1. Introductory Chemistry: Concepts & Connections Introductory Chemistry: Concepts & Connections 4 th Edition by Charles H. CorwinBiochemistry Christopher G. Hamaker, Illinois State University, Normal IL © 2005, Prentice Hall Chapter 20

2. 2 Introduction to Biochemistry There are 30 elements which are essential for life. The study of the chemistry of living things is biochemistry. Biological compounds are often large and complex with molar masses greater than 1,000,000 g/mol. These large molecules are polymers of smaller molecules.

3. Chapter 203 Biological Compounds

4. Chapter 204 Proteins Proteins are naturally occurring polymers composed of many amino acids. An amino acid has both an amine and a carboxylic acid functional group. Amino acids are linked together by amide bonds which are referred to as peptide linkages.

5. Chapter 205 Carbohydrates A carbohydrate is either a simple sugar or a polymer composed of many simple sugars. A carbohydrate usually contains either an aldehyde or ketone functional group and several alcohol groups. Starch is a carbohydrate polymer composed of glucose units linked together by glycoside linkages.

6. Chapter 206 Lipids A lipid is a water-insoluble compound usually composed of an alcohol and one or more carboxylic acid molecules. Fats and oils are esters of glycerol, an alcohol that has three –OH groups. As a result, each molecule of a fat or an oil contains three ester groups from three carboxylic acid molecules joined to one glycerol molecule.

7. Chapter 207 Nucleic Acids A nucleic acid is a biochemical polymer composed of a very large number of individual units. Each unit in the nucleic acid contains a sugar molecule attached to an organic nitrogen-containing molecule and an attached phosphate group. The units are attached together by phosphate linkages.

8. Chapter 208 Amino Acids Proteins are composed of amino acids. An amino acid has an amine (-NH 2 ) and a carboxy group (-COOH) attached to a carbon atom with side chain (R) attached to the  -carbon. There are twenty naturally occurring amino acids in human proteins. Each one has a different side chain.

9. 9 The 20 Natural Amino Acids Acidic Amino Acids Basic Amino Acids Neutral Amino Acids

10. Chapter 2010 Polypeptides In proteins, amino acids are linked together with peptide linkages or a peptide bond. In a dipeptide, two amino acid units are joined together by a peptide bond. In a polypeptide, up to 50 amino acids are linked together. Human insulin is composed of 48 amino acids.

11. Chapter 2011 Primary Structure of Proteins Proteins are polypeptides composed of hundreds or thousands of amino acid units. The primary structure of a protein is the sequence of amino acids. Individual amino acids are represented by the colored ovals in the figure below. The replacement of one amino acid by another in the primary structure of a protein can completely alter its biological activity.

12. Chapter 2012 Secondary Structure of Proteins When the peptide chain twists and bends, proteins acquire a secondary structure. There are two primary types of secondary structures,  -helix and pleated sheet. An  -helix is analogous to that in a coiled telephone cord.

13. Chapter 2013 Pleated Sheet In a pleated sheet, the chains run antiparallel to each other. It looks like a sheet of paper folded in an accordion shape. Secondary structure in proteins is a result of hydrogen bonds.

14. Chapter 2014 Tertiary Structure of Proteins The overall three-dimensional structure of a protein is referred to as its tertiary structure. The tertiary structure of a protein may be long and extended or compact and folded. The tertiary structure of a protein is held together by intermolecular forces.

15. Chapter 2015 Enzymes An enzyme is a protein that acts as a biological catalyst. Enzymes are incredibly selective for specific molecules. An enzyme can speed up a biochemical reaction so that the rate is a million times faster than it would be in the absence of the enzyme. Many reactions catalyzed by enzymes would be too slow without the enzyme to sustain life.

16. Chapter 2016 Enzyme Mechanisms The location where the reaction occurs on the enzyme is the active site. The molecule that reacts is the substrate. We can use the lock-and-key model to describe enzyme mechanisms. In the model, the key is the enzyme and the lock it the substrate.

17. Chapter 2017 Enzyme Reactions An enzyme reaction takes place in two steps. First, the substrate (S) binds to the active site on the enzyme (E). Step 1: E + S → ES Second, the enzyme releases two or more products (P 1 and P 2 ). Step 2: ES → E + P 1 + P 2

18. Chapter 2018 Enzyme Reactions The active site of an enzyme has a shape designed to specifically bind its substrate. Once the reaction has occurred, the active site is free to bind another substrate molecule and repeat the reaction.

19. Chapter 2019 The Enzyme Ptyalin The enzyme ptyalin is in human saliva and catalyzes the breaking down of starch molecules into sugar units. The reaction would take about a two years to occur without the enzyme.

20. Chapter 2020 Carbohydrates The word carbohydrate means “hydrates of carbon” and many have the empirical formula CH 2 O. Carbohydrate names usually end in the suffix -ose. Carbohydrates have an aldehyde or ketone functional group and several hydroxyl groups.

21. Chapter 2021 Simple Sugars A simple sugar molecule typically has three to six carbons, and aldehyde or ketone group, and a few hydroxyl groups. A simple sugar is referred to as a monosaccharide. A monosaccharide with an aldehyde group is an aldose and one with a ketone group is a ketose.

22. Chapter 2022 Aqueous Structures of Sugars In aqueous solution, sugar molecules usually exist in ring structures. Glucose forms a cyclic structure by joining a hydroxyl group to an aldehyde group. The resulting ring structure has 5 carbon atoms and 1 oxygen atom.

23. Chapter 2023 Disaccharides A disaccharide is two monosaccharide molecules joined together. In the formation of a disaccharide, two simple sugars split out water and are joined together by a special –O– bond called a glycoside linkage.

24. Chapter 2024 Polysaccharides A polysaccharide is a polymer of many monosaccharide linkages joined by glycoside linkages. Starch and cellulose are both polysaccharides composed only of glucose units but with slightly different 3-dimentional structures. Starch Cellulose

25. Chapter 2025 Lipids Unlike most other biological compounds, lipids are not water soluble. Lipids include –Fats and oils –Waxes –Steroids –The water insoluble vitamins A, D, E, and K

26. Chapter 2026 Fatty Acids A fatty acid is a carboxylic acid with a long hydrocarbon chain. Fatty acids can be saturated or unsaturated.

27. Chapter 2027 Triglycerides A triglyceride is a lipid formed from glycerol, a trihydroxy alcohol, and three fatty acids. When three fatty acids react with glycerol, a triglyceride is formed with three ether linkages.

28. Chapter 2028 Fats In triglycerides obtained from animals, the fatty acids are mostly saturated and have few double bonds. A semisolid lipid obtained from an animal source is a fat. The following structure is and example of a saturated triglyceride from animal fat.

29. Chapter 2029 Oils A liquid lipid obtained from a plant source is an oil. Triglycerides obtained from plants have mostly unsaturated fatty acid chains. The following structure is and example of an unsaturated triglyceride from a vegetable oil.

30. Chapter 2030 Nucleic Acids Nucleic acids are biochemical compounds found in every living cell. Nucleic acids contain the genetic information responsible for the reproduction of a species. There are two basic types of nucleic acids: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). A nucleic acid is a polymer composed of many repeating units, each of which is called a nucleotide.

31. Chapter 2031 Nucleotides Each nucleotide consists of three similar component molecules: a five-carbon sugar, a nitrogen-containing base, and a molecule of phosphoric acid. The five-carbon sugars are similar in RNA and DNA. The two sugars are identical except for a hydroxyl group. The structures are shown here:

32. Chapter 2032 Nitrogen Bases There are five nitrogen bases. Three are found in both DNA and RNA, one is found only in DNA and one is found only in RNA. Their structures are shown below:

33. Chapter 2033 Nucleotide Structures Nucleotides for DNA and RNA are the sugar with a phosphoric acid and the nitrogen base attached. Shown here are structures of a DNA and RNA nucleotide.

34. Chapter 2034 DNA and RNA DNA and RNA are polymers of individual nucleotides. A segment is shown here. A DNA molecule is actually two strands of DNA wound together in a spiral structure called a double helix. The double strand is held together by hydrogen bonds between nitrogen pairs.

35. Chapter 2035 DNA Double Helix An adenine (A) on one strand always hydrogen bonds to a thymine (T) on the other strand. Also, a cytosine (C) on one strand always hydrogen bonds to a guanine (G) on the other strand. These interactions give DNA its characteristic structure.

36. Chapter 2036 Conclusions There are 4 major types of biological molecules: –Proteins are composed of amino acids. –Carbohydrates are composed of simple sugar molecules. –A lipid is a water insoluble biological molecule. –A nucleic acid is a polymer composed of a sugar molecule, a nitrogen base, and phosphoric acid.

37. Chapter 2037 Conclusions Continued An enzyme is a catalyst for biological reactions. Enzymes work by a lock-and-key mechanism where only a specific substrate fits into the enzyme to react. Reactions catalyzed by enzymes can be completed in a matter of seconds where the reaction would normally take many years.

38. Chapter 2038 Conclusions Continued A triglyceride is an ester of glycerol and three different fatty acids. A fat is a triglyceride from an animal source. An oil is a triglyceride from a plant source. A nucleic acid is a molecule that carries genetic information. DNA and RNA are the two basic types of nucleic acids.