1. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Multiply Your Capabilities Leica ChromoPlex TM 1 Dual Detection for Leica BOND

2. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy Leica introduces a new range of detection systems for Leica BOND – ChromoPlex TM

3. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy This new range of multiplex detection systems increases Leica BOND productivity, and demonstrates Leica’s commitment to lead in the provision of quality focused detection systems

4. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy ChromoPlex TM 1 Dual Detection is the first detection system to be launched in this new range

5. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Is a Dual Polymer Detection System with a rapid 1-step staining protocol Double Staining Just Became Easy Leica ChromoPlex 1 Dual Detection for Leica BOND

6. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Detects primary antibody cocktails (Mouse/Rabbit), both self validated and commercially manufactured Leica ChromoPlex 1 Dual Detection for Leica BOND Double Staining Just Became Easy

7. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Allows visualization of different epitopes with two different colored chromogens on the same slide Double Staining Just Became Easy Leica ChromoPlex 1 Dual Detection for Leica BOND

8. Discover The Possibilities Multiply Your Capabilities Maximize Workflow Efficiency Ensure Consistency View Staining Gallery Play All 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection

9. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative Multiply Your Capabilities

10. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with excellent detail Multiply Your Capabilities

11. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative Multiply your capabilities with  Excellent detail of tissue morphology with red and brown chromogens Multiply Your Capabilities

12. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with multiple antibodies Menu Multiply Your Capabilities

13. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Insight for diagnosis with multiple antibodies on the same slide Menu Multiply Your Capabilities

14. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with Clear differentiation Menu Multiply Your Capabilities

15. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Clear color differentiation Menu Multiply Your Capabilities

16. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with antibody cocktails Menu Multiply Your Capabilities

17. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Disease specific antibody cocktails as clinical needs dictate Menu Multiply Your Capabilities

18. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with reduction in turnaround time Menu Multiply Your Capabilities

19. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Perform dual staining within a day’s work with significant reduction in turnaround time Menu Multiply Your Capabilities

20. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Make comparative diagnostics easier Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner. Menu

21. 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Lead the way in diagnostic efficiency Menu Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

22. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Improve the opportunity for same day diagnostics Menu Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

23. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Make Double Staining Routine Practice “the dual staining obtained using the ChromoPlex 1 detection system is attractive, crisp and consistent; ideal when assessing challenging prostatic biopsies.” Dr Geoffrey Hulman MD FRCPath Nottingham University Hospitals NHS Trust

24. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Make Double Staining Routine Practice For the Pathologist who wants to make the most effective use of tissue from core biopsies and small tissue sections, Leica ChromoPlex 1 Dual Detection for Leica BOND will significantly increase the opportunity for same day diagnosis. ChromoPlex is a fully automated IHC system delivering dual chromogen staining in a rapid, one-step process “the dual staining obtained using the ChromoPlex 1 detection system is attractive, crisp and consistent; ideal when assessing challenging prostatic biopsies.” Dr Geoffrey Hulman MD FRCPath, Nottingham University Hospitals NHS Trust

25. Discover The Possibilities Multiply Your Capabilities Maximize Workflow Efficiency Ensure Consistency View Staining Gallery Play All 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection

26. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Maximize workflow Efficiency Now you have the ability to fully automate parallel dual chromogen staining using ChromoPlex 1 Dual Detection for Leica BOND

27. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection 1-step parallel staining Maximize workflow Efficiency with

28. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  1-step parallel staining that reduces turnaround time compared to 2-step sequential staining methods Maximize workflow Efficiency with

29. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection one detection system Maximize workflow Efficiency with

30. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  One detection system, 2 color chromogens Maximize workflow Efficiency with

31. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection validated protocol Maximize workflow Efficiency with

32. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Fully automated, validated protocol Maximize workflow Efficiency with

33. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Leica BOND Covertile™ technology Maximize workflow Efficiency with

34. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Leica BOND Covertile TM technology protects tissue and minimizes damage Maximize workflow Efficiency with

35. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection same day slide delivery Maximize workflow Efficiency with

36. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Improve your opportunity for same day slide delivery for diagnosis Maximize workflow Efficiency with

37. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Simplify staff training Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

38. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Reduce the number of slides requiring staining Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

39. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Increase flexibility to accommodate additional validated staining protocols Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

40. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can Provide rapid service to Pathologists Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

41. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Uninterrupted processing and total automation with Leica BOND and ChromoPlex 1 Dual Detection frees up staff and facilitates effective resource optimization MAXIMIZE WORKFLOW EFFICIENCY > Implement Double Staining on Leica BOND Comparison of automated staining methods Leica BOND Polymer Refine – Single Staining DewaxRetrievalPeroxide BlockAntibodyPost PrimaryPolymerDABHematoxylin Leica ChromoPlex 1 Dual Detection for Leica BOND DewaxRetrievalPeroxide Block Antibody Cocktail Polymer mHPR Polymer rAP DAB Red Chromogen Hematoxylin Single staining Parallel Dual Chromogen staining 165 minutes* 42 minutes* *reference? *Based on 10 slides x 1 SSA. Actual time dependent on sample set-up

42. Discover The Possibilities Multiply Your Capabilities Maximize Workflow Efficiency Ensure Consistency View Staining Gallery Play All 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection

43. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency With the already well established Compact Polymer technology, Leica ChromoPlex 1 Dual Detection for Leica BOND delivers the quality and performance that you associate with Novocastra TM - results that can be relied upon consistently

44. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection intense staining Ensure Consistency with

45. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection  Intense high resolution staining Ensure Consistency with

46. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with enhanced tissue penetration

47. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Enhanced tissue penetration through the use of small multifunctional linkers

48. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with excellent contrast

49. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Excellent contrast between Fast Red and DAB chromogens

50. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with color differentiation

51. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Easy color differentiation

52. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with consistent staining

53. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Uniform reagent application which facilitates clear, consistent differentiated antigen staining

54. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection ENSURE CONSISTENCY > Quality Sensitivity Specificity Leica ChromoPlex 1 Dual Detection for Leica BOND delivers the same quality and performance that you associate with Novocastra products, with  Two chromogens in one detection system  Intense staining with a clean background  Multiple antibodies on a single slide User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

55. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Successfully Implement Double Staining “Historically, we encountered problems with dual staining and efforts to stain with commercial cocktails have been largely unsuccessful. The Leica ChromoPlex 1 detection system, however, enables quick easy staining of both commercial and home-brew cocktails, with excellent results.” Dr Claire Hawkes PhD, CSci Nottingham University Hospitals NHS Trust ENSURE CONSISTENCY >

56. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Successfully Implement Double Staining Leica ChromoPlex 1 Dual Detection for Leica BOND delivers quality, sensitivity and specificity providing you with results that can be relied upon consistently “Historically, we encountered problems with dual staining and efforts to stain with commercial cocktails have been largely unsuccessful. The Leica ChromoPlex 1 detection system, however, enables quick easy staining of both commercial and home-brew cocktails, with excellent results.” Dr Claire Hawkes PhD, CSci - Nottingham University Hospitals NHS Trust ENSURE CONSISTENCY >

57. Discover The Possibilities Multiply Your Capabilities Maximize Workflow Efficiency Ensure Consistency View Staining Gallery Play All 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection

58. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Staining Gallery Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner CLICK A PICTURE TO ENLARGE > Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

59. Gallery 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

60. Gallery 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner

61. Gallery 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner

62. Gallery 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner

63. Menu 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection Staining Gallery Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner CLICK A PICTURE TO ENLARGE > Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

64. Discover The Possibilities Multiply Your Capabilities Maximize Workflow Efficiency Ensure Consistency View Staining Gallery Play All 95.10782 Rev B 09/2012 ChromoPlex 1 Dual Detection

65. Thank you Leica ChromoPlex TM 1 Dual Detection for Leica BOND Multiply Your Capabilities