1. The Biotechnology Center of Excellence Bringing Biotech from the Bench to the K-12 Classroom Event: Regenerative Medicine Webinar Date: May 22, 2014, 4pm & 7pm CST Location: Lindblom Math & Science Academy

3. Hortense Brice, Presenter hebrice@gmail.com Dr. Emily Ferrin, Facilitator emily.ferrin@northwestern.edu BCoE.net osep.northwestern.edu

4. Webinar Objectives  As a Result of Webinar Participation, You Will: 1.Realize the Need for Regenerative Medicine Practices and Procedures 2.Identify the Tools and Technologies of Regenerative Medicine 3.Understand the Challenges, Promise and Potential of Regenerative Medicine 4.Identify the Potential Benefits of Regenerative Medicine to America’s Costly Health Care System

5. “Body Builders”: The Emerging Science of Regenerative Medicine

6. The Beginning……… Charles Vacanti & Robert Langer (1997)

7. Characterizing Regenerative Medicine 1.Regenerative medicine is a broad definition for innovative medical therapies that will enable the body to repair, replace, restore and regenerate damaged or diseased cells, tissues and organs. (Mayo Clinic) 2.Science Merges with Technology to Achieve Self-Healing Regenerative medicine uses procedures to repair or replace damaged or diseased tissues and organs—Many traditional therapies only treat symptoms. 3.Tools and Procedures (Biofabrication or Additive Manufacturing) of Regenerative Medicine Tissue Engineering: Tissue Repair/Replacement and Lab Grown Organs Technologies Stem cells Natural and Synthetic Scaffolds 3-D Printing and Chip Technologies 4. Highly Interdisciplinary Field where Biology and Chemistry meet: Engineering Material Science

8. Areas of Regenerative Medicine 1.Artificial Organs: Medical Devices ( Lab Grown Bladder ) 2.Tissue Engineering & Biomaterials Scaffolds

9. Areas of Regenerative Medicine 3. Cellular Therapies Use of Stem Cells (From Patient) Development of Regenerative Medicine Treatments. Enhance Regeneration of Tissues and Organs. 4.Clinical Trials Many Currently in Progress. NIH and Private Organizations.

10. Regenerative Medicine Procedures/Protocols Engineer Scaffolds Hydrogel Spaghetti Highways Ear Scaffold

11. Regenerative Medicine Procedures/Protocol Seed Scaffold With Stem Cells

12. Regenerative Medicine Procedures/Protocol Incubate Seeded Scaffold in Bioreactor Transplant into An Animal Model

13. Regenerative Medicine And PRP Therapy PRP: Platelet Rich Plasma Therapy Concentrate Platelets from Patients Blood Sample Platelets Contain Cytokines and Growth Factors PRP Accelerates Healing and Reduces Pain Bill Nye “The Science Guy” and PRP Therapy

14. Regenerative Medicine Pioneer Wake Forest Institute for Regenerative Medicine ( WFIRM ) Dr. Anthony Atala First to Engineer/Transplant Lab-Grown Organ into a Human Transplant was Successful Currently Developing Organ on a Chip Program

15. Regenerative Medicine Pioneer Dr. Paolo Macchiarini ( Karolinska Institute) 2008: Implanted World’s First Donor Trachea Recipient: Claudio Castillo Survived Procedure—Now Has Normal Respiratory Function

16. Regenerative Medicine Pioneer McGowan Institute of Regenerative Medicine (University of Pittsburg) Dr. Stephen Badylak Removed Cells from Pig Bladder Extra Cellular Matrix (ECM) Dried: Forms “Pixie-Dust” Re-grows Severed Digits and New Muscle Tissue

17. Regenerative Medicine Pioneer AFIRM: Armed Forces Institute Of Regenerative Medicine. Treatment Procedures for Severely Wounded Soldiers Funded and Managed by US Army Partnership with Wake Forest Institute for Regenerative Medicine

18. Regenerative Medicine Innovator Dr. Geraldine Hamilton (Wyss Institute) Organ on a Chip Technology (Drug Testing Tool)

19. Regenerative Medicine Innovator Jordan Miller ( Rice University) Uses 3-D Print Technology Engineers Blood Vessels Using Sugar

20. Regenerative Medicine Innovator Ramille N. Shah, PhD ( Northwestern University) Leader in Field of 3D-Printable Materials Engineers new 3D-Inks Creates Porous Scaffolds Technique: Additive Manufacturing (Nanofiber Scaffold For Cartilage Regeneration)

21. Regenerative Medicine Challenges 1.Design of Biomaterials that Function in the Body 2.Getting Enough Cells and Cell Types for Engineering Tissues and Organs 3.Vascularity: Engineering Blood Vessels that Supply Nutrients, Oxygen and Signals to Bioengineered Tissues and Organs 4.Cost of Tissue and Organ Development Procedures

22. Organ Decellularization Approach Dr. Harald Ott http://www.youtube.com/watch?v=5wfdhB_V yJw

23. Challenge To Regenerative Medicine Bioengineering of Organs

24. Potential of Regenerative Medicine Chip Technology Reduces Need for Animal Testing 3-D Printed Organs on Chips Used to Test Vaccines

25. 3-D Elastic Membrane Fits Heart’s Epicardium 3-D Printer Creates Heart Membrane Promise of Regenerative Medicine

26. The Business of Regenerative Medicine Organovo  3-D Bioprinting Company Started A Collaboration with NIH (January 2014) Goal: Bioprinting of 3D Living Tissues Eliminate Challenges to New Therapy Development Animal Models: Poor Predictors of Drug Efficacy and Toxicity

27. The Business of Regenerative Medicine Tengion  A Biotechnology Company  Company Platform for Engineering Tissues/Organs  Organ Regeneration Process o Doctors send Tissue Sample from Diseased or Failing Organ to Tengion o Tengion Selects and Multiplies Healthy Cells o Place Cells on an Organ-Shaped Scaffold o Result: A Neo-Organ for Transplant

28. Futuristic! Stem Cells + Organ Scaffold + 3D Printer = Libraries of Replacement Organs?

29. Regenerative Medicine Summary Regenerative Medicine Approaches o Use of Patients Stem Cells o 3-D Bioprinting of Tissues and Organs o Organ Decellularization o PRP: Plasma Rich Platelet Therapy Human Treatment Veterinary Medicine Therapy

30. Regenerative Medicine And Healthcare New Era in Medicine: Move from Treatment to Cures (Self-Healing) Eliminate Organ Transplant Shortage Reduce Health Care Costs Now A Medical and Commercial Reality Over 60, 000 Stem Cell Transplants Worldwide 2012: Cell Therapies Generated More Than $900 Million

31. Thank you! osep.northwestern.edu emily.ferrin@northwestern.eduhebrice@gmail.com