2 Standard precaution or Universal Precaution Treat all blood and bodily fluids as if they are contaminated.
3 PPEPersonal Protective Equipment (PPE) should be worn by healthcare personnel to protect themselves against exposure to pathogens, chemical hazards and to minimize potential contamination of their clothing.
4 PPE Use appropriate barrier equipment designed to shield the employee from blood and body fluid contamination.This equipment includes gloves, fluid resistant masksand gowns, splash shields, and eye protection, and isused whenever there is a risk of exposure to blood orother potentially infectious material.
5 Laboratory Apparel or PPE Lab coatGlovesGogglesMaskSplash shieldGownLong hair should be pulled backLoose jewelry should not be worn
6 Common Aseptic Techniques Asepsis – 1.The state of free of living pathogenic microorganisms.2. The process of removing pathogenic microorganisms or protecting against infection by such organisms.TechniquesHandwashing and good personal hygieneUsing disposable gloves when contacting body secretions or contaminated objectsProper cleaning of instruments and equipmentThorough cleaning of the environment
7 Lab Coat…worn by healthcare providers to protect their skin and clothing during patient care and/orhandling specimens. Lab coats worn by phlebotomists and clinical lab personnel are made of fluid-resistant cotton or manmade material. Lab coats are long sleeved, button/snap fronts usually with cotton fitting cuffs. Lab coats cab be disposable or laundrable.
8 Lab Coat Don Lab coat first; button/snap all closures If necessary tie back long hairUpon removing a lab coat; remove gloves first.
9 Practice Lab CoatIt seems silly but practice now putting on your lab coat, buttoning from the neck to the knees.This is the proper way to wear this protective garment.It is often observed with lab coats open and just protecting the top of the shoulders.Remove your lab coat making minimal contact with the possibly contaminated front.
10 Donning and Removal of PPE in the Healthcare Setting The gown is donned firstSecond the face/eye protection equipmentGloves are appliedRemovalGlovesGoggles/face shieldGown
11 GlovesGloves are made from vinyl, latex or nitrile (latex free.) Be sensitive to a possible latex allergy. Gloves are clean and non-sterile.Gloves are the most used PPE in the medical settingThere is no reported difference in the different glove materials and barrier effectiveness when gloves are intact.Intact gloves must be worn and sized to allow the healthcare provider ability to manipulate fine motor skills without the gloves interfering.
12 Gloves should be worn when… performing phlebotomy training.performing all sticks, to include; heel sticks and fingersticks.laboratory testing involves blood or body fluid secretion specimens.handling contaminated items
13 Gloves should be worn when… Gloves should be changed between each patient contact.Touching non-intact skin an/or mucous membranes
14 GlovesAttention should be given when wearing gloves and environmental services that you touch within the laboratory or patient care area.It is important to realize that when you are wearing gloves for protective reasons there is a potential for contamination of other surfaces and other people.
15 GlovesIn the work space be certain to remove gloves (utilizing the proper removal procedure) when answering the telephone, using the restroom, touching door knobs, etc.Another look at gown and glove&feature=results_video&playnext=1&list=PL94 F7D5046DFA210B
16 Donning of GlovesWhen putting on gloves pull gloves up over the cuff or sleeve of the lab coat to provide maximum protection of your skin.
17 Removal of gloves The outside of gloves is contaminated The wrist of one glove is grapped with the opposite hand and peeled off inside out over and off the handHold and ball up removed glove in gloved handSlide the fingers of the non- gloved hand under the wrist of the remaining glove without touching the exterior surface of the glove.
18 Removal of glovesPeel glove off and over first glove ( the first glove should end up inside the second glove with no exterior glove surfaces exposed.)Dispose of gloves in the proper waste container.
19 Removal of Goggles or Face Shield Worn to protect the eyes (googles), nose and mouth from splashes or sprays of body fluidsThe eyes, nose and mouth are the most common pathway of entry for infectious pathogensOutside of goggles of face shield is contaminatedTo remove, handle by head band or ear piecesPlace in designated waste containers to be reprocessed or proper disposable waste container.
20 GownWhen putting on a gown it should fully cover your body from neck to knees. Only inside surfaces should be touched.Slip arms into the protective gown and securely fasten at the neck and waist.Gowning would be utilized by the phlebotomist if a draw was ordered for a patient who is under isolation precautions
21 Removing the Gown The front and sleeves of the gown are contaminated Unfasten the tiesThe gown is removed from the inside out by sliding the arms out of the sleeves.Pull the gown away from the neck and shoulders touching the inside onlyHolding the gown away from your body fold it with the contaminated outside on the insideRoll into a ball and discard in appropriate waste disposal container.
22 Isolation Precautions In some specific Isolation Precaution procedures it may be necessary to wear a respirator. The gown, eye protection and gloves are removed inside the patient area The respirator is not removed until the healthcare provider has left the patient care area.
23 GPSb.Demonstrate and implement proper disposal, storage, and clean up of biohazardous and chemical materials
24 It is the responsibility of the teacher/supervisor to provide training and orientation to the safety elements in the lab and patient care areas.OSHA requires documentation that employees have been properly trained in safety.
25 Exposure Control Methods BiohazardStandard PrecautionsPPEEnvironmental ControlsWork Practice Controls
26 What are the some of the causes of Healthcare Providers contracting an Infection????? Accidental aspirationAccidental inoculation with contaminated needles or syringesSprays from syringesrelease of aerosols when uncapping specimensEquipment accidents ex. centrifugeBroken glassware can yield unintact skinSpilling samples
27 Biological Safety Hood Function as a barrier in the laboratory to prevent the escape of biological aerosols into the lab environment; which is a safety hazard for laboratory personnelA biosafety cabinet (BSC) is not chemical fume hood. Fume hoods are designed to remove chemical fumes and aerosols away from the work area. BSCs are designed to provide both a clean work environment and protection for employees who work with biological hazards. BSCs use vertical laminar airflow to create a barrier to airborne particles, such as microorganisms. They use High Efficiency Particulate Air (HEPA) filters to clean air going into the work area and out to the environment. T
28 Environmental cleaning Ensure that the hospital has adequate procedures for the routine care, cleaning, and disinfection of environmental surfacesDisinfection – wipe work surface area with a 10% chlorine beach solution before and after procedures and when deemed appropriate.
29 Reprocessing of Instruments/ Sterile Services Ensure that reusable equipment is not used for the care of another patient until it has been cleaned and reprocessed appropriately.Examples: scissors, centrifuge carriers should be disinfected
30 Proper Disposal of Sharps Take care to prevent injuries when using needles, scalpels, and other sharp instruments and devices. • Manage health care waste properly to prevent exposures to infections, and toxic effects and injuries to health care personnel, waste handlers, and the community.
31 Spills in the Laboratory PPE: gloves and lab coatAbsorb as much blood or body fluids with disposable towels before decontamination.Clean the spill site with 1:10 chlorine bleach solution of all visible blood/body fluid.Wipe down the spill site with bleach solution soaked disposable towels.Place all disposable materials used to decontaminate and cleanup the spill in an appropriate biohazard waste receptacle.
32 Spills in the Laboratory If nondisposable items were used or affected by the spill soak them overnight in a 10% chlorine bleach solution.Rinse with water and methyl alcohol before use.Glassware and supplies should be autoclaved or incinerated.