1. Experiment 4- Gene Expression Study in Arabidopsis Thaliana

2. RNA Blot Quantitative RT-PCR Microarrays Tools For Studying Differential Gene Activity

3. What Are The Major Differences Between DNA and RNA?

4. RNA Has a Uracil Instead of Thymine

5. RNA Has Ribose Sugar in Nucleotide

6. Eukaryotic RNAs Are Single-Stranded Polynucleotides Nucleotides Joined By Phosphodiester Bonds Like All Nucleic Acids Order 5’  3’ Leads to Function: Co-Linear With Gene Sequence

7. RNA Has Intra-Strand Double Helices or Secondary Structure

8. Total RNA Isolation Workflow Collecting plant materials (1-6) Lysis (7-11) (12-16) Washing (21-32) Precipitation & Binding (17-20) Elute RNA (33-40) Determining Concentration 1. RNA Isolation 2. Remove Genomic DNA RNase-Free DNase I 3. Electrophoresis Determining Quality of Isolated RNA

9. Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System

11. Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System- Part II Leaf Silique