1. AP Biology 2005-2006 Chapter 5. Macromolecules

2. AP Biology 2005-2006 Macromolecules  Smaller organic molecules join together to form larger molecules  macromolecules  4 major classes of macromolecules:  carbohydrates  lipids  proteins  nucleic acids

3. AP Biology 2005-2006 Polymers  Long molecules built by linking chain of repeating smaller units  polymers  monomers = repeated small units  covalent bonds

4. AP Biology 2005-2006 How to build a polymer  Condensation reaction  dehydration synthesis  joins monomers by “taking” H 2 O out  1 monomer provides OH  the other monomer provides H  together these form H 2 O  requires energy & enzymes

5. AP Biology 2005-2006 How to break down a polymer  Hydrolysis  use H 2 O to break apart monomers  reverse of condensation reaction  H 2 O is split into H and OH  H & OH group attach where the covalent bond used to be  ex: digestion is hydrolysis

6. AP Biology 2005-2006 Carbohydrates

7. AP Biology 2005-2006 Carbohydrates  Carbohydrates are composed of C, H, O carbo - hydr - ate CH 2 O (CH 2 O) x C 6 H 12 O 6  Function:  energy u energy storage  raw materials u structural materials  Monomer: sugars  ex: sugars & starches

8. AP Biology 2005-2006 Sugars  Most names for sugars end in -ose  Classified by number of carbons  6C = hexose (glucose)  5C = pentose (fructose, ribose)  3C = triose (glyceraldehyde)

9. AP Biology 2005-2006 Simple & complex sugars  Monosaccharides  simple 1 monomer sugars  glucose  Disaccharides  2 monomers  sucrose  Polysaccharides  large polymers  starch

10. AP Biology 2005-2006 Building sugars  Dehydration synthesis | glucose | glucose glycosidic linkage monosaccharidesdisaccharide | maltose

11. AP Biology 2005-2006 Polysaccharides  Polymers of sugars  costs little energy to build  easily reversible = release energy  Function:  energy storage  starch (plants)  glycogen (animals)  building materials = structure  cellulose (plants)  chitin (arthropods & fungi)

12. AP Biology 2005-2006 Polysaccharide diversity  Molecular structure determines function  isomers of glucose  How does structure influence function…

13. AP Biology 2005-2006 Digesting starch vs. cellulose

14. AP Biology 2005-2006 Cow can digest cellulose well; no need to eat supplemental sugars Gorilla can’t digest cellulose well; must supplement with sugar source, like fruit

15. AP Biology 2005-2006 Glycemic index  Which food will get into your blood more quickly?  apple  rice cakes  corn flakes  bagel  peanut M&M

16. AP Biology 2005-2006 Glycemic index  Which food will get into your blood more quickly?  apple36  rice cakes82  corn flakes84  bagel72  peanut M&M33

17. AP Biology 2005-2006 Lipids

18. AP Biology 2005-2006 Lipids  Lipids are composed of C, H, O  long hydrocarbon chain  Diverse group  fats  phospholipids  steroids  Do not form polymers  big molecules made of subunit smaller molecules  not a continuing chain

19. AP Biology 2005-2006 Fats  Structure:  glycerol (3C alcohol) + fatty acid dehydration synthesis  fatty acid = long HC “tail” with COOH group at “head”

20. AP Biology 2005-2006 Fat  Triacylglycerol  3 fatty acids linked to glycerol  ester linkage = between OH & COOH

21. AP Biology 2005-2006 Dehydration synthesis

22. AP Biology 2005-2006 Fats  Long HC chain  polar or non-polar?  hydrophilic or hydrophobic?  Function:  energy storage  very rich  2x carbohydrates  cushion organs  insulates body  think whale blubber! “Let’s go to the video tape!” (play movie here)play movie here

23. AP Biology 2005-2006 Saturated fats  All C bonded to H  No C=C double bonds  long, straight chain  most animal fats  solid at room temp.  contributes to cardiovascular disease (atherosclerosis) = plaque deposits

24. AP Biology 2005-2006 Unsaturated fats  C=C double bonds in the fatty acids  plant & fish fats  vegetable oils  liquid at room temperature  the kinks made by double bonded C prevent the molecules from packing tightly together

25. AP Biology 2005-2006 Cholesterol  Important cell component  animal cell membranes  precursor of all other steroids  including vertebrate sex hormones  high levels in blood may contribute to cardiovascular disease

26. AP Biology 2005-2006 Proteins

27. AP Biology 2005-2006 Proteins  Most structurally & functionally diverse group of biomolecules  Function:  involved in almost everything  enzymes  structure (keratin, collagen)  carriers & transport (membrane channels)  receptors & binding (defense)  contraction (actin & myosin)  signaling (hormones)  storage (bean seed proteins)

28. AP Biology 2005-2006 Proteins  Structure:  monomer = amino acids  20 different amino acids  polymer = polypeptide  protein can be 1 or more polypeptide chains folded & bonded together  large & complex molecules  complex 3-D shape

29. AP Biology 2005-2006 Amino acids  Structure:  central carbon  amino group  carboxyl group (acid)  R group (side chain)  variable group  confers unique chemical properties of the amino acid —N——N— H H H | —C— | C—OH || O R

30. AP Biology 2005-2006 Nonpolar amino acids  nonpolar & hydrophobic Why are these nonpolar & hydrophobic?

31. AP Biology 2005-2006 Polar amino acids  polar or charged & hydrophilic Why are these polar & hydrophillic?

32. AP Biology 2005-2006 Building proteins  Peptide bonds: dehydration synthesis  linking NH 2 of 1 amino acid to COOH of another  C–N bond peptide bond

33. AP Biology 2005-2006 Building proteins  Polypeptide chains  N-terminal = NH 2 end  C-terminal = COOH end  repeated sequence (N-C-C) is the polypeptide backbone  grow in one direction

34. AP Biology 2005-2006 Protein structure & function hemoglobin  function depends on structure  3-D structure  twisted, folded, coiled into unique shape collagen pepsin

35. AP Biology 2005-2006 Protein structure & function  function depends on structure  all starts with the order of amino acids  what determines that order of amino acids? “Let’s go to the video tape!” (play movie here) lysozyme: enzyme in tears & mucus that kills bacteria the 10 glycolytic enzymes used to breakdown glucose to make ATP

36. AP Biology 2005-2006 Primary (1°) structure  Order of amino acids in chain  amino acid sequence determined by DNA  slight change in amino acid sequence can affect protein’s structure & it’s function  even just one amino acid change can make all the difference!

37. AP Biology 2005-2006 Sickle cell anemia “Let’s go to the video tape!” (play movie here)

38. AP Biology 2005-2006 Denature a protein  Disrupt 3° structure  pH  salt  temperature  unravel or denature protein  disrupts H bonds, ionic bonds & disulfide bridges  Some proteins can return to their functional shape after denaturation, many cannot

39. AP Biology 2005-2006 Any Questions??