1. Human Physiology ISA – ISA 1 Amylase Action

2. Enzyme Reactions

3. Variables Independent Dependent Control Variables Temperature
Time it takes to digest starch
Control Variables
Volume of starch/iodine/water/amylase
Concentration of starch/amylase

4. Apparatus 1% amylase 0.5% starch Strong iodine Thermometers
Spotting tiles
Tin foil
Pipettes

5. Investigation- Independent Variable – Temperature
Investigation- Independent Variable – Temperature. Dependent Variable- time taken for starch to digest
To one test tube add 4cm3 of starch solution
To another test tubes add 1cm3 of amylase
Set up a water bath to your required temperature (20,30,40,50,60)
Place both test tubes in the water bath for 5 minutes.
During the 5 minutes set up a white tile with a drop of iodine in each well – each well represents 30 seconds.
After 5 minutes pour the starch in to the amylase test tube.
Start timing
Take a small sample and place in the first well
Place back in the water bath
Take a sample from the test tube every 30 seconds and add to an iodine drop in the well.
Continue until the colour no longer changes
Repeat for different temperatures e

6. Results – Plot a graph Temperature © Time taken to digest starch (s)20
210 25
150 30 90 35 40 45
180 50
330 55
600

7. Investigation What was the Control Investigation and why? Reliability
Conclusion – include figures

8. Design an Investigation
Think about variables
Think about what you are changing – the temperature
Think about what you need to keep the same
Think about how you will know which one has worked
Think about repeats