1. Harvesting Algae to Form a Neutraceutical, Specifically Creating a Functional Food Team Alpha Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune Otterson Meskyte Charu Saini Crypthecodinium Cohnii

2. Outline Flow Sheeting Energy Sinks/Loads and Streams Equipment Sizing Costing

3. Degumming Bleaching Removal FFA Deodorization Centrifuge Crude SBO Citric Acid Gum Water Citric Acid Acid Activated Clay Enzyme Water Acid Activate Clay Interesterification Hardfat SBO EmulsificationPasteurization Water Sea Salt Lecithin Diacetyl Mono/Diglycerides B-Carotenes Sorbic Acid Ergocaliferols Sodium Hydroxide Citric Acid Water Sodium Hydroxide Citric Acid Water Scraped Surface Heat Exchanger Packaging Refrigerant To Wholesaler Dissolved Air Floatation FermentationCentrifuge CO 2 Lysing Glucose Beer Still Bottom Antifoam Water Algae Glucanex Steam Cooling Water Sterile air Vent Water Air CO 2 LHO- Biomass Water NaOH Steam Salt CIP System

4. Fermentor PFD

5. 9 12 8 6 11 13 DAF PFD 10 14 Rxn tank A Rxn tank B 18 15 16 17 19 18 22 21 20 P-01 P-02 P-03 21 14 23 Fermented Algae Fermented Algae Fermented Algae Sterilized Air Vented Air Conc. Algae Waste Water Conc. Algae 18 Sterilized Air

6. 22 23 24 1 Baffle tray Tower Lysed CO2 Holding tank CO2 Lysing PFD 24 23 25 24 26 27 28 Conc. Algae Sterilized CO2 CO2 Absorbed Algae Lysed Algae & CO2 Vented CO2 Lysed Algae Throttle Valve

7. 3 Main Centrifuge PFD 13 31 29 30 Centrifuge Lysed Algae Biomass Algae Oil

8. TK- 01 Crude SBO Storage P-01 54 32 N 2 Gas 1 6 TK-02 45% Citric Acid Storage P-02 7 8 9 10 P-03P-04P-05 TK-03 4% NaOH Storage TK-04 Water Storage TK-05 10% Enzyme Refrigerated Storage 1211 13 14 N 2 Gas Cooling Heating 2322 1516 HLO - Gums 19 TK-06 Batch Reactor P-06P-07 TK-07 Surge Tank 20 24 N 2 Gas TK-08 Degummed SBO Storage Heating HX-1 HX-3 LLO 21 1817 HX-2 Cent-01 Degumming PFD

9. 24 N 2 Gas TK-08 Degummed SBO Storage 2827 HX-4 2526 TK-09 50% Citric Acid Storage 29 P-08 P-09 30 TK-10 Acid Activated Clay Tank 32 31 P-10 33 P-11 TK-11 Water Storage 34 35 36 38 37 P-12 HX-5 40 39 P-13 VP-1 TK-12 Mixer 42 41 43 N 2 Gas TK-13 Surge Tank 44 P-14 45 47 48 46 MX-2MX-1 49 VP-2 50 51 52 53 54 Steam 55 5657 58 To MX-1 P-15 59 60 6263 64 N 2 Gas 61 TK-14 Refined SBO Storage HX-6 HX-7 HX-8 HX-9 Filter Vacuum Vessel 3837 Refining PFD

10. 64 N 2 Gas TK-14 Refined SBO Storage 65 N 2 Gas TK-15 SBO Hardfat Storage 67 N 2 Gas TK-16 SBO Hardfat Heating Tank 66 68 69 70 74 7372 7776 71 75 P-17 P-18 HX-10 HX-11 78 VP-3 79 80 81 P-19 TK-17 CH 3 ONa Storage 82 TK-19 Mixing Tank P-20 85 P-21 Filter 86 89 90 8788 91 9293 94 TK-20 Soft Water Storage HX-12 HLO P-22 P-23P-24 95 HLO 96 Cent-03Cent-02 TK-18 20% Citric Acid Storage TK-21 Dryer 97 98 P-25 2A1A 9900 3A N 2 Gas TK-22 SBO Storage 84 83 HX-13 MX-3MX-4 Water Softener Interesterification PFD

11. E Algae Oil Storage Refined SBO Storage Lecithin Storage Beta Carotene Storage Diacetyl Storage Sorbic Acid Storage Mono- di glycerides Storage Ergo- califerol Storage Water Storage Oil Pasteurizatio n CIP Warm Water Aqueou s Phase Prep. 10 TK-01 TK-02 TK-03 TK-04 CIP TK-05 TK-06 TK-07 TK-08 TK-11 TK-12 TK-13 Oil Phase Prep. Tank 1 CIP P-01 P-02 P-03 P-04 Warm Water Oil Oil Phase Prep. Tank 1 CIP 9 12 8 7 6 5 34 12 13 Sea Salt Storage TK-10 P-05 14 15 TK-09 16 11 17 CIP 19 18 16 19 18 20 Margarine PFD Warm Water

12. High Pressure Pump P-06 Ammonia Compressor Pin Rotor Machine Scraped Surface Heat Exchanger Packaging P-07 CIP 20 22 23 Plate HX 01 Pasteurization 24 31 25 26 CIP 27 30 Margarine PFD 21

13. Overall Mass Balance for Oil Algae Oil Produced In Fermentation –127,606 kg / year Refined SBO Produced –3,424,720 kg / year Margarine Produced –117,520 kg / year Algae Oil –3,424,720 kg / year refined SBO

14. Energy Sinks/Loads and Streams Steam Boiler Q = 2,035,547 BTU / hr Fermentor Heat Exchanger Silicone Antifoam Direct Steam Rejector Paratherm Boiler Q = 895024.3 BTU / hr Water Cooler 48.9 °C – 29.4 °C Q = 482,348.6 BTU / hr Emulsification 25 °C – 35.78 °C Q = 28,600 BTU / hr Pasteurization 35.78 °C – 43.3 °C Q = 9,500 BTU / hr Crystallization 43.3 °C – 10 °C Q = - 57,200 BTU/ hr

15. Equipment Sizing For Fermentation Process EquipmentSize (gal) TK-111,000 TK-240,000 TK-31,200 TK-4300 TK-5300 MT-122,000 10L Seed Fermenter2.65 1000L Seed Fermenter265 10% Seed Fermenter3,500 Main Fermenter35,000 CO 2 Lysing Tank12,000 DAF Tank24,000

16. Equipment for SBO Refining Component Sizing TK-0120000 gal TK-0225 gal TK-03400 gal TK-04600 gal TK-0515 gal TK-061500 gal TK-071500 gal TK-084000 gal TK-0935 gal TK-10250 gal TK-11600 gal Component Sizing TK-12100 gal TK-131500 gal TK-144000 gal TK-1510000 gal TK-161000 gal TK-17250 gal TK-181200 gal TK-19400 gal TK-207500 gal TK-21400 gal TK-225000 gal MX-1100 gal MX-2100 gal MX-3500 gal MX-4500 gal Component Sizing HX-1202490.49 BTU/hr HX-212603.83 BTU/hr HX-312603.83 BTU/hr HX-415340.19 BTU/hr HX-519900.73 BTU/hr HX-636567.59 BTU/hr HX-7109454.00 BTU/hr HX-839541.70 BTU/hr HX-997016.05 BTU/hr HX-1049571.68 BTU/hr HX-11159620.87 BTU/hr HX-1292228.34 BTU/hr HX-1346342.34 BTU/hr TanksHeat Exchangers

17. Margarine Equipment Sizing ComponentSize P-010.25 GPM P-023.5 GPM P-031 GPM P-041 GPM P-053.5 GPM P-06110 GPM P-074.25 GPM Emulsion Tank 12000 L Emulsion Tank 22000 L High Pressure Pump2475 L / hr Plate Heat Exchanger6 ft^2 Scraped Surface Heat Exchanger2150 L / hr Pin Rotor Machine2000 L/hr Tub Filling Machine2740 L/hr Ammonia Compressor18 ton ComponentSize TK-011000 L TK-025000 L TK-0320 L TK-042 L TK-052 L TK-065 L TK-0710 L TK-082 L TK-09500 L TK-101000 L TK-11500 L TK-121500 L TK-131500 L

18. Costing Capitol Costs -Refining $ 11.54 Million - Algae Oil Production $ 20.25 Million - Margarine Production $ 1.8 Million - Chemicals and Ingredients $.22 Million Operating Costs - Refining $ 2.64 Million - Algae Oil Production $ 6.10 Million - Margarine Process $ 0.24 Million - Chemicals and Ingredients $ 4.7 Million Capital Costs -Refining $ 11.54 Million - Algae Oil Production $ 20.25 Million - Margarine Production $ 1.8 Million - Chemicals and Ingredients $.22 Million

19. ChemicalUnit PriceAmt. RequiredYearly Price Total Crude SBO$867.40/ton3,196.9423 tons$2,773,027.75 SBO Hardfat$0.46/lb1,544,895.744 lb$710,652,04 50% Citric Acid$8.51/gal9,118.579 gal$77,599.11 Acid Activated Clay$209/ton15.887 tons$3,320.38 99% Sodium Methoxide$14/kg6,863.348 kg$96,086.87 Nitrogen$7.7825/m³13.114 m³$102.06 Paratherm NF *$21.21/gal10,000 gal$212100.00 4% Sodium Hydroxide$4.39/L484.737 L$2,128.00 Enzyme$576/L60.053 L$34,590.53 Sea Salt (Fermentation)$600/ton672.44 tons$403,464.00 Ethanol bottoms$140/ton199.46 tons$27,924.40 CO 2 Free1,096,000 kg- Lecithin$0.81/lb19,534.7 lb$15,823.10 Sea Salt (Margarine Process)$600/ton739.449 tons$443,669.40 Ergocaliferol$18234/kg0.36628 lb$3,029.43 Mono/Di Glycerides$0.1/kg48,836.8 lb$10,780.75 Beta-Carotene$1250/kg193.8 lb$109,883.00 Diacetyl$2.5/kg24.42 lb$27.69 Ammonia *$500/ton18 tons$9,000.00 Sorbic Acid$8250/ton9,767.36$40,290.40 * Only capitol costs Chemical Costs

20. Questions ?

21. TK-3 Acidic Solution TK-4 Alkaline Solution TK-5 Reclaim Water TK-6 Fresh Water 11 12 13 10 21 2223 24 20 TK-1 Alkaline Storage TK-2 Acidic Storage 2 1 CIP Returning Liquid 3 4 5 Water 8 9 6 14 15 16 Steam HX-1 17 CIP Supplying Liquid Drain 7 19 18 Filter CIP System

22. Drying Process 333435 Biomass + Water InBiomassWater Out 22,902.52 6,168.82 16,733.71 Liquid 19,146 3,829 15,317 2,011 1,990.42 20.11 1,746 349 1,397 3637 Dry BiomassEvaporated Water 2,339.66 3,829.16 SolidLiquid Centrifuge 2Dryer