Cairo University, Giza, Egypt. 3 Animal and Poultry Science Department, Pennsylvania State University, USA. Title

3. Dr. Mariam Eshak 2015 Low egg production High Mortality ? How Objective This investigation was conducted to determine the physiological response and evaluate the expression of inflammatory and cell death genes and DNA damage induced by endotoxic shock in laying hens. Challenge

4. Dr. Mariam Eshak 2015 Experimental Design Control E. Coli 120 birds (10 7 colonies/shut) 35 wk of age 40 H&N Gene expression analysis Real Time PCR DNA damageComet assay 40

5. Dr. Mariam Eshak 2015 Results

6. Dr. Mariam Eshak 2015 TreatmentTissuesNumber of animals No. of cells Class of comet DNA damaged cells (%) Analyzed Total comets 0123 Control Brain5500214791650 4.2 E.coli 550043457111418 8.6 Control Liver5500234771940 4.6 E.coli 550056444102224 11.2 Stages of DNA Damage 0 1 23 Conclusion Introducing E. Coli to hens induced inflamm- atory physiological response and triggers cell death program by promoting P38, IL-1β, and TNF-α gene expression in the brain and liver. Moreover, The DNA damage % was higher in liver more than brain tissue Introducing E. Coli to hens induced inflamm- atory physiological response and triggers cell death program by promoting P38, IL-1β, and TNF-α gene expression in the brain and liver. Moreover, The DNA damage % was higher in liver more than brain tissue