1. Colton Hall, Brendan Lee, David Thomas, Zak Collins, Eli Nelson, Devin Bazata November 1, 2011 Team #7 The Wright Stuff Launch Readiness Review

2. Mission Overview Send up petri dishes containing bacteria Analyze the effects of temperature, pressure, and UV radiation on the bacteria Attempt to capture bacteria in tropopause in a sterile petri dish attached to a servo

3. Design Overview Parallax Standard Servo Arduino Uno Arduino controls servo to deploy bacteria collection dish One petri dish sealed and in heated environment One petri dish sealed and heated but exposed to UV One petri dish unsealed, unheated, and exposed to UV

4. Design Overview

5. Cold Test Test begins Cooler opened to observe servo position Test ends

6. Vacuum Test

7. Bacteria Tests Bacteria confirmed to be "non-pathogenic" Bacteria incubated since last Wednesday Difficulty in having bacteria actually grow during incubation Sterile petri dish was also incubated, and it yielded no bacteria

8. Structural Tests: Whip, Stair, Drop

9. Predicted Results The bacteria in the two petri dishes exposed to the environment will not survive. The bacteria in the petri dish housed inside the satellite will survive. We will be able to retrieve bacteria from the tropopause.

10. Biggest Worries Contamination of the Collection Dish The reliability of the seal on the petri dish controlled by the servo motor. Properly separating variables; maintaining a constant temperature and a constant pressure inside the dish, etc.

11. Requirement Compliance RequirementRequirement Met Mission: test how bacteria survives in near space Four petri dishes containing E. Coli Mission: capture bacteria in tropopause Sterile petri dish attached to servo to collect bacteria Measure temperature and humidity HOBO datalogger flown in BalloonSat Control the servo for deployment in tropopause Arduino Uno program will control the servo Heat interior and two petri dishes to temperature of -10 degrees Celsius Heater system included