1. TisXell Regeneration System

2. Company Background …(1) QuinXell Technologies Pte Ltd was founded in 2011 QuinXell is the latest addition to Quintech Life Sciences (QLS) family of companies established to tap into the emerging Life Science industry QuinXell will focus in the area of Regenerative Medicine through its innovative cell culture-based technologies

3. Company Background …(2) Research on QuinXell’s TisXell Regeneration System was initiated as a joint project between the National University of Singapore (NUS) and Singapore Polytechnic (SP), with the National University Hospital (NUH) providing samples for applications Scientific papers supporting the benefits of biaxial bioreactor on functional tissue growth (tissue engineered constructs or TECs) on 3D scaffolds have been published in international journals for more than 10 years

4. Background Information …(1) Regenerative Medicine has been hailed as a revolutionary medical advancement, breathing new life into patient care Transplantation through in vitro cultures of organs and tissues or Tissue Engineering (TE) is a standardized and reproducible method to create functional tissues in the laboratory These tissues are utilized to replace, repair or regenerate organs loss due to disease, trauma, failure or cogenital defects

5. Background Information …(2) Traditionally, spinner flasks, rotating wall vessels (RWV), perfusion and uniaxial bioreactors are employed for TE QuinXell’s unique TisXell Regeneration System offers an optimal condition for high growth and high proliferation of cell cultures compared to conventional methods. TisXell controlled environment provides optimal nutrients and gases to growing cells and triggers cellular mechano- transduction signalling pathways to stimulate tissue remodelling onto a 3D scaffold.

6. Application Fields For research institutes, hospitals, biotechnology, pharma in Regenerative medicine Tissue engineering Translational medicine Tissue and organ transplant

7. Cell Types Primary Cells Stem Cells Bone Cells Muscles Cells And many more…

8. Conventional Methods Types of BioreactorsAdvantagesDisadvantages Spinner flasks Improved fluid flow (as compared to static vessels) Turbulence generated can be detrimental for seeded cells and newly laid down ECM Perfusion bioreactors Enhances mesenchymal stem cell proliferation and osteogenic differentiation in scaffold constructs Non-homgenous cellular distribution Cells at frontal zones are washed away by the oncoming perfusion flow with higher flow rates Rotating wall vessel (RWV) bioreactors Low sheer forces and 3D high mass transfer (similar to perfusion bioreactors) Non-homogenous cellular distribution and ECM deposition In the free floating culture, collision between scaffolds and bioreactor walls may induce cellular damage and disrupt cellular attachment and matrix deposition on the scaffolds

9. TisXell Novel Approach Types of BioreactorsAdvantages Biaxial revolving bioreactor e.g. TisXell Integrated perfusion system allows for exchange between the vessel and reservoir with maximal mass transfer under low sheer force Biaxial revolution of the culture vessel led to more homogenous cellular and ECM deposition – at the surface and the deeper core of the scaffolds Cellular scaffolds were secured by pins to avoid scaffold and vessel wall collisions

13. TisXell Regeneration System

14. TisXell – Unique Selling Points …(1)  Patented biaxial revolution in two independent orthogonal axes (spin and tumble simultaneously)  Flexible operational modes available – biaxial, uniaxial, swing modes

15. TisXell – Unique Selling Points …(2)  Efficient fluid transport within the 3D scaffolds, allowing optimal nutrients and waste exchange to and from the cells, penetrating the deepest core of the scaffolds  Spherical design of vessel reduces drag and promotes uniform fluid mixing

16. TisXell – Key Benefits …(1)  Accelerates cell growth, differentiation and cell proliferation, mimicking native extracellular matrix (ECM)  Supports homogenous cell culture at the surface and core of the 3D scaffolds  Maintains functionality and viability of tissue constructs

17. TisXell – Key Benefits …(2)  Reduces formation of necrotic neotissue that interferes with subsequent implantation process  Promotes integration of implants with surrounding tissue and support the structural integrity in regeneration of tissues and/or organs

20. Key Benefits for Bone Applications …(1) In Vitro  Homogenous cellular distribution  Rapid stem cell expansion – up to 2-fold (or more) improvement in mass transfer in thicker grafts  High viability  Robust osteogenic differentiation and mineralization

21. Key Benefits for Bone Applications …(2) In Vivo  Increased bone formation after transplantation  Promotes new vascularization

42. TisXell System Configuration I TisXell Regeneration System, Basic Includes o Two spherical glass vessels of 500ml and 1000ml, 1 each o Revolving stand to hold one vessel, 1 o Integrated peristaltic pump, 1 o Medium reservoir, 1 o Remote touch screen panel, 1 o Tubings and connectors starter kit, 1 o Scaffolds starter kit, 1

43. TisXell System Configuration II TisXell Regeneration System, Complete Includes o Two spherical glass vessels of 500ml and 1000ml, 1 each o Revolving stand to hold one vessel, 1 o Integrated peristaltic pump, 1 o Medium reservoir, 1 o Remote touch screen panel, 1 o Tubings and connectors starter kit, 1 o Scaffolds starter kit, 1 o Set of probes, 1 o Applikon console and monitor, 1