1. BIOL 171 Lab
Fall 2014

2. Instructor:
Michelle Smith
Contact Information:

3. Class Web Sites: Syllabus Schedule Assignments Handouts
Syllabus
Schedule
Assignments
Handouts 3

4. Lab Time & Location Section 62109 Monday 2:30-5:15 Section 62356
Imiloa 106

5. Imiloa 136 Instructor’s Office Location & Hours
M 12:45-2:30, W 10:30-11:30
or by apt.

6. Biology and diversity of:
Course Topics
Biology and diversity of:
Measurements and statistics
Biological Molecules
Microscope use and cells
Enzyme kinetics
Meiosis and mitosis
Photosynthesis
Genetics
PCR
DNA electrophoresis
Bioinformatics
Prokaryotes

7. Cell Phones
Please turn it off before arriving to class!

8. Attendance Mandatory Be on Time! Don’t expect labs to end early.
Let instructor know if you anticipate an absence.

9. Lab Notebook 60 pts 4 note books lined turned in next lab
Figure 1. Frequency histogram of number of seeds per pod for koa haole, Leucaena leucocephala, seed pods.
Date: 9/30/14
Lab 04 Cells
Fig. 1. pseudostratified epithelia
pseudostratified epithelia
cillia
nuclei

10. 12 lab summaries (20 pts each) Late summaries deduct 3 pts
More than 1 week late not accepted
Lab Summary
Answer questions (typed)
Purpose, procedures, results, discussion
Be organized!
Microscope Use

11. Library Research Paper
20 pts
literature searches, scientific writing, citation procedures, and bibliography formatting
5 pages minimum
Late- 5 points deduction up to one week
WCC Library
citations
Fritts, T.H The brown tree snake, Boiga irregularis, a threat to Pacific islands. U.S. Fish Wildl. Serv., Biol. Rep. 88:23-25.

12. Pre-Lab Quizzes 13 pre-lab quizzes (15 points each) for 150 pts
laulima
Go to BIOL 171L site
May get questions from previous lab as well as current lab
10 best scores
No make-up quizzes
Don’t be late
Timed- 20 minutes

13. Lab Practicals: 2 exams 100 points each (200 pts) No make-up or retake
Form study groups

14. Grading Practical Examinations (2) 200 points
Pre-Lab Quizzes (13) 150 points
Laboratory Notebook (13) points
Laboratory Summaries (13) 240 points
Library Research Report points
TOTAL points
Grade cutoffs:
A = above 90%
B = 80%-89%
C = 70%-79%
D = 60%-69%
F = below 59%
No plus or minus final grades

15. Lab Photo Atlas
Adams, B. & Crawley, J. (2013). Van De Graff's Photographic Atlas for the Biology Laboratory (7th edition). Morton Pub. ISBN (~$40)

16. Cheating Plagiarism Academic Dishonesty
See student handbook on conduct code or

17. Lab Safety 01

18. Lab Policies
Sign Waiver

19. Preparation Read assignment before attending lab
Take pre-lab quiz up to hr before lab

20. You will be working with some toxic substances in lab
Lab Attire
You will be working with some toxic substances in lab
Lab coat
Gloves
Goggles
Proper clothing
No open-toed shoes

21. Lab Safety Prohibited Food Drink Smoking Cell phone Horseplay
Open-toed shoes

22. Lab Safety
Material Safety Data Sheet (MSDS) for each chemical used is available to you upon request.
Identify the location of the fire extinguisher, first aid kit, emergency shower, and eyewash

23. Handling Chemicals Treat all chemicals as though they were hazardous.
Do not mouth-pipette any chemical.
Do not ingest, inhale, touch any chemical.

24. Handling Organisms
All organisms, living or dead, should be treated with care and respect.
Some organisms may be hazardous if touched or ingested.
Avoid direct handling when possible.
Proper handling of microorganisms will be demonstrated.

25. Lab Safety Please Report The Following to the instructor: Injuries
Broken equipment
Broken glass or tools

26. Lab Safety
Broken glassware, slides and coverslips must be disposed of into the “GLASS ONLY” boxes
These guidelines will help to protect you from the harmful materials used in lab

27. Lab Safety Equipment
In case of an emergency, you should know the location and proper use of all the safety equipment provided in the laboratory.
Always immediately inform the instructor of any accident.

28. Fire Extinguishers (3) Squeeze handles. (2) Pull out locking key. (1)
Aim nozzle
at base of
fire.

29. Fire Extinguishers
Carbon dioxide fire extinguishers are suitable for many of the potential fire hazards in the general chemistry laboratories.
To operate this type of fire extinguisher, aim the nozzle at the base of the fire, pull out the locking key and depress the handles together.
This will release a discharge of carbon dioxide that will smother the fire.
Always inform the instructor of the use of a fire extinguisher so that it can be recharged.

30. Safety Eyewash
Any chemical contacting one’s eyes should be immediately and thoroughly rinsed out using the safety eyewash found in every laboratory.

31. Position head
between spigots.
Push handle to start
the flow of water!

32. Safety Eyewash Call for assistance to get to the eye wash station.
Position the head with eyes open between the spigots.
Push the handle to start the flow of water.
Flush for at least 15 minutes.

33. Safety Shower
Any chemical spill on a person should be washed off immediately and thoroughly using the safety shower.
Pull the chain to start
the flow of water!

34. Safety Shower Any chemical spill on a person
should be washed off immediately and
thoroughly using the safety shower.
Remove all contaminated clothing and stand under the shower.
Pull the chain to start the flow of water.
Washing should continue long enough to insure complete removal of the chemical, at least minutes.

35. Chemical Spills
Located on lab benches are containers of sodium bicarbonate to neutralize acid spills. These work by covering the spill of acid with a solid.
Contact your instructor immediately if there is a chemical spill.
Your instructor will handle any and all chemical spills.

36. Special Caution Handling Glassware
Protect hands.
Lubricate glass
surfaces.
NEVER force
the glassware!

37. Special Caution Handling Glassware
Many laboratory accidents involve cuts or puncture wounds due to improper manipulation of glassware.
Never force a piece of glassware when constructing experimental apparatus!
Use proper hand protection and lubricants such as glycerin or soapy water when possible.

38. Waste Disposal Discard waste as needed, during
and at the end of each laboratory period.

39. Waste Disposal
Specific guidelines need to be observed for waste disposal and will be given by your teacher.
Not Sure? PLEASE ASK!!!!
Waste Basket

40. Paper Products
Paper products that do not contain chemicals can be placed in the waste baskets.
Waste Basket

41. Solid and Liquid Chemicals
Solid and liquid chemicals need to be placed in appropriate containers.
The exact means will be given in the lab procedure or specified by the instructor.
Never dump chemicals down the drain! Never return unused chemicals to the original stock bottle!

42. Behavior Have a positive attitude!
Work cooperatively with group members

43. Clean-up Everyone participates Disinfect your work area
Put equipment away

44. Most Important Keep you eyes open to your surroundings.
If your not sure, ASK!