1. Biosynthetic trafficking of TbCatL is TbERP dependent.
Biosynthetic trafficking of TbCatL is TbERP dependent. Specific dsRNA was induced for 72 h in each of the BSF TbERP RNAi cell lines as indicated. Control (Tet-, open symbols) and induced (Tet+, closed symbols) cells were pulse (10 min)-chase (2 h) radiolabeled, and TbCatL trafficking (conversion to the mature form) was analyzed over time as described in Materials and Methods. TbCatL was specifically immunoprecipitated from total cell lysates at the indicated chase times and analyzed by SDS-PAGE and phosphorimaging (1 × 107 cell equivalents per lane). (Top) Quantification of TbCatL isoforms normalized to total initial TbCatL (mean ± SEM for n = 3). Squares, mature TbCatL (M); circles, immature TbCatL forms (I+X). (Bottom) Representative phosphorimages are presented. Tet+ cultures were sampled at later time points to allow for more complete conversion of TbCatL.
Emilia K. Kruzel et al. mSphere 2017; doi: /mSphere