2. Freshwater metagenomics NO 3 - N2N2 NH 4 + O2O2 H2H2 Me ox Me red C2H3O2-C2H3O2- CO 2 Acyl-HSL HPO 4 2- C 6 H 12 O 6 SO 4 2- HS - Me 2+ MeS

3. Freshwater metagenomics NO 3 - N2N2 NH 4 + O2O2 H2H2 Me ox Me red C2H3O2-C2H3O2- CO 2 Acyl-HSL HPO 4 2- C 6 H 12 O 6 SO 4 2- HS - Me 2+ MeS

4. Trout Bog 0.01 km 2, z max = 8 m Sparkling Lake 0.6 km 2, z max = 20 m Lake Mendota 40 km 2, z max = 24 m

5. Erken Mälaren Metagenomic sequencing of Freshwater Microflora in Swedish Lakes Vättern Mälaren: Eutrophic, 1140 km 2, third largest lake in Sweden, max depth 64m Erken: Mesotrophic, 24 km 2, GLEON site Vättern: Oligotrophic, 1912 km 2, deep (128 m) small drainage area.

6. Collaborators Ramunas Stepanauskas – Bigelow Marine Laboratory, Maine Trina McMahon – UW-Madison, WI Stefan Bertilsson – Uppsala University, Sweden

7. Other random stuff…

8. MERIS satellite for remote sensing Colleen Mouw, Space Science and Engineering Center, UW

9. Actual data….