Presentation is loading. Please wait.

Presentation is loading. Please wait.

Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J.

Published byEthan Strickland Modified over 8 years ago

Similar presentations

Presentation on theme: "Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J."— Presentation transcript:

1 Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J. Moss, Michaela de Clare, Pınar Pir, Jem Rowland, Wayne Aubrey, Ron Pateman, Mike Young, Mark Carrington, Ross D. King, and Stephen G. Oliver Open Biology Volume 3(2):120158 February 27, 2013 ©2013 by The Royal Society

2 Fluorescence labelling of yeast strains. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

3 Relative fluorescence measure detected using a BMG Optima plate reader at 580 nm (excitation)/612 nm (emission) (Cherry), 405 nm (excitation)/510 nm (emission) (Sapphire), 500 nm (excitation)/540 nm (emission) (Venus) and 440 nm (excitation)/490 nm (emissio... Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

4 Example of a high-throughput screening result. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

5 Network of connections between kinetoplastid targets and our hits. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

6 Antiplasmodial hits. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

Download ppt "Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J."

Similar presentations

Discovery Informatics Workshop February 2-3, 2012 NSF Workshop on Discovery Informatics Vasant Honavar Program Director Information & Intelligent Systems.

Discovery Informatics Workshop February 2-3, 2012 NSF Workshop on Discovery Informatics Vasant Honavar Program Director Information & Intelligent Systems.
Diel vertical migration of Arctic zooplankton during the polar night by Jørgen Berge, Finlo Cottier, Kim S Last, Øystein Varpe, Eva Leu, Janne Søreide,

Diel vertical migration of Arctic zooplankton during the polar night by Jørgen Berge, Finlo Cottier, Kim S Last, Øystein Varpe, Eva Leu, Janne Søreide,
TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes by Roshan L. Shrestha, Naoka Tamura, Anna Fries, Nicolas.

TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes by Roshan L. Shrestha, Naoka Tamura, Anna Fries, Nicolas.
Transmission and dose–response experiments for social animals: a reappraisal of the colonization biology of Campylobacter jejuni in chickens by Andrew.

Transmission and dose–response experiments for social animals: a reappraisal of the colonization biology of Campylobacter jejuni in chickens by Andrew.
1/03/09 De 89 à 98. 1/03/09 De 89 à 98 1/03/09 De 89 à 98.

1/03/09 De 89 à 98. 1/03/09 De 89 à 98 1/03/09 De 89 à 98.
Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila by Kyle A. McElroy, Hyuckjoon Kang,

Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila by Kyle A. McElroy, Hyuckjoon Kang,
Automating Biology using

Automating Biology using

Which muscles compromise human locomotor performance with age?

Which muscles compromise human locomotor performance with age?
Potency of irritation by benzylidenemalononitriles in humans correlates with TRPA1 ion channel activation by Christopher D. Lindsay, Christopher Green,

Potency of irritation by benzylidenemalononitriles in humans correlates with TRPA1 ion channel activation by Christopher D. Lindsay, Christopher Green,
High Throughput And High Content Screening. What Is HTS ? Biochemical target  Test many compounds  Compounds with desired effect on target = Hit  OR.

High Throughput And High Content Screening. What Is HTS ? Biochemical target  Test many compounds  Compounds with desired effect on target = Hit  OR.
Automating Drug Design Using Robot Scientists

Automating Drug Design Using Robot Scientists
Mineral–microbe interactions in deep-sea hydrothermal systems: a challenge for Raman spectroscopy by J. A. Breier, S. N. White, and C. R. German Philosophical.

Mineral–microbe interactions in deep-sea hydrothermal systems: a challenge for Raman spectroscopy by J. A. Breier, S. N. White, and C. R. German Philosophical.
Deleterious consequences of antioxidant supplementation on lifespan in a wild-derived mammal by Colin Selman, Jane S. McLaren, Andrew R. Collins, Garry.

Deleterious consequences of antioxidant supplementation on lifespan in a wild-derived mammal by Colin Selman, Jane S. McLaren, Andrew R. Collins, Garry.
Tuberculosis in Dr Granville's mummy: a molecular re- examination of the earliest known Egyptian mummy to be scientifically examined and given a medical.

Tuberculosis in Dr Granville's mummy: a molecular re- examination of the earliest known Egyptian mummy to be scientifically examined and given a medical.
Yeast Mnn9 is both a priming glycosyltransferase and an allosteric activator of mannan biosynthesis by Alexander Striebeck, David A. Robinson, Alexander.

Yeast Mnn9 is both a priming glycosyltransferase and an allosteric activator of mannan biosynthesis by Alexander Striebeck, David A. Robinson, Alexander.
Surpassing Mt. Everest: extreme flight performance of alpine bumble-bees by Michael E. Dillon, and Robert Dudley Biology Letters Volume 10(2):20130922.

Surpassing Mt. Everest: extreme flight performance of alpine bumble-bees by Michael E. Dillon, and Robert Dudley Biology Letters Volume 10(2):
Canadian fishery closures provide a large-scale test of the impact of gillnet bycatch on seabird populations by Paul Regular, William Montevecchi, April.

Canadian fishery closures provide a large-scale test of the impact of gillnet bycatch on seabird populations by Paul Regular, William Montevecchi, April.
Tumour–stromal interactions generate emergent persistence in collective cancer cell migration by William K. Chang, Carlos Carmona-Fontaine, and Joao B.

Tumour–stromal interactions generate emergent persistence in collective cancer cell migration by William K. Chang, Carlos Carmona-Fontaine, and Joao B.
The metabolic and mechanical costs of step time asymmetry in walking by Richard G. Ellis, Kevin C. Howard, and Rodger Kram Proceedings B Volume 280(1756):20122784.

The metabolic and mechanical costs of step time asymmetry in walking by Richard G. Ellis, Kevin C. Howard, and Rodger Kram Proceedings B Volume 280(1756):

Similar presentations

Ads by Google