1. Simulation of Created Design Documentation on the simulation process of a basic injector-separation channel model design

2. The Simulation Process In this simulation, a sample will be passed into the injector, and ‘Injected’ into the separation channel This is where the different species of the product will begin to separate from each other A signal of when each species passes a detector will be taken and plotted

3. Getting to the Simulation Window In order to simulate the design, the simulation window named “Cadence Analog Design Environment” needs to be opened To do this, go into the toolbar menu: Tool-> Analog Environment

4. Getting Variables The Variables that were used when adding the objects need to be added into the simulation variables This is done by selecting Variables -> Copy From Cellview from the toolbar, this will pick all the variables in the schematic

5. Editing Variables - I Now the values of those variables need to be changed To change them select Variables -> Edit from the toolbar, or click on the following side toolbar button

6. Editing Variables - II To change the values, type the value into the Value (Expr) field and click on Change Then select the Next variable from the table and continue Enter the following table of values, V1D and V3D need to be close to V4D value to prevent much of a voltage difference between the two V4L12.5 V4D3000 V3L0 V3D2900 V2L52.5 V2D0 V1L22 V1D2900 mu05000 0 mu14000 0 LOA D 0 D1400 D0400

7. Selecting Outputs In order to neatly see the results from the simulation, outputs are needed To select output, in the toolbar menu, go to: Outputs -> To Be Plotted -> Select On Schematic This will open up the schematic window again

8. Selecting the Pin In the Editor window, the values of wire that is selected will be plotted in a graph when the simulation is run So, select the ‘conc ’ output pin on the first detector and the ‘conc_out ’ output pin on the second detector

9. Output Plots When the conc pin is clicked a box pops up and when it is clicked again, conc and conc get highlighted, click OK, the two come under Outputs follow the same procedure for conc_out too

10. Choosing the Analysis To select the simulation type, click on the “Choose Analyses...” button on the side toolbar

11. The DC Analysis In the “Choosing Analyses” window, select dc, Enabled, and Save DC Operating Point and click OK

12. The First Simulation Once all the values have been inputted, click on the toolbar menu: Simulation->Netlist and Run on the toolbar menu or click the “Netlist and Run” button on the side toolbar

13. The Log File After this simulation has been run, a log file will appear This file is the results of the simulation and has some values that need to be inputted into the design

14. Edit the Properties Some properties of the injector need to be changed To do this go to: Edit -> Properties -> Objects… q

15. Editing Properties Input the values from the log file of the currents and concentrations into the corresponding properties of the injector, i.e., current 1 = cur_I1 etc One change that needs to be done to the values is that they should all be inputted as positive numbers

16. Another Analysis Another Analysis needs to be done, so go to the “Choose Analyses” Window again

17. Transient Simulation In the “Choosing Analyses” Window, Select tran and Enabled, then set the Stop Time to ‘1.5’, check conservative, and click the Options... button

18. Editing the Transient Simulation In this window, Set the start field to 0, and set the values of the step and maxstep fields to ‘.001’ Then click OK and get back to the simulation Window

19. Simulating Once all of the values are inputted, the only thing left to do is to simulate This can be done by clicking on the Netlist and Run on the side toolbar button This simulation may take a little longer than the previous one

20. The Final Output After the Simulation finishes, a ‘Waveform Window’ will appear with the desired plot on it This plot shows the outputs at the selected pin

21. Understanding the plot Click on the Curves-> Edit and we will be able to set some outputs to off and on to better understand the plot Increase the number of data points by going to “Cadence Analog Environment window” selecting Analysis in the side toolbar and clicking Options and changing the step and max step to get a more accurate plot Changing the voltages may increase or decrease the time the species tested need to approach the detector Change in the mobilities might separate or reduce the space between the two species

22. Finishing up Now we will save the current state of the simulation Go to Session->Save State … and save it as state2

23. Finishing up Now we can close all the open windows