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July 14, 2015IM-SURE Symposium BioMiNT Alok Vij 1 Faculty Mentor: Professor AP Lee 2,3 Graduate Mentor: JS Fisher 2 Cell Encapsulation for Tissue Engineering.

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Presentation on theme: "July 14, 2015IM-SURE Symposium BioMiNT Alok Vij 1 Faculty Mentor: Professor AP Lee 2,3 Graduate Mentor: JS Fisher 2 Cell Encapsulation for Tissue Engineering."— Presentation transcript:

1 July 14, 2015IM-SURE Symposium BioMiNT Alok Vij 1 Faculty Mentor: Professor AP Lee 2,3 Graduate Mentor: JS Fisher 2 Cell Encapsulation for Tissue Engineering 1 Department of Materials Science and Engineering, Northwestern University 2 Department of Biomedical Engineering, University of California, Irvine 3 Department of Mechanical and Aeronautical Engineering, University of California, Irvine

2 July 14, 2015IM-SURE Symposium Agenda  Introduction  Materials  Methods  Results  Conclusions

3 July 14, 2015IM-SURE Symposium  Applications Tissue Engineering Scaffolds Immunoprotection of Implants Single-cell assays Cell Encapsulation Images: www.science.org, www.manager-magazin.de

4 July 14, 2015IM-SURE Symposium Agenda  Introduction  Materials  Methods  Results  Conclusions

5 July 14, 2015IM-SURE Symposium  PDMS devices  Alginate  Oleic acid  Calcium-containing polymerizing agent Materials Images: www-biol.paisley.ac.uk, www.babonline.org

6 July 14, 2015IM-SURE Symposium Materials  Alginate Biocompatible Non-degradable Gentle gelation Easily manipulated Images: www.lsbu.ac.uk

7 July 14, 2015IM-SURE Symposium Agenda  Introduction  Materials  Methods  Results  Conclusions

8 July 14, 2015IM-SURE Symposium Methods  Device Fabrication Photoresist Expose Develop PDMS cast Anneal Image modified from http://www.postech.ac.kr

9 July 14, 2015IM-SURE Symposium Methods  Alginate/cell suspension  Droplet generation  In-channel polymerization  Bead isolation  Bead harvesting  Cell culturing

10 July 14, 2015IM-SURE Symposium Agenda  Introduction  Materials  Methods  Results  Conclusions

11 July 14, 2015IM-SURE Symposium  Successes: Even distribution of cells Bead polymerization Droplet generation Bead isolation Bead harvesting  Work in progress: Microbead stability Cell viability Results Fibroblasts suspended in alginate

12 July 14, 2015IM-SURE Symposium Results  In-channel polymerization

13 July 14, 2015IM-SURE Symposium Results  Droplet Generation Before:

14 July 14, 2015IM-SURE Symposium Results  Droplet Generation Before:

15 July 14, 2015IM-SURE Symposium Results  Bead separation Before:

16 July 14, 2015IM-SURE Symposium Results  Bead Harvesting Before:

17 July 14, 2015IM-SURE Symposium Device Design  Before:

18 July 14, 2015IM-SURE Symposium Device Design  Before:

19 July 14, 2015IM-SURE Symposium Device Design  Before:

20 July 14, 2015IM-SURE Symposium Device Design  Before:

21 July 14, 2015IM-SURE Symposium Device Design  Before:

22 July 14, 2015IM-SURE Symposium Device Design

23 July 14, 2015IM-SURE Symposium Results  Droplet Generation After:

24 July 14, 2015IM-SURE Symposium Results  Bead Separation After:

25 July 14, 2015IM-SURE Symposium Results  Bead Harvesting After:

26 July 14, 2015IM-SURE Symposium Current Work  Bead stability

27 July 14, 2015IM-SURE Symposium Current work  Cell viability assessment

28 July 14, 2015IM-SURE Symposium Agenda  Introduction  Materials  Methods  Results  Conclusions

29 July 14, 2015IM-SURE Symposium Conclusions  Success!  Next steps: Integrate with other devices  Cell factor delivery  Concentration gradient generators  Cell sorters  Switches Publish

30 July 14, 2015IM-SURE Symposium Thank you!  Professor Abe Lee  Jeff Fisher  Said Shokair  National Science Foundation Jeff Fisher Abe Lee Said Shokair

31 July 14, 2015IM-SURE Symposium Questions?

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